cole1 origin Search Results


cole1 origin  (Addgene inc)
91
Addgene inc cole1 origin
Cole1 Origin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cole1 origin/product/Addgene inc
Average 91 stars, based on 1 article reviews
cole1 origin - by Bioz Stars, 2026-04
91/100 stars
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cole1 origins  (Twist Bioscience)
90
Twist Bioscience cole1 origins
a , Experimental workflow for validating generated origins. b , Assessment of <t>ColE1-type</t> origin function in E. coli . Matched random sequences contain the same number of mutations relative to wild-type as their corresponding OriGen-generated sequences. Lighter green indicates a single nucleotide polymorphism or single nucleotide indel was detected after sequencing. Stars represent unsuccessful experiments, which are in the process of being rerun . c , RNAI secondary structure comparison of one OriGen-generated ColE1 example vs closest WT vs matched random mutant control. d , Assessment of uncharacterized Rep3-type origin function in E. coli . e , Distribution of known motifs in examples of Rep3 OriGen-generated origins vs closest WT vs matched random mutant. In both examples, the model-generated origins led to replication while the random mutants did not.
Cole1 Origins, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cole1 origins/product/Twist Bioscience
Average 90 stars, based on 1 article reviews
cole1 origins - by Bioz Stars, 2026-04
90/100 stars
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cole1 origin  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd cole1 origin
Strains and plasmids used in this study
Cole1 Origin, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cole1 origin/product/Amersham Pharmacia Biotech Ltd
Average 90 stars, based on 1 article reviews
cole1 origin - by Bioz Stars, 2026-04
90/100 stars
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cole1-type origin  (Verlag GmbH)
90
Verlag GmbH cole1-type origin
Strains and plasmids used in this study
Cole1 Type Origin, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cole1-type origin/product/Verlag GmbH
Average 90 stars, based on 1 article reviews
cole1-type origin - by Bioz Stars, 2026-04
90/100 stars
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atp-dependent resolution of r-loops at the cole1 replication origin by escherichia coli recg protein  (Shinagawa Corporation)
90
Shinagawa Corporation atp-dependent resolution of r-loops at the cole1 replication origin by escherichia coli recg protein
Strains and plasmids used in this study
Atp Dependent Resolution Of R Loops At The Cole1 Replication Origin By Escherichia Coli Recg Protein, supplied by Shinagawa Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atp-dependent resolution of r-loops at the cole1 replication origin by escherichia coli recg protein/product/Shinagawa Corporation
Average 90 stars, based on 1 article reviews
atp-dependent resolution of r-loops at the cole1 replication origin by escherichia coli recg protein - by Bioz Stars, 2026-04
90/100 stars
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ptu2s a cole1 origin plasmid 74091  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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ptu2s b cole1 origin plasmid 74092  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
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Image Search Results


a , Experimental workflow for validating generated origins. b , Assessment of ColE1-type origin function in E. coli . Matched random sequences contain the same number of mutations relative to wild-type as their corresponding OriGen-generated sequences. Lighter green indicates a single nucleotide polymorphism or single nucleotide indel was detected after sequencing. Stars represent unsuccessful experiments, which are in the process of being rerun . c , RNAI secondary structure comparison of one OriGen-generated ColE1 example vs closest WT vs matched random mutant control. d , Assessment of uncharacterized Rep3-type origin function in E. coli . e , Distribution of known motifs in examples of Rep3 OriGen-generated origins vs closest WT vs matched random mutant. In both examples, the model-generated origins led to replication while the random mutants did not.

Journal: bioRxiv

Article Title: Generating functional plasmid origins with OriGen

doi: 10.1101/2025.02.03.636306

Figure Lengend Snippet: a , Experimental workflow for validating generated origins. b , Assessment of ColE1-type origin function in E. coli . Matched random sequences contain the same number of mutations relative to wild-type as their corresponding OriGen-generated sequences. Lighter green indicates a single nucleotide polymorphism or single nucleotide indel was detected after sequencing. Stars represent unsuccessful experiments, which are in the process of being rerun . c , RNAI secondary structure comparison of one OriGen-generated ColE1 example vs closest WT vs matched random mutant control. d , Assessment of uncharacterized Rep3-type origin function in E. coli . e , Distribution of known motifs in examples of Rep3 OriGen-generated origins vs closest WT vs matched random mutant. In both examples, the model-generated origins led to replication while the random mutants did not.

Article Snippet: ColE1 origins were synthesized as gene fragments (Twist Biosciences) containing BsaI sites and complementary overhangs (Supplemental Table 3).

Techniques: Generated, Sequencing, Comparison, Mutagenesis, Control

Strains and plasmids used in this study

Journal:

Article Title: Effect of Sequences of the Active-Site Dipeptides of DsbA and DsbC on In Vivo Folding of Multidisulfide Proteins in Escherichia coli

doi: 10.1128/JB.183.3.980-988.2001

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: pTrc99A , trc promoter, Amp r , ColE1 origin , Amersham Pharmacia Biotech.

Techniques: Plasmid Preparation, Mutagenesis, Expressing, Clone Assay