cn03 Search Results


rho activator ii  (Cytoskeleton Inc)
96
Cytoskeleton Inc rho activator ii
Rho Activator Ii, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cn03/Rho+activator+II/pm39266508-309-30-33
Average 96 stars, based on 1 article reviews
rho activator ii - by Bioz Stars, 2026-11
96/100 stars
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cn03  (VANGL2 LTD)
90
VANGL2 LTD cn03
VANGL2 regulates MLC2 activation and force generation via RhoA signaling. Representative images showing wildtype (A) and Vangl2 Lp/+ (B) TECs stained with pMLC2 (green) and phalloidin (magenta). Nuclei are shown in gray (DAPI). (C) Quantification of pMLC2 relative fluorescence intensity (RFI) in Vangl2 Lp/+ relative to wildtype (presented as mean ± SEM). n = 3 independent experiments; TECs from three mice were pooled for each group per experiment; two technical replicates for each group per experiment; at least three fields were taken per technical replicate. Mann–Whitney U -test, * p < 0.05. (D) Schematic representation of the elastic micropillars used to measure cellular traction forces. Cells were seeded on a rat-tail collagen I coated array of micropillars and incubated at 37°C with 5% CO 2 for 24 h. Individual pillar deflection from its original position in the array (Δx) is tracked via time-lapse microscopy and converted to traction force based on the pillar spring constant ( k = 1.36 nN/μm). Heat maps of the traction forces distribution in a representative wildtype (E) and Vangl2 Lp/+ (F) TEC. Cell boundary is outlined in white. (G) Quantification of the mean maximum traction forces exerted by wildtype ( n = 75 cells) and Vangl2 Lp/+ TECs ( n = 92 cells) on the micropillars (presented as mean ± SEM). n = 2 independent experiments; in each experiment, tracheas from three separate mice were pooled for each group; two technical replicates for each group per experiment; each dot represents mean maximum traction force per cell. Two-tailed unpaired Student’s t -test, *** p < 0.001. Representative images showing Vangl2 Lp/+ TECs without treatment (H) and Vangl2 Lp/+ TECs treated with Rho activator, <t>CN03</t> (I) . TECs were stained with pMLC2 (green) and phalloidin (magenta). Nuclei were stained with DAPI (gray). n = 3 independent experiments; TECs from three mice were pooled for each group per experiment; two technical replicates for each group per experiment.
Cn03, supplied by VANGL2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cn03/cn03/pmc07658195-94-4-14
Average 90 stars, based on 1 article reviews
cn03 - by Bioz Stars, 2026-11
90/100 stars
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single 20 mg/kg dose of either cn03 or lp-cn03  (WIL Research)
90
WIL Research single 20 mg/kg dose of either cn03 or lp-cn03
VANGL2 regulates MLC2 activation and force generation via RhoA signaling. Representative images showing wildtype (A) and Vangl2 Lp/+ (B) TECs stained with pMLC2 (green) and phalloidin (magenta). Nuclei are shown in gray (DAPI). (C) Quantification of pMLC2 relative fluorescence intensity (RFI) in Vangl2 Lp/+ relative to wildtype (presented as mean ± SEM). n = 3 independent experiments; TECs from three mice were pooled for each group per experiment; two technical replicates for each group per experiment; at least three fields were taken per technical replicate. Mann–Whitney U -test, * p < 0.05. (D) Schematic representation of the elastic micropillars used to measure cellular traction forces. Cells were seeded on a rat-tail collagen I coated array of micropillars and incubated at 37°C with 5% CO 2 for 24 h. Individual pillar deflection from its original position in the array (Δx) is tracked via time-lapse microscopy and converted to traction force based on the pillar spring constant ( k = 1.36 nN/μm). Heat maps of the traction forces distribution in a representative wildtype (E) and Vangl2 Lp/+ (F) TEC. Cell boundary is outlined in white. (G) Quantification of the mean maximum traction forces exerted by wildtype ( n = 75 cells) and Vangl2 Lp/+ TECs ( n = 92 cells) on the micropillars (presented as mean ± SEM). n = 2 independent experiments; in each experiment, tracheas from three separate mice were pooled for each group; two technical replicates for each group per experiment; each dot represents mean maximum traction force per cell. Two-tailed unpaired Student’s t -test, *** p < 0.001. Representative images showing Vangl2 Lp/+ TECs without treatment (H) and Vangl2 Lp/+ TECs treated with Rho activator, <t>CN03</t> (I) . TECs were stained with pMLC2 (green) and phalloidin (magenta). Nuclei were stained with DAPI (gray). n = 3 independent experiments; TECs from three mice were pooled for each group per experiment; two technical replicates for each group per experiment.
Single 20 Mg/Kg Dose Of Either Cn03 Or Lp Cn03, supplied by WIL Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cn03/single+20+mg+kg+dose+of+either+cn03+or+lp+cn03/pmc05879685__pnas__201718792SI-248-18-6
Average 90 stars, based on 1 article reviews
single 20 mg/kg dose of either cn03 or lp-cn03 - by Bioz Stars, 2026-11
90/100 stars
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dithio-cn03  (TargetMol)
N/A
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Image Search Results


VANGL2 regulates MLC2 activation and force generation via RhoA signaling. Representative images showing wildtype (A) and Vangl2 Lp/+ (B) TECs stained with pMLC2 (green) and phalloidin (magenta). Nuclei are shown in gray (DAPI). (C) Quantification of pMLC2 relative fluorescence intensity (RFI) in Vangl2 Lp/+ relative to wildtype (presented as mean ± SEM). n = 3 independent experiments; TECs from three mice were pooled for each group per experiment; two technical replicates for each group per experiment; at least three fields were taken per technical replicate. Mann–Whitney U -test, * p < 0.05. (D) Schematic representation of the elastic micropillars used to measure cellular traction forces. Cells were seeded on a rat-tail collagen I coated array of micropillars and incubated at 37°C with 5% CO 2 for 24 h. Individual pillar deflection from its original position in the array (Δx) is tracked via time-lapse microscopy and converted to traction force based on the pillar spring constant ( k = 1.36 nN/μm). Heat maps of the traction forces distribution in a representative wildtype (E) and Vangl2 Lp/+ (F) TEC. Cell boundary is outlined in white. (G) Quantification of the mean maximum traction forces exerted by wildtype ( n = 75 cells) and Vangl2 Lp/+ TECs ( n = 92 cells) on the micropillars (presented as mean ± SEM). n = 2 independent experiments; in each experiment, tracheas from three separate mice were pooled for each group; two technical replicates for each group per experiment; each dot represents mean maximum traction force per cell. Two-tailed unpaired Student’s t -test, *** p < 0.001. Representative images showing Vangl2 Lp/+ TECs without treatment (H) and Vangl2 Lp/+ TECs treated with Rho activator, CN03 (I) . TECs were stained with pMLC2 (green) and phalloidin (magenta). Nuclei were stained with DAPI (gray). n = 3 independent experiments; TECs from three mice were pooled for each group per experiment; two technical replicates for each group per experiment.

Journal: Frontiers in Cell and Developmental Biology

Article Title: The Planar Polarity Component VANGL2 Is a Key Regulator of Mechanosignaling

doi: 10.3389/fcell.2020.577201

Figure Lengend Snippet: VANGL2 regulates MLC2 activation and force generation via RhoA signaling. Representative images showing wildtype (A) and Vangl2 Lp/+ (B) TECs stained with pMLC2 (green) and phalloidin (magenta). Nuclei are shown in gray (DAPI). (C) Quantification of pMLC2 relative fluorescence intensity (RFI) in Vangl2 Lp/+ relative to wildtype (presented as mean ± SEM). n = 3 independent experiments; TECs from three mice were pooled for each group per experiment; two technical replicates for each group per experiment; at least three fields were taken per technical replicate. Mann–Whitney U -test, * p < 0.05. (D) Schematic representation of the elastic micropillars used to measure cellular traction forces. Cells were seeded on a rat-tail collagen I coated array of micropillars and incubated at 37°C with 5% CO 2 for 24 h. Individual pillar deflection from its original position in the array (Δx) is tracked via time-lapse microscopy and converted to traction force based on the pillar spring constant ( k = 1.36 nN/μm). Heat maps of the traction forces distribution in a representative wildtype (E) and Vangl2 Lp/+ (F) TEC. Cell boundary is outlined in white. (G) Quantification of the mean maximum traction forces exerted by wildtype ( n = 75 cells) and Vangl2 Lp/+ TECs ( n = 92 cells) on the micropillars (presented as mean ± SEM). n = 2 independent experiments; in each experiment, tracheas from three separate mice were pooled for each group; two technical replicates for each group per experiment; each dot represents mean maximum traction force per cell. Two-tailed unpaired Student’s t -test, *** p < 0.001. Representative images showing Vangl2 Lp/+ TECs without treatment (H) and Vangl2 Lp/+ TECs treated with Rho activator, CN03 (I) . TECs were stained with pMLC2 (green) and phalloidin (magenta). Nuclei were stained with DAPI (gray). n = 3 independent experiments; TECs from three mice were pooled for each group per experiment; two technical replicates for each group per experiment.

Article Snippet: Confocal images showed that CN03 treatment led to striking changes in actomyosin organization in Vangl2 Lp/+ TECs.

Techniques: Activation Assay, Staining, Fluorescence, MANN-WHITNEY, Incubation, Time-lapse Microscopy, Two Tailed Test