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Image Search Results
Journal: Life (Basel, Switzerland)
Article Title: Distinguished Functions of Microglia in the Two Stages of Oxygen-Induced Retinopathy: A Novel Target in the Treatment of Ischemic Retinopathy.
doi: 10.3390/life12101676
Figure Lengend Snippet: Figure 1. PLX5622 administration effectively removed microglia in the retina. (A) Natal mice were placed in an oxygen box with 75% (±2%) from P7 to P12 to establish the OIR model, then were switched to room air at P12. They were subjected to oral administration of PLX5622 from P12 to P25. (B) The weights of mice in the two groups were tested each day (n = 3 per group). (C) Retina samples were collected from the OIR and Plx groups on P17. The expression of CX3CR1 (green, GFP) on MG was analyzed by FACs (n = 3 per group). (D) mRNA expression of microglia (TMEM119), M1 subtype-related genes (iNOS, CD86), and M2 subtype-related genes (CD163) were determined by qRT-PCR (n = 3 per group). (E) Immunofluorescence staining of CX3CR1 (GFP, green) in the retinas of CX3CR1-GFP mice were collected on P12, 14, 17, 19, and 25 (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *** p < 0.001; **** p < 0.0001; NS, no significance.
Article Snippet: From
Techniques: Expressing, Quantitative RT-PCR, Staining