Journal: Biomaterials

Article Title: Genomic Form of Rhodopsin DNA Nanoparticles Rescued Autosomal Dominant Retinitis Pigmentosa in the P23H Knock-in Mouse Model

doi: 10.1016/j.biomaterials.2017.12.004

Figure Lengend Snippet: Subcellular localization of Rho proteins in Cos-7 cells, encoded by pCAGIG-sgRho-IRES-EGFP and pCAGIG-co-sgRho-IRES-EGFP plasmid DNA, were determined by CLSM imaging. Untransfected cells (top panel) were used as a negative control. Calreticulin (white) is an ER marker. The Rho protein (red) was not retained in the ER. Rho protein was expressed throughout the cells in both gene transfections. EGFP expression (green) was used as a transfection control. The nucleus is stained with DAPI. The CLSM images were acquired at 40× magnification using a Zeiss LSM 710. Scale bar = 10 μm.

Article Snippet: The CLSM images were acquired at 40× magnification using a Zeiss LSM 710.

Techniques: Plasmid Preparation, Imaging, Negative Control, Marker, Transfection, Expressing, Control, Staining

Journal: Biomaterials

Article Title: Genomic Form of Rhodopsin DNA Nanoparticles Rescued Autosomal Dominant Retinitis Pigmentosa in the P23H Knock-in Mouse Model

doi: 10.1016/j.biomaterials.2017.12.004

Figure Lengend Snippet: Evaluation of Rho protein expression in the PRs of RhoP23H/P23H retina at PI-60 days. Rabbit anti-Rho and mouse anti-alpha Na+/K+ ATPase were used to detect Rho (Red, AF-555) and plasma membrane (green, AF-488) respectively. The images were acquired at 40× magnification using a Zeiss LSM 710 CLSM. The formation of small inner and outer segments in the retina are indicated by white arrows. OS: outer segment; IS: inner segment; ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer. Scale bar = 5 μm.

Article Snippet: The CLSM images were acquired at 40× magnification using a Zeiss LSM 710.

Techniques: Expressing, Clinical Proteomics, Membrane

Journal:

Article Title: Immunogold Electron Microscopic Demonstration of Distinct Submembranous Localization of the Activated ?PKC Depending on the Stimulation

doi: 10.1369/jhc.7A7291.2007

Figure Lengend Snippet: Plasma membrane translocation of γPKC–GFP induced by TPA, A23187, UTP, or NMDA was followed by confocal laser-scanning microscopy. TPA treatment induced the slow and irreversible translocation of γPKC–GFP from the cytoplasm to the plasma membrane (top row). A23187 treatment induced the rapid and reversible translocation from the cytoplasm to the plasma membrane (second row). UTP induced the rapid and reversible translocation (third row). NMDA treatment to the CHO-K1 cells coexpressing NMDA receptors induced the rapid and reversible translocation of γPKC–GFP from the cytoplasm to the plasma membrane (fourth row). Bar = 10 μm.

Article Snippet: Fluorescence of GFP was monitored by fluorescent CLSM (Carl Zeiss; Jena, Germany) using 488-nm argon excitation with a 515-nm-long-pass barrier filter.

Techniques: Translocation Assay, Confocal Laser Scanning Microscopy