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MedChemExpress cmc na
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Selleck Chemicals p12
Figure 1. PLX5622 administration effectively removed microglia in the retina. (A) Natal mice were placed in an oxygen box with 75% (±2%) from P7 to <t>P12</t> to establish the OIR model, then were switched to room air at P12. They were subjected to oral administration of PLX5622 from P12 to P25. (B) The weights of mice in the two groups were tested each day (n = 3 per group). (C) Retina samples were collected from the OIR and Plx groups on P17. The expression of CX3CR1 (green, GFP) on MG was analyzed by FACs (n = 3 per group). (D) mRNA expression of microglia (TMEM119), M1 subtype-related genes (iNOS, CD86), and M2 subtype-related genes (CD163) were determined by qRT-PCR (n = 3 per group). (E) Immunofluorescence staining of CX3CR1 (GFP, green) in the retinas of CX3CR1-GFP mice were collected on P12, 14, 17, 19, and 25 (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *** p < 0.001; **** p < 0.0001; NS, no significance.
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Bruker Corporation bruker cmc
Figure 1. PLX5622 administration effectively removed microglia in the retina. (A) Natal mice were placed in an oxygen box with 75% (±2%) from P7 to <t>P12</t> to establish the OIR model, then were switched to room air at P12. They were subjected to oral administration of PLX5622 from P12 to P25. (B) The weights of mice in the two groups were tested each day (n = 3 per group). (C) Retina samples were collected from the OIR and Plx groups on P17. The expression of CX3CR1 (green, GFP) on MG was analyzed by FACs (n = 3 per group). (D) mRNA expression of microglia (TMEM119), M1 subtype-related genes (iNOS, CD86), and M2 subtype-related genes (CD163) were determined by qRT-PCR (n = 3 per group). (E) Immunofluorescence staining of CX3CR1 (GFP, green) in the retinas of CX3CR1-GFP mice were collected on P12, 14, 17, 19, and 25 (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *** p < 0.001; **** p < 0.0001; NS, no significance.
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MedChemExpress cat no cmc na
Figure 1. PLX5622 administration effectively removed microglia in the retina. (A) Natal mice were placed in an oxygen box with 75% (±2%) from P7 to <t>P12</t> to establish the OIR model, then were switched to room air at P12. They were subjected to oral administration of PLX5622 from P12 to P25. (B) The weights of mice in the two groups were tested each day (n = 3 per group). (C) Retina samples were collected from the OIR and Plx groups on P17. The expression of CX3CR1 (green, GFP) on MG was analyzed by FACs (n = 3 per group). (D) mRNA expression of microglia (TMEM119), M1 subtype-related genes (iNOS, CD86), and M2 subtype-related genes (CD163) were determined by qRT-PCR (n = 3 per group). (E) Immunofluorescence staining of CX3CR1 (GFP, green) in the retinas of CX3CR1-GFP mice were collected on P12, 14, 17, 19, and 25 (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *** p < 0.001; **** p < 0.0001; NS, no significance.
Cat No Cmc Na, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MedChemExpress cmc na solution
Figure 1. PLX5622 administration effectively removed microglia in the retina. (A) Natal mice were placed in an oxygen box with 75% (±2%) from P7 to <t>P12</t> to establish the OIR model, then were switched to room air at P12. They were subjected to oral administration of PLX5622 from P12 to P25. (B) The weights of mice in the two groups were tested each day (n = 3 per group). (C) Retina samples were collected from the OIR and Plx groups on P17. The expression of CX3CR1 (green, GFP) on MG was analyzed by FACs (n = 3 per group). (D) mRNA expression of microglia (TMEM119), M1 subtype-related genes (iNOS, CD86), and M2 subtype-related genes (CD163) were determined by qRT-PCR (n = 3 per group). (E) Immunofluorescence staining of CX3CR1 (GFP, green) in the retinas of CX3CR1-GFP mice were collected on P12, 14, 17, 19, and 25 (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *** p < 0.001; **** p < 0.0001; NS, no significance.
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Nanografi Advanced Materials carboxymethyl cellulose
Figure 1. PLX5622 administration effectively removed microglia in the retina. (A) Natal mice were placed in an oxygen box with 75% (±2%) from P7 to <t>P12</t> to establish the OIR model, then were switched to room air at P12. They were subjected to oral administration of PLX5622 from P12 to P25. (B) The weights of mice in the two groups were tested each day (n = 3 per group). (C) Retina samples were collected from the OIR and Plx groups on P17. The expression of CX3CR1 (green, GFP) on MG was analyzed by FACs (n = 3 per group). (D) mRNA expression of microglia (TMEM119), M1 subtype-related genes (iNOS, CD86), and M2 subtype-related genes (CD163) were determined by qRT-PCR (n = 3 per group). (E) Immunofluorescence staining of CX3CR1 (GFP, green) in the retinas of CX3CR1-GFP mice were collected on P12, 14, 17, 19, and 25 (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *** p < 0.001; **** p < 0.0001; NS, no significance.
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Bruker Corporation cmc q software
Figure 1. PLX5622 administration effectively removed microglia in the retina. (A) Natal mice were placed in an oxygen box with 75% (±2%) from P7 to <t>P12</t> to establish the OIR model, then were switched to room air at P12. They were subjected to oral administration of PLX5622 from P12 to P25. (B) The weights of mice in the two groups were tested each day (n = 3 per group). (C) Retina samples were collected from the OIR and Plx groups on P17. The expression of CX3CR1 (green, GFP) on MG was analyzed by FACs (n = 3 per group). (D) mRNA expression of microglia (TMEM119), M1 subtype-related genes (iNOS, CD86), and M2 subtype-related genes (CD163) were determined by qRT-PCR (n = 3 per group). (E) Immunofluorescence staining of CX3CR1 (GFP, green) in the retinas of CX3CR1-GFP mice were collected on P12, 14, 17, 19, and 25 (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *** p < 0.001; **** p < 0.0001; NS, no significance.
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MedChemExpress sodium carboxymethylcellulose
Figure 1. PLX5622 administration effectively removed microglia in the retina. (A) Natal mice were placed in an oxygen box with 75% (±2%) from P7 to <t>P12</t> to establish the OIR model, then were switched to room air at P12. They were subjected to oral administration of PLX5622 from P12 to P25. (B) The weights of mice in the two groups were tested each day (n = 3 per group). (C) Retina samples were collected from the OIR and Plx groups on P17. The expression of CX3CR1 (green, GFP) on MG was analyzed by FACs (n = 3 per group). (D) mRNA expression of microglia (TMEM119), M1 subtype-related genes (iNOS, CD86), and M2 subtype-related genes (CD163) were determined by qRT-PCR (n = 3 per group). (E) Immunofluorescence staining of CX3CR1 (GFP, green) in the retinas of CX3CR1-GFP mice were collected on P12, 14, 17, 19, and 25 (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *** p < 0.001; **** p < 0.0001; NS, no significance.
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Cerilliant Corporation meprobamate
Figure 1. PLX5622 administration effectively removed microglia in the retina. (A) Natal mice were placed in an oxygen box with 75% (±2%) from P7 to <t>P12</t> to establish the OIR model, then were switched to room air at P12. They were subjected to oral administration of PLX5622 from P12 to P25. (B) The weights of mice in the two groups were tested each day (n = 3 per group). (C) Retina samples were collected from the OIR and Plx groups on P17. The expression of CX3CR1 (green, GFP) on MG was analyzed by FACs (n = 3 per group). (D) mRNA expression of microglia (TMEM119), M1 subtype-related genes (iNOS, CD86), and M2 subtype-related genes (CD163) were determined by qRT-PCR (n = 3 per group). (E) Immunofluorescence staining of CX3CR1 (GFP, green) in the retinas of CX3CR1-GFP mice were collected on P12, 14, 17, 19, and 25 (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *** p < 0.001; **** p < 0.0001; NS, no significance.
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Image Search Results


Figure 1. PLX5622 administration effectively removed microglia in the retina. (A) Natal mice were placed in an oxygen box with 75% (±2%) from P7 to P12 to establish the OIR model, then were switched to room air at P12. They were subjected to oral administration of PLX5622 from P12 to P25. (B) The weights of mice in the two groups were tested each day (n = 3 per group). (C) Retina samples were collected from the OIR and Plx groups on P17. The expression of CX3CR1 (green, GFP) on MG was analyzed by FACs (n = 3 per group). (D) mRNA expression of microglia (TMEM119), M1 subtype-related genes (iNOS, CD86), and M2 subtype-related genes (CD163) were determined by qRT-PCR (n = 3 per group). (E) Immunofluorescence staining of CX3CR1 (GFP, green) in the retinas of CX3CR1-GFP mice were collected on P12, 14, 17, 19, and 25 (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *** p < 0.001; **** p < 0.0001; NS, no significance.

Journal: Life (Basel, Switzerland)

Article Title: Distinguished Functions of Microglia in the Two Stages of Oxygen-Induced Retinopathy: A Novel Target in the Treatment of Ischemic Retinopathy.

doi: 10.3390/life12101676

Figure Lengend Snippet: Figure 1. PLX5622 administration effectively removed microglia in the retina. (A) Natal mice were placed in an oxygen box with 75% (±2%) from P7 to P12 to establish the OIR model, then were switched to room air at P12. They were subjected to oral administration of PLX5622 from P12 to P25. (B) The weights of mice in the two groups were tested each day (n = 3 per group). (C) Retina samples were collected from the OIR and Plx groups on P17. The expression of CX3CR1 (green, GFP) on MG was analyzed by FACs (n = 3 per group). (D) mRNA expression of microglia (TMEM119), M1 subtype-related genes (iNOS, CD86), and M2 subtype-related genes (CD163) were determined by qRT-PCR (n = 3 per group). (E) Immunofluorescence staining of CX3CR1 (GFP, green) in the retinas of CX3CR1-GFP mice were collected on P12, 14, 17, 19, and 25 (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *** p < 0.001; **** p < 0.0001; NS, no significance.

Article Snippet: From P12, when the mice were taken back to room air, the natal mice were treated daily with oral gavage with 100 μL of solution (10 mg/mL) in CMC-Na (Selleck, China) per 10 g of body weight (at the dose of 100 mg/kg body weight).

Techniques: Expressing, Quantitative RT-PCR, Staining