Journal: Addiction biology

Article Title: Optogenetic brain-stimulation reward: A new procedure to re-evaluate the rewarding versus aversive effects of cannabinoids in dopamine transporter-Cre mice.

doi: 10.1111/adb.13005

Figure Lengend Snippet: FIGURE 6 CB2R-immunostaining in different phenotypes of neurons in the ventral tegmental area (VTA), illustrating CB2R-immunostaining in VTA TH-positive DA neurons A, but not in VTA vGluT2-positive glutamate neurons B or GAD67-positive GABA neurons C. BL, baseline responding in the absence of drug treatment

Article Snippet: Dual-labeling IHC was performed using a CB2R antibody (Alomone, #ACR-002, 1:250) and an anti-tyrosine hydroxylase (anti-TH) monoclonal antibody (1:500; Millipore, Billerica, MA, USA).

Techniques: Immunostaining

Journal: Addiction biology

Article Title: Optogenetic brain-stimulation reward: A new procedure to re-evaluate the rewarding versus aversive effects of cannabinoids in dopamine transporter-Cre mice.

doi: 10.1111/adb.13005

Figure Lengend Snippet: FIGURE 7 Diagram summarizing CB1R and CB2R expression in VTA dopaminergic (DA), glutamatergic, and GABAergic neurons. Cannabinoids may bind to CB1R in GABAergic neurons, producing rewarding or reward-enhancing effects. Conversely, cannabinoids may also bind to CB1R on VTA glutamate neurons or glutamatergic afferents, or to CB2R on DA neurons, producing aversive or reward-attenuating effects. The final subjective effect depends on the balance of both opposite actions. NAc, nucleus accumbens; oICSS, optogenetic intracranial self- stimulation; VTA, ventral tegmental area

Article Snippet: Dual-labeling IHC was performed using a CB2R antibody (Alomone, #ACR-002, 1:250) and an anti-tyrosine hydroxylase (anti-TH) monoclonal antibody (1:500; Millipore, Billerica, MA, USA).

Techniques: Expressing

Journal: Journal of Biological Chemistry

Article Title: The Endocannabinoid/Endovanilloid N-Arachidonoyl Dopamine (NADA) and Synthetic Cannabinoid WIN55,212-2 Abate the Inflammatory Activation of Human Endothelial Cells

doi: 10.1074/jbc.m113.536953

Figure Lengend Snippet: FIGURE 2. ECs express CBR and TRPV1 proteins. HUVEC, HMVEC-brain, -liver, and -lung, and HCAEC were grown to confluency in 6-well plates and lysed with RIPA-LB. CB1R (A), CB2R (B), GPR18 (C), GPR55 (D), and TRPV1 (E) protein levels were assessed by immunoblot using the indicated antibodies. PBMCs were immediately lysed in RIPA-LB upon isolation. -Actin levels were assessed as a loading control (bottom panels) (A–E). Arrows indicate candidate CBR or TRPV1 protein bands. For reference, the predicted molecular weight and the molecular weight indicated in the respective products sheets are shown.

Article Snippet: The antibodies used were as follows: CB1R (2 g/ml, AP01265PU-N, Acris Antibodies, San Diego); CB2R (1 g/ml, ARP63486_P050, Aviva Systems Biology, San Diego); GPR18 (2 g/ml, ab76258, Abcam, Cambridge, MA); GPR55 (0.8 g/ml, orb101191, Biorbyt, Cambridge, UK); TRPV1 (1 g/ml, ab111973, Abcam); and -actin (0.1 g/ml, A2066, Sigma).

Techniques: Western Blot, Isolation, Control, Molecular Weight

Journal: Journal of Pain Research

Article Title: Expression of matrix metalloproteinases and components of the endocannabinoid system in the knee joint are associated with biphasic pain progression in a rat model of osteoarthritis

doi: 10.2147/JPR.S132682

Figure Lengend Snippet: Correlation matrix for DWB data and expression levels

Article Snippet: Cannabinoid receptor 2 , Cb2 , Rn04342831_s1 , 847 (NM_020543.4) , 99.

Techniques: Expressing, Gene Expression

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: CB 2 activation reduces the number of infected cells without altering HIV receptor expression. a , b. Bulk RNA-seq analysis showing the expression of key endocannabinoid system genes in ( a ) MDMs and ( b ) iMg. c. Representative images of iMg immunostained for CD4 (red), CCR5 (green), and DAPI (blue). d. Quantification of CD4 and CCR5 expression. Each symbol represents the average of 2 wells/condition. n = 3 differentiations from 2 iMg lines. e. Representative images of iMg immunostained for HIV p24. f. Quantification of CD4 and CCR5 expression. Each symbol represents the average of 2 wells/condition. n = 3 differentiations from 2 iMg lines. One-way analysis of variance (ANOVA) with Dunnett’s correction for multiple comparisons. * p < 0.05; ns, not significant

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: Activation Assay, Infection, Expressing, RNA Sequencing

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: CB 2 agonists dose-dependently reduce HIV replication in macrophages and microglia. a , b. HIV p24 levels in supernatants of monocyte-derived macrophages (MDMs, n = 7) ( a ) and human induced pluripotent stem cell-derived microglia (iMg, n = 3) ( b ), normalized to the HIV + vehicle. Two-way ANOVA with Dunnett’s correction for multiple comparisons. * p < 0.05, ** p < 0.01. c , d. Bulk RNA-seq data showing count/million reads of key endocannabinoid system genes in MDMs ( c ) and iMg ( d )

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: Derivative Assay, RNA Sequencing

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: CB 2 agonists reduce global cytokine release following HIV infection in MDMs but not in iMg. a , b. Cytokine levels in cell-free supernatants of MDM ( a ) and ( b ) iMg cultures were measured with Bio-Plex Pro 48-Plex human cytokine screening panel. Heatmaps show log 2 fold changes from the HIV + vehicle condition. Supernatants were pooled from 4 wells/condition at days postinfection 9 (DPI 9) and assayed in duplicate. n = 6 MDM donors and 3 iMg lines. Student’s t test, ** p < 0.01. c. Levels of cytokines reportedly associated with HIV-induced brain injury in MDM and iMg supernatants, measured with Bio-Plex Pro 48-Plex human cytokine screening panel. d , e. Levels of tumor necrosis factor alpha (TNF-α) and IL-6 in ( d ) MDM and ( e ) iMg culture supernatants, measured with AlphaLISA. n = 6 MDM donors and 3 iMg lines. Student’s t test, * p < 0.05

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: Infection

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: RNA-seq identifies convergent and divergent responses to CB 2 signaling between HIV-infected MDMs and iMg. a , b . Top 10 GO biological process gene sets enriched in the significant differentially expressed genes (DEGs) in comparison between HIV-infected/vehicle-treated and HIV-infected/JWH-133 treated ( a ) MDMs and ( b ) iMg. c. Top 10 KEGG gene sets enriched in the significant DEGs in comparison between HIV-infected/JWH-133-treated MDMs and iMg. n = 2 MDM donors and 2 iMg lines

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: RNA Sequencing, Infection, Comparison

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: CB 2 activation has minimal impact on transcriptional priming of the NLRP3 inflammasome in MDMs and iMg with established HIV infection. (a) NLRP3 inflammasome gene expression in MDMs on DPI 9, determined with qRT-PCR, represented as log 2 fold change from Mock + Veh. n = 3 MDM donors. (b) NLRP3 inflammasome gene expression in iMg on DPI 9, determined with bulk RNA-seq. n = 3 iMg lines

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: Activation Assay, Infection, Gene Expression, Quantitative RT-PCR, RNA Sequencing

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: In iMg, CB 2 -specific agonists impair HIV-induced NLRP3 inflammasome activation with a corresponding increase in autophagosome number. (a) Quantification of the number of NLRP3 + /ASC + specks in iMg detected with immunostaining. Average of 2 wells/condition from 3 differentiations in 2 iMg lines, one-way ANOVA with Dunnett’s correction for multiple comparisons. * p < 0.05. (b) Autophagic vacuoles measured by an autophagy assay. n = 3 iMg lines, one-way ANOVA with Dunnett’s correction for multiple comparisons, * p < 0.05. (c) Caspase 1 activity measured by the Caspase-Glo 1 inflammasome assay, with results normalized to HIV + Veh. n = 3 iMg lines, one-way ANOVA with Dunnett’s correction for multiple comparisons, * p < 0.05. (d) Levels of IL-1β in iMg supernatants, measured by AlphaLISA. n = 3 iMg lines, one-way ANOVA with Dunnett’s correction for multiple comparisons, ** p < 0.01; ns, not significant

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: Activation Assay, Immunostaining, Activity Assay

Journal: bioRxiv

Article Title: Roadmap For The Expression Of Canonical and Extended Endocannabinoid System Receptors and Proteins in Peripheral Organs of Preclinical Animal Models

doi: 10.1101/2023.06.10.544455

Figure Lengend Snippet: List of primers used to determine the relative expression of canonical and extended endocannabinoid receptors in mice ( Mus musculus ).

Article Snippet: htr1a , Rn01637601_m1 , ThermoFisher Scientific.

Techniques: Expressing, Mouse Assay

Journal: bioRxiv

Article Title: Roadmap For The Expression Of Canonical and Extended Endocannabinoid System Receptors and Proteins in Peripheral Organs of Preclinical Animal Models

doi: 10.1101/2023.06.10.544455

Figure Lengend Snippet: List of primers used to determine the relative expression of canonical and extended endocannabinoid receptors in Rhesus macaques ( Macaca mulatta ).

Article Snippet: htr1a , Rn01637601_m1 , ThermoFisher Scientific.

Techniques: Expressing

Journal: bioRxiv

Article Title: Roadmap For The Expression Of Canonical and Extended Endocannabinoid System Receptors and Proteins in Peripheral Organs of Preclinical Animal Models

doi: 10.1101/2023.06.10.544455

Figure Lengend Snippet: Relative expression of cnr1 and cnr2 was determined using qPCR from the colon, heart, kidney, liver, MLN, spleen, and visceral fat from mice, rats and NHP. A) C nr1 mRNA in mice was detected in the colon, kidney (4/5 mice), spleen, and visceral fat, having the highest levels in the latter. B) C nr1 was detected in partial samples of the rat model colon (4/6 rats), heart (1/6 rats), kidney, and spleen (1/6 rats), having statistically higher levels of expression in the kidney when compared to the heart, liver, MLN, and spleen. C) In NHP, cnr1 mRNA was detected in the spleen (2/3 rhesus) and visceral fat at comparable levels. D) Cnr2 was detected only in the spleen and visceral fat (4/5 mice), at comparable levels. The spleen had statistically significantly higher levels as compared to the colon, heart, kidney, and liver. E) Cnr2 was detected in the colon (2/4 rats), heart (1/6 rats), MLN (4/6 rats) and spleen of rats. F) In NHP, cnr2 mRNA was detected in the liver (1/4 rhesus), MLN and spleen. Detection levels were significantly higher in the spleen when compared to the heart, kidney, and liver, but with significant differences when compared to the liver (1/4 rhesus), kidney, and heart. Data are represented as the mean ± SEM. (*p-value >0.05, **p-value>0.001, ***p-value>0.0001, ****p-value>0.00001).

Article Snippet: htr1a , Rn01637601_m1 , ThermoFisher Scientific.

Techniques: Expressing

Journal: bioRxiv

Article Title: Roadmap For The Expression Of Canonical and Extended Endocannabinoid System Receptors and Proteins in Peripheral Organs of Preclinical Animal Models

doi: 10.1101/2023.06.10.544455

Figure Lengend Snippet: Summary of the findings of the expression of the canonical and extended ECS in mice ( Mus musculus ).

Article Snippet: htr1a , Rn01637601_m1 , ThermoFisher Scientific.

Techniques: Expressing, Mouse Assay

Journal: bioRxiv

Article Title: Roadmap For The Expression Of Canonical and Extended Endocannabinoid System Receptors and Proteins in Peripheral Organs of Preclinical Animal Models

doi: 10.1101/2023.06.10.544455

Figure Lengend Snippet: Summary of the findings of the expression of the canonical and extended ECS in Rhesus macaques ( Macaca mulatta ).

Article Snippet: htr1a , Rn01637601_m1 , ThermoFisher Scientific.

Techniques: Expressing

Journal: bioRxiv

Article Title: Roadmap For The Expression Of Canonical and Extended Endocannabinoid System Receptors and Proteins in Peripheral Organs of Preclinical Animal Models

doi: 10.1101/2023.06.10.544455

Figure Lengend Snippet: List of primers used to determine the relative expression of canonical and extended endocannabinoid receptors in rats ( Ratus norvegicus ).

Article Snippet: htr1a , Rn01637601_m1 , ThermoFisher Scientific.

Techniques: Expressing

Journal: bioRxiv

Article Title: Roadmap For The Expression Of Canonical and Extended Endocannabinoid System Receptors and Proteins in Peripheral Organs of Preclinical Animal Models

doi: 10.1101/2023.06.10.544455

Figure Lengend Snippet: Summary of the findings of the expression of the canonical and extended ECS in rats ( Ratus norvegicus ).

Article Snippet: htr1a , Rn01637601_m1 , ThermoFisher Scientific.

Techniques: Expressing

Journal: Nanomaterials

Article Title: ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure

doi: 10.3390/nano11092319

Figure Lengend Snippet: Effect of ZnONPs on aorta contractility. ( a ) Effect of ACPA (CB 1 receptor agonist) on contraction in aortic rings in different experimental groups. ( b ) Effect of HU308 (CB 2 receptor agonist) on contraction in aortic rings in different experimental groups. Blue bars correspond to the control non-treated group, green bars correspond to the ZnONPs treated-groups at different times; ( a ) there is a significant difference compared to the phenylephrine group with p < 0.05; ( b ) there is a significant difference compared to the control group with p < 0.05.

Article Snippet: Next, aorta sections were blocked (Animal-free blocker and diluent, Vector laboratories Inc., Burlingame, CA, USA) and incubated with rabbit polyclonal Anti-CB 1 (1:100, Alomone, Labs, Jerusalem, Israel) or rabbit polyclonal Anti-CB 2 (1:100, Alomone, Labs, Jerusalem, Israel) primary antibody and co-labeled with mouse monoclonal Anti-alpha smooth muscle Actin antibody (1:200, Abcam, Cambrige, UK) overnight.

Techniques:

Journal: Nanomaterials

Article Title: ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure

doi: 10.3390/nano11092319

Figure Lengend Snippet: Effect of ZnONPs on the CB 1 and CB 2 receptors expression in the aorta wall. ( a ) CB 1 expression, ( b ) CB 2 expression. Blue bars correspond to the control non-treated group, green bars correspond to the ZnONPs treated-groups at different times; ( a ) there is a significant difference compared to the control group with p < 0.05.

Article Snippet: Next, aorta sections were blocked (Animal-free blocker and diluent, Vector laboratories Inc., Burlingame, CA, USA) and incubated with rabbit polyclonal Anti-CB 1 (1:100, Alomone, Labs, Jerusalem, Israel) or rabbit polyclonal Anti-CB 2 (1:100, Alomone, Labs, Jerusalem, Israel) primary antibody and co-labeled with mouse monoclonal Anti-alpha smooth muscle Actin antibody (1:200, Abcam, Cambrige, UK) overnight.

Techniques: Expressing

Journal: Nanomaterials

Article Title: ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure

doi: 10.3390/nano11092319

Figure Lengend Snippet: Representative images showed ZnONPs effect on CB 1 and CB 2 receptors expression in aorta wall. Transmitted light images (grey). Scale bar corresponds to 50 μm. Smooth muscle α-actin was detected with a specific antibody labeled with Alexa Fluor 568 (red). CB 1 and CB 2 located on the aorta ring were detected by specific antibodies and labeled with FITC (green). Image overlap indicates a high degree of colocalization of CB 1 or CB 2, and smooth muscle α-actin (yellow). Nuclei were counterstained with DAPI dye (blue).

Article Snippet: Next, aorta sections were blocked (Animal-free blocker and diluent, Vector laboratories Inc., Burlingame, CA, USA) and incubated with rabbit polyclonal Anti-CB 1 (1:100, Alomone, Labs, Jerusalem, Israel) or rabbit polyclonal Anti-CB 2 (1:100, Alomone, Labs, Jerusalem, Israel) primary antibody and co-labeled with mouse monoclonal Anti-alpha smooth muscle Actin antibody (1:200, Abcam, Cambrige, UK) overnight.

Techniques: Expressing, Labeling