Journal: Inflammatory bowel diseases

Article Title: miR-4728-3p functions as a tumor suppressor in ulcerative colitis-associated colorectal neoplasia through regulation of focal adhesion signaling

doi: 10.1097/MIB.0000000000001104

Figure Lengend Snippet: Expression correlation between miR-4728-3p and CAV1 (A), COL1A2 (B), and THBS2 (C) by microarray analysis. Quantitative real-time PCR was performed to validate expression of miR-4728-3p (D) as well as CAV1, COL1A2, and THBS2 (E) in the same 42 patients analyzed by microarray. Quantitative real-time PCR was also performed in 11 UC-associated cancers, nondysplastic adjacent tissue, and normal-appearing control tissues for miR-4728-3p (F) as well as CAV1, COL1A2, and THBS2 (G). Error bars represent standard error of the mean.

Article Snippet: Primary antibodies were diluted to 1:100 for CAV1 (Santa Cruz Biotechnology), 1:500 for COL1A2 (Novus Biologicals), 1:200 for THBS2 (Santa Cruz Biotechnology), and 1:5000 for beta actin (Sigma Aldrich).

Techniques: Expressing, Microarray, Real-time Polymerase Chain Reaction, Control

Journal: Inflammatory bowel diseases

Article Title: miR-4728-3p functions as a tumor suppressor in ulcerative colitis-associated colorectal neoplasia through regulation of focal adhesion signaling

doi: 10.1097/MIB.0000000000001104

Figure Lengend Snippet: A) Representative Western blots and B) quantitative expression by densitometry of CAV1, COL1A2, and THBS2 in the indicated colon cancer cell line following transfection with miR-4728-3p or scrambled oligo (n=3/treatment group). C) Firefly luciferase activity after transfection with miR-4728-3p and following treatment of indicated genes regulated by wild type or mutant 3′UTR (n=6/treatment group). To control for transfection efficiency, firefly luciferase activity was normalized to renilla luciferase activity. D) Representative images of wound healing in HCT116 cells treated with miR-4728-3p or a scrambled probe. E) Quantitative measurement of cell migration to assess wound closure in HCT116 cells treated with miR-4728-3p or a scrambled probe (values are means ± SD of 3 independent platings). F) Representative immunofluorescence images of vinculin and actin to assess focal adhesion complexes and actin cytoskeleton in HCT116 cells following treatment with miR-4728-3p or a scrambled probe. G) Focal adhesion intensity and H) area following treatment with miR-4728-3p or a scrambled probe (n=3 wells with 12 measurements each/treatment group). Error bars represent standard deviation.

Article Snippet: Primary antibodies were diluted to 1:100 for CAV1 (Santa Cruz Biotechnology), 1:500 for COL1A2 (Novus Biologicals), 1:200 for THBS2 (Santa Cruz Biotechnology), and 1:5000 for beta actin (Sigma Aldrich).

Techniques: Western Blot, Expressing, Transfection, Luciferase, Activity Assay, Mutagenesis, Control, Migration, Immunofluorescence, Standard Deviation

Journal: Journal of the mechanical behavior of biomedical materials

Article Title: Shape-fitting collagen-PLA composite promotes osteogenic differentiation of porcine adipose stem cells

doi: 10.1016/j.jmbbm.2019.03.017

Figure Lengend Snippet: Primers used for TAQMAN gene expression analyses.

Article Snippet: COL1A2 , Ss03375009_u1.

Techniques: Gene Expression

Journal: Journal of the mechanical behavior of biomedical materials

Article Title: Shape-fitting collagen-PLA composite promotes osteogenic differentiation of porcine adipose stem cells

doi: 10.1016/j.jmbbm.2019.03.017

Figure Lengend Snippet: Gene expression profiles of RUNX2, BMP2, BGLAP, Osterix, COL1A2, and MMP9 for pASCs within the mineralized collagen scaffold vs. collagen-PLA composite. * indicates significant (p < 0.05) higher gene expression between the two groups on the same day. ^ indicates significantly (p < 0.05) higher gene expression of one group compared to the same group on day 1. # indicates significantly (p < 0.05) lower gene expression of one group compared to the same group on day 1. Data expressed as mean ± standard deviation (n=5).

Article Snippet: COL1A2 , Ss03375009_u1.

Techniques: Gene Expression, Standard Deviation

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Reconstruction of human elastic cartilage by a CD44 + CD90 + stem cell in the ear perichondrium

doi: 10.1073/pnas.1109767108

Figure Lengend Snippet: Tissue reconstitution capability of human perichondrocytes. (A and I) Chondrocytes (Left) and perichondrocytes (Right) formed cartilage-like tissues 3 mo after s.c. transplantation. (Scale bars, 1 mm.) (B and J) Hematoxylin/eosin, (C, D, K, and L) Alcian blue, (E and M) Toluidine blue, and (F and N) Safranin O staining showed that the reconstructed tissue contained mature chondrocytes in lacunae that produced high levels of proteoglycan. (G and O) Elastica Van Gieson staining showed that the reconstructed cartilage contained elastic fibers. (H and P) Immunohistochemistry revealed that perichondrocyte-derived cartilage (Right) contained type I collagen (Col1)+ capsules that enveloped a type II collagen (Col2)+ chondrium layer. (Scale bars, 200 μm.)

Article Snippet: The following quantitative PCR (qPCR) primers were used in TaqMan gene expression assays: COL1A1 , Hs00266273_ml; COL2A1 , Hs00164099_ml; CSPG2 , Hs01007933_m1; ELN , Hs00355783_ml; and FBN1 , Hs00171191_m1 (Applied Biosystems).

Techniques: Transplantation Assay, Staining, Produced, Immunohistochemistry, Derivative Assay, Capsules