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Image Search Results
Journal: PLoS ONE
Article Title: Engrafted Human Induced Pluripotent Stem Cell-Derived Anterior Specified Neural Progenitors Protect the Rat Crushed Optic Nerve
doi: 10.1371/journal.pone.0071855
Figure Lengend Snippet: Retrograde labeling by intracollicular injection of DiI showed labeled retinal ganglion cells (RGCs) in the retina. (A) Representative micrographs of retrograde-labeled RGCs in different experimental groups including (A I ) animals with intact optic nerve; (A II ) animals with optic nerve crush (ONC); (A III ) animals with ONC and transplanted cells; and (A IV ) animals with ONC and treated with CM. For higher magnification images, please see . (B) Quantitative analysis of RGC survival as number of cells/mm 2 in the retina of different groups on day 21 post-ONC, n = 4, a: p< 0.05 as determined by student t -test. Compared to the intact nerve, a few cells were labeled in the eye with vehicle-transplanted nerve following the crush. Both hiPSC-NPs transplantation and CM of cells (CM-hiPSC-NPs) protected RGCs and increased the remaining cell population. (C) CM of hiPSC-NPs evaluated by ELISA for the secretion of trophic factors. CNTF, FGF2 and IGF1 were released into the medium. Data are expressed as mean±SEM, n = 9 independent experiments.
Article Snippet: To evaluate growth factor secretion by hiPSC-NPs, we quantified the CM of NPs for protein levels of ciliary neurotrophic factor (CNTF), bFGF and insulin-like growth factor 1 (IGF1) using specific
Techniques: Labeling, Injection, Transplantation Assay, Enzyme-linked Immunosorbent Assay