clustergrams Search Results


clustergram module  (Shotgun Proteomics company)
90
Shotgun Proteomics company clustergram module
Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the <t>Clustergram</t> module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.
Clustergram Module, supplied by Shotgun Proteomics company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clustergram  (Qiagen)
90
Qiagen clustergram
Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the <t>Clustergram</t> module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.
Clustergram, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clustergram/product/Qiagen
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clustergram - by Bioz Stars, 2026-03
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clustergram plot  (Qiagen)
90
Qiagen clustergram plot
Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the <t>Clustergram</t> module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.
Clustergram Plot, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clustergram plot - by Bioz Stars, 2026-03
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dash-bio.clustergram package  (Plotly Technologies Inc)
90
Plotly Technologies Inc dash-bio.clustergram package
Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the <t>Clustergram</t> module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.
Dash Bio.Clustergram Package, supplied by Plotly Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dash-bio.clustergram package - by Bioz Stars, 2026-03
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clustergram function in pls toolbox 7.3.1  (Eigenvector Research Inc)
90
Eigenvector Research Inc clustergram function in pls toolbox 7.3.1
Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the <t>Clustergram</t> module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.
Clustergram Function In Pls Toolbox 7.3.1, supplied by Eigenvector Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clustergram representation  (Verlag GmbH)
90
Verlag GmbH clustergram representation
Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the <t>Clustergram</t> module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.
Clustergram Representation, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clustergram representation/product/Verlag GmbH
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clustergram representation - by Bioz Stars, 2026-03
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clustergram  (Wolters Kluwer Health)
90
Wolters Kluwer Health clustergram
Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the <t>Clustergram</t> module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.
Clustergram, supplied by Wolters Kluwer Health, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clustergram/product/Wolters Kluwer Health
Average 90 stars, based on 1 article reviews
clustergram - by Bioz Stars, 2026-03
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Image Search Results


Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the Clustergram module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.

Journal: Scientific Reports

Article Title: Mitofusin 1 silencing decreases the senescent associated secretory phenotype, promotes immune cell recruitment and delays melanoma tumor growth after chemotherapy

doi: 10.1038/s41598-024-51427-7

Figure Lengend Snippet: Mfn1 silencing in melanoma cells affects the senescence associated secretory phenotype (SASP). B16-F1 cells were transduced with lentiviral particles carrying shScr or shMfn1 and selected with puromycin. ( a ) Mfn1 expression was determined by RT-qPCR and reported relative to control value. Unpaired t-test, two-tails, ***P < 0.0001. ( b ) Representative images of mitofusin 1 (MFN1) protein levels, assessed by immunocytochemistry. Unpaired t-test, two-tails, ***P < 0.0001 (n = 3). ( c ) ShScr and shMfn1 cells were then exposed twice to TMZ (200 μM) or the vehicle DMSO for five hours with a 24 h interval. Samples were analyzed five days after the last exposure to the drug. A schematic representation of the different groups obtained after transduction and treatment with TMZ or vehicle is shown. ( d ) mRNA levels of Cdkn1a (p21). Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P = 0.67) and their interaction (P = 0.41). Tukey post-hoc for multiple comparisons, different letters are significantly different (P ≤ 0.0008). ( e ) Number of live cells in the culture assessed with Trypan blue at different time points after the last treatment. Two-way ANOVA and Tukey post-hoc for multiple comparisons, ***P < 0.0001 ShScr TMZ vs ShScr DMSO, and ShMfn1TMZ vs ShScr DMSO. ( f ) SA-β-galactosidase activity. The percentage of positive cells is shown. Two-way ANOVA, main effects of treatment (P < 0.0001), shRNA (P < 0.0001) and their interaction (P < 0.0001). Tukey post-hoc for multiple comparisons, different letters are significantly different (P < 0. 0001). In ( a , b , d , e , f ) results are the mean ± SD (n = 3–4). ( g ) Conditioned media was obtained as described in Methods. Protein content was analyzed by shotgun proteomics, and a heatmap generated with the Clustergram module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.

Article Snippet: Protein content was analyzed by shotgun proteomics, and a heatmap generated with the Clustergram module from PatternLab for Proteomics software. ( h ) Principal components analysis (PCA) was performed with the Buzios module from PatternLab for Proteomics software.

Techniques: Transduction, Expressing, Quantitative RT-PCR, Control, Immunocytochemistry, shRNA, Activity Assay, Generated, Software