Journal: Turkish Journal of Emergency Medicine

Article Title: Effect of smartphone applications on cardiopulmonary resuscitation quality metrics in a mannequin study: A randomized trial

doi: 10.4103/2452-2473.313333

Figure Lengend Snippet:

Article Snippet: A mobile phone (Apple iPhone 4S) and a downloaded CPR feedback App (Pocket-CPR-Zoll Medical Corporation, Chelmsford, MA) were employed in this study.

Techniques:

Journal: Globalization and Health

Article Title: International comparison of pharmaceutical industry payment disclosures in the UK and Japan: implications for self-regulation, public regulation, and transparency

doi: 10.1186/s12992-022-00902-9

Figure Lengend Snippet: Aggregated payments – company-level data reported by companies following the ABPI Code (2018)

Article Snippet: Finally, an ex-employee revealed that Indivior, a company that has ratified the ABPI Code, did not disclose any 2017 payments, allegedly because it did not know it had agreed to disclose them since becoming an official “non-member” in 2017 [ ].

Techniques:

Journal: Biochemical and biophysical research communications

Article Title: Microscopic analysis of Orai-mediated store-operated calcium entry in cells with experimentally altered levels of amyloid precursor protein.

doi: 10.1016/j.bbrc.2016.08.072

Figure Lengend Snippet: Fig. 1. Generation of Jurkat cell lines stably producing APP. (A) Immunoblot of endogenous APP in Jurkat, 293T/17, and HeLa cells carrying APP-targeting shRNA (APP KD) or control shRNA. (B) qPCR analysis of relative APP mRNA levels in cells presented in (A). Differences from the reference level of 1 set for HeLa control cells were analyzed using one-sample t-test (n ¼ 4 each). Significant differences are marked with asterisks (***p < 0.001). (C) Immunoblot of APP, Orai1, and STIM1 in Jurkat cells stably expressing APP, APPSWE or an empty cassette (control), compared with wildtype HeLa cells. (D) FACS analysis of Y188-labeled Jurkat APP cells and APPSWE cells. Y188-labeled and mock-labeled 293T/17 cells were analyzed in parallel to set a threshold for Y188- positive cells (horizontal line). The x-axis of both histograms shows the Y188 signal normalized to cell size (FL1-H/FSC) on a logarithmic scale.

Article Snippet: Human APP and ORAI1 coding sequences originated from Addgene plasmids #30137, #30145 and #22754, and were cloned MluI-NotI into a modified pLVTH vector (Addgene #12262), in which eGFP and the H1 promoter were replacedwith an IRES-PURO-WPRE cassette.

Techniques: Stable Transfection, Western Blot, shRNA, Control, Expressing, Labeling

Journal: Biochemical and biophysical research communications

Article Title: Microscopic analysis of Orai-mediated store-operated calcium entry in cells with experimentally altered levels of amyloid precursor protein.

doi: 10.1016/j.bbrc.2016.08.072

Figure Lengend Snippet: Fig. 2. Microscopic analysis of SOCE in APP-producing Jurkat cells. (A) SOCE measurements in Fura-4F-loaded Jurkat cells that produced APP (n ¼ 13), APPSWE (n ¼ 12) and control cells with an empty cassette (n ¼ 13). (B) SOCE measurements in Jurkat cells that produced dominant-negative Orai1 (Orai1 DN; n ¼ 5) and control cells (n ¼ 5). Shown are averaged traces from representative experiments and calculated mean values with standard error. Differences from control cells were analyzed using unpaired t-test, and significant dif- ferences are marked with asterisks (***p < 0.001).

Article Snippet: Human APP and ORAI1 coding sequences originated from Addgene plasmids #30137, #30145 and #22754, and were cloned MluI-NotI into a modified pLVTH vector (Addgene #12262), in which eGFP and the H1 promoter were replacedwith an IRES-PURO-WPRE cassette.

Techniques: Produced, Control, Dominant Negative Mutation

Journal: Biochemical and biophysical research communications

Article Title: Microscopic analysis of Orai-mediated store-operated calcium entry in cells with experimentally altered levels of amyloid precursor protein.

doi: 10.1016/j.bbrc.2016.08.072

Figure Lengend Snippet: Fig. 3. Analysis of SOCE in Jurkat cells producing C99 fragments. (A) Immunoblot of small APP fragments (detected with Y188 antibody) in Jurkat cells stably expressing APPSWE, C99 cDNA, or an empty cassette (control) compared with wildtype HeLa cells. DAPT treatment (5 mM for 5 h) is indicated below the gels. (B) Immunoblot of APP, Orai1, and STIM1 in Jurkat cells stably expressing C99 cDNA, C99IND cDNA, or an empty cassette (control) compared with wildtype HeLa cells. (C) qPCR analysis of ORAI1 and STIM1 mRNA levels in C99- producing Jurkat cells relative to those in Jurkat control cells. Differences were analyzed using one-sample t-test (n ¼ 3 each). (D) SOCE measurements in Fura-4F-loaded Jurkat C99 cells (n ¼ 11), C99IND cells (n ¼ 11) and control cells (n ¼ 12). Traces are from representative experiments. The data were analyzed using unpaired t-test.

Article Snippet: Human APP and ORAI1 coding sequences originated from Addgene plasmids #30137, #30145 and #22754, and were cloned MluI-NotI into a modified pLVTH vector (Addgene #12262), in which eGFP and the H1 promoter were replacedwith an IRES-PURO-WPRE cassette.

Techniques: Western Blot, Stable Transfection, Expressing, Control

Journal: Biochemical and biophysical research communications

Article Title: Microscopic analysis of Orai-mediated store-operated calcium entry in cells with experimentally altered levels of amyloid precursor protein.

doi: 10.1016/j.bbrc.2016.08.072

Figure Lengend Snippet: Fig. 4. Analysis of SOCE in HeLa cells depleted of APP. (A) SOCE measurements in Fura-2-loaded HeLa cells stably expressing APP-targeting shRNA (APP KD; n ¼ 8), ORAI1-targeting shRNA (ORAI1 KD; n ¼ 3) and control shRNA (control; n ¼ 8). The stores were depleted with 20 mM CPA in Ca2þ-free buffer containing 0.5 mM EGTA for 400 s, and SOCE was initiated by the re-addition of 2 mM Ca2þ. Shown are averaged (black) and individual (gray) SOCE traces from representative experiments. The data were analyzed using unpaired t- test, and significant differences from controls are marked with asterisks (***p < 0.001). (B) qPCR analysis of ORAI1 and STIM1 mRNAs in cells presented in (A). Differences from the reference level of 1 set for controls were analyzed using one-sample t-test (n ¼ 3 each). Statistical significance is marked with asterisks (***p < 0.001).

Article Snippet: Human APP and ORAI1 coding sequences originated from Addgene plasmids #30137, #30145 and #22754, and were cloned MluI-NotI into a modified pLVTH vector (Addgene #12262), in which eGFP and the H1 promoter were replacedwith an IRES-PURO-WPRE cassette.

Techniques: Stable Transfection, Expressing, shRNA, Control

Journal: Human-animal interactions

Article Title: Feasibility of using ecological momentary assessment to measure the effects of interactions with pet dogs on psychophysiological reactivity in adolescents with social anxiety

doi: 10.1079/hai.2023.0036

Figure Lengend Snippet: Example of a full day of EDA/SCL data from two participants integrated with EMA activity markers: (a) example of participant EDA data with EMA interaction markers; and (b) example of participant EDA data with E4 and EMA interaction markers.

Article Snippet: Consenting participants were mailed a data collection package, which included printed study protocol instructions (including a QR code enabling download of the EMA mobile phone app), an Empatica E4 sensor (which is a wearable wristband) with Biopac electrodes (see for additional information), and a stamped and addressed return envelope for the study materials.

Techniques: Activity Assay