Journal: International journal of molecular sciences

Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import.

doi: 10.3390/ijms251910832

Figure Lengend Snippet: Figure 1. (A) Protein levels of MPV17 in cardiomyocytes treated with FAC evaluated by WB; (B) the overexpression of MPV17 in cardiomyocytes was detected by WB; (C) cell death labeled with PI staining in each group; (D) the knockdown of MPV17 in cardiomyocytes by siRNA was detected by WB; (E) cell death labeled with PI staining in each group; (F) C11-BO was applied to label the lipid peroxides and flow cytometry was used for the detection of green fluoresce in each group; (G) C11-BO was applied to label the lipid peroxides and flow cytometry was used for the detection of green fluoresce in each group; (H) ACSL4, FTH–1 and GPX–4 were evaluated by WB; (I) ACSL4, FTH–1 and GPX–4 were evaluated by WB; *, p < 0.05 was statistically significant.

Article Snippet: The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).

Techniques: Over Expression, Labeling, Staining, Knockdown, Flow Cytometry

Journal: International journal of molecular sciences

Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import.

doi: 10.3390/ijms251910832

Figure Lengend Snippet: Figure 2. (A) Prussion Blue staining for the examination of iron levels in cardiac tissues; scale bar, 60 µm; (B) cell death labeled with PI staining in cardiac tissues after I/R surgery; red, PI; blue, DAPI; green, Actinin; scale bar, 60 µm; (C) statistic data of the relative MDA levels; (D) the expression of ACSL4, FTH–1 and GPX–4 were checked by WB; (E) cell death labeled with PI staining in cardiac tissues after I/R surgery; red, PI; blue, DAPI; green, Actinin; scale bar, 60 µm; (F) Masson trichrome staining for the examination of collagen in the heart tissue; (G) LVIDd, diastolic left ventricular internal diameter; (H) EF, the ejection fraction of the left ventricular diameter; FS, the fractional shortening of the left ventricular diameter; (I) statistic data of the relative MDA levels in each group; (J) the expression of MPV17, ACSL4, FTH–1 and GPX–4 were checked by WB; *, p < 0.05 was statistically significant; ns, none significance.

Article Snippet: The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).

Techniques: Staining, Labeling, Expressing

Journal: International journal of molecular sciences

Article Title: Role of Acyl-CoA Thioesterase 7 in Regulating Fatty Acid Metabolism and Its Contribution to the Onset and Progression of Bovine Clinical Mastitis.

doi: 10.3390/ijms252313046

Figure Lengend Snippet: Figure 4. Distribution analysis of ACOT7 in mammary glands of the two groups. (A) Pathological variation in bovine mammary glands of the healthy control group (Con/C; A1) and clinical mastitis groups (CM; A2) based on H&E staining. (B) ACOT7 distribution in bovine mammary glands of the Con/C (B1) and CM (B2) groups based on immunohistochemical staining. (C) Negative control (NC) for Con/C (C1) and CM (C2) groups. (D) Gray values of positive expression of ACOT7 protein quantified using ImageJ 1.44p software. Con/C, control group. CM, clinical mastitis group. NC, negative control; MA: mammary alveoli; MECs, mammary epithelial cells; NEUT, neutrophil. Scale bars, 50 µm (200× magnification). ** represents p < 0.01.

Article Snippet: The slices were incubated with a rabbit anti-ACOT7 primary antibody at a 1:200 dilution (Proteintech, Wuhan, China).

Techniques: Control, Staining, Immunohistochemical staining, Negative Control, Expressing, Software

Journal: International journal of molecular sciences

Article Title: Role of Acyl-CoA Thioesterase 7 in Regulating Fatty Acid Metabolism and Its Contribution to the Onset and Progression of Bovine Clinical Mastitis.

doi: 10.3390/ijms252313046

Figure Lengend Snippet: Figure 5. Co-localization analysis of ACOT7 in mammary glands of the two groups. (A–D) Co- localization analysis of ACOT7 in bovine mammary glands of the Con/C and CM groups: nuclei (blue, A1,A2), CK-18 (red, B1,B2), ACOT7 (green, C1,C2), merged with CK18 and ACOT7 (D1,D2) staining, respectively. (E) Positive IF signals of ACOT7 protein quantified using ImageJ software. Con/C, control group. CM, clinical mastitis group. MA, mammary alveoli; MECs, mammary epithelial cells; Scale bars, 50 µm (200× magnification). ** represents p < 0.01.

Article Snippet: The slices were incubated with a rabbit anti-ACOT7 primary antibody at a 1:200 dilution (Proteintech, Wuhan, China).

Techniques: Staining, Software, Control

Journal: International journal of molecular sciences

Article Title: Role of Acyl-CoA Thioesterase 7 in Regulating Fatty Acid Metabolism and Its Contribution to the Onset and Progression of Bovine Clinical Mastitis.

doi: 10.3390/ijms252313046

Figure Lengend Snippet: Figure 6. Expression patterns of ACOT7 mRNA and protein in mammary glands of the two groups. (A) Relative expression level of ACOT7 mRNA, monitored via qRT-PCR assays. (B) Protein bands and relative expression level of ACOT7, monitored via Western blot assays and using ImageJ software, respectively. Con/C, control group. CM, clinical mastitis group. Data are presented as means ± SEM. ** represents p < 0.01.

Article Snippet: The slices were incubated with a rabbit anti-ACOT7 primary antibody at a 1:200 dilution (Proteintech, Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Software, Control

Journal: International journal of molecular sciences

Article Title: Role of Acyl-CoA Thioesterase 7 in Regulating Fatty Acid Metabolism and Its Contribution to the Onset and Progression of Bovine Clinical Mastitis.

doi: 10.3390/ijms252313046

Figure Lengend Snippet: Figure 7. Proposed molecular mechanism underlying the effects of ACOT7 in the mammary glands of dairy cows with clinical mastitis (CM). MECs, mammary epithelial cells; FABP, fatty-acid-binding protein; FATP, fatty acid transport protein; TLR4, Toll-like receptor 4; TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; LPS, lipopolysaccharide. Scale bar, 50 µm (200× magnification).

Article Snippet: The slices were incubated with a rabbit anti-ACOT7 primary antibody at a 1:200 dilution (Proteintech, Wuhan, China).

Techniques: Binding Assay

Journal: Environment international

Article Title: Cresyl diphenyl phosphate (a novel organophosphate ester) induces hepatic steatosis by directly binding to liver X receptor α: From molecule action to risk assessment.

doi: 10.1016/j.envint.2024.109168

Figure Lengend Snippet: Fig. 6. Lipogenesis effects of CDP on mouse liver. a-h 6-week-old mice were intragastric administrated with different dose of CDP (0.1, 1, 10 mg/kg/day) for 12 weeks with corn oil as control treatment (n = 7 per group). (a) H&E and Oil red O staining of liver sections. (b-f) Relative Lxrα, Srebp1c, Acc1, Fasn, and Scd mRNA expression levels in mouse liver. The relative mRNA level was normalized to control group (mice treated with corn oil). *P < 0.05, compared with control. (g) Protein expression of LXRα, SREBP1c, ACC1, FASN, and SCD in mouse liver. (h) Quantitative analysis of protein expression.

Article Snippet: The following antibodies were used in the study: anti-LXRα antibody (Proteintech, catalogue no. 14351-1-AP, diluted 1:5000), anti-SREBP1c antibody (Zen bio, catalogue no. 347061, diluted 1:1000), anti-FASN antibody (Zen bio, catalogue no. 200194, diluted 1:1000), anti-ACC1 antibody (Proteintech, catalogue no. 21923-1-AP, diluted 1:5000), anti-SCD antibody (Zen bio, catalogue no. R25675, diluted 1:1000).

Techniques: Control, Staining, Expressing

Journal: The journals of gerontology. Series A, Biological sciences and medical sciences

Article Title: Resveratrol improves insulin resistance hyperglycemia and hepatosteatosis but not hypertriglyceridemia, inflammation, and life span in a mouse model for Werner syndrome.

doi: 10.1093/gerona/glq184

Figure Lengend Snippet: Figure 7. Resveratrol effect on energy production (ATP) and AMPKa activity of 9-month-old WrnDhel/Dhel mice in liver tissues. (A) Liver GSH levels in 9-month- old wild-type (WT), WrnDhel/Dhel, and resveratrol-treated WrnDhel/Dhel mice. Measurements were taken from four mice of each group. (B) Liver ATP levels in 9-month- old WT, WrnDhel/Dhel, and resveratrol-treated WrnDhel/Dhel mice. (C) Example of Western blots showing levels of AMPKa phosphorylation at its threonine 172 (p-AMPKa) in liver tissues of all three cohorts. The graph represents scanning analyses of Western blots expressed as the average signal from the phosphorylated AMPKa over total AMPKa detected in the liver of mice. (D) Example of Western blots showing levels of acetyl-CoA carboxylase phosphorylation at its serine 79 (p-ACC) in liver tissues of all three cohorts. The graph represents scanning analyses of Western blots expressed as the average signal from the phosphorylated ACC over total ACC detected in the liver of mice. (E) Example of Western blots showing levels of hormone-sensitive lipase (HSL) phosphorylation at its serine 565 (p-HSL) in liver tissues of all three c ohorts. The graph represents scanning analyses of Western blots expressed as the average signal from the phosphorylated HSL over total HSL detected in the liver of mice. Measurements were taken from four to six males in each group. Bars in the histograms represent the SEM. (* = unpaired t test; p < .05 when compared with WrnDhel/Dhel mice). (F) Example of Western blots showing levels of fatty acid synthase (FASN) in liver tissues of all three cohorts. The gene product HNRPK was used as loading control. The graph on the right represents scanning analyses of Western blots expressed as the average signal from the FASN signal over HNRPK signal detected in the liver of mice. Measurements were taken from three males in each group. Bars in the histograms represent the SEM. (* = unpaired t test; p < .02 when compared with WrnDhel/Dhel mice).

Article Snippet: The rabbit monoclonal antibodies against AMPKa (23A3), against AMPKa phosphorylated on threonine 172 (40H9), against acetyl-CoA carboxylase (C83B10), against phosphorylated acetyl-CoA carboxylase on Serine 79, against the hormone-sensitive lipase (HSL), and against the phosphorylated HSL on Serine 565 were purchased from Cell Signaling Technology (Danvers, MA).

Techniques: Activity Assay, Western Blot, Phospho-proteomics, Control