Journal: Oncotarget

Article Title: Enhancement of TRAIL-induced apoptosis by 5-fluorouracil requires activating Bax and p53 pathways in TRAIL-resistant lung cancers

doi: 10.18632/oncotarget.14994

Figure Lengend Snippet: A549 adenocarcinoma cells were pretreated with 5-fluorouracil at different concentrations (0, 10, 20, and 40 μM) for 12 h. A and B . Cells were harvested and analyzed by Western blotting to determine the expression levels of DR-4, DR-5, Ac-cas3 and Ac-cas8; C . Representative immunocytochemistry was implemented in A549 cells after co-treatment with 5-fluorouracil (12 h) and 200 ng/ml TRAIL (1 h); D . Ac-cas3 and Ac-cas8 expression levels determined by western blot analysis. β-actin was used as loading control. 5-Flu: 5-Fluorouracil; Ac-cas3: Activated caspase 3; Ac-cas8: Activated caspase 8; TRAIL: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand.

Article Snippet: Immunoblotting was performed using antibodies against cleaved caspase-3 (Cell Signaling Technology, Danvers, MA, USA), cleaved caspase-8 (BD Pharmingen, USA), Bax and p53 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), DR4, DR5, and ß-actin (Sigma-Aldrich).

Techniques: Western Blot, Expressing, Immunocytochemistry

Journal: Oncotarget

Article Title: Enhancement of TRAIL-induced apoptosis by 5-fluorouracil requires activating Bax and p53 pathways in TRAIL-resistant lung cancers

doi: 10.18632/oncotarget.14994

Figure Lengend Snippet: A549 adenocarcinoma cells were pretreated with 5-fluorouracil at different concentrations (0, 10, 20, and 40 μM) for 12 h. A . Cells were harvested and analyzed by Western blotting to determine the expression levels of Bax; B and C . Bax expression levels determined by western blot analysis. A549 cells were pre-treated with 5-fluorouracil for 12 h and then exposed to 200 ng/mL TRAIL for an additional 1 h. β-actin was used as loading control. 5-Flu: 5-Fluorouracil; Ac-cas3: Activated caspase 3; Ac-cas8: Activated caspase 8; TRAIL: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand.

Article Snippet: Immunoblotting was performed using antibodies against cleaved caspase-3 (Cell Signaling Technology, Danvers, MA, USA), cleaved caspase-8 (BD Pharmingen, USA), Bax and p53 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), DR4, DR5, and ß-actin (Sigma-Aldrich).

Techniques: Western Blot, Expressing

Journal: Oncotarget

Article Title: Enhancement of TRAIL-induced apoptosis by 5-fluorouracil requires activating Bax and p53 pathways in TRAIL-resistant lung cancers

doi: 10.18632/oncotarget.14994

Figure Lengend Snippet: Bax-containing (Bax+/+) and Bax-deficient (Bax−/−) HCT116 human colon carcinoma cells were pretreated with 5-fluorouracil (40 μM) for 12 h. After that, cells were treated with 200 ng/mL of TRAIL protein for an additional 2 h. A . Cells were harvested and analyzed by Western blotting to determine the expression levels of Bax; B . Cell morphology photographed under light microscope (×100); C . Cell viability determined with crystal violet assay; D . Bax, Ac-cas3 and Ac-cas8 expression levels determined by western blot analysis. β-actin was used as loading control. 5-Flu: 5-Fluorouracil; Ac-cas3: Activated caspase 3; Ac-cas8: Activated caspase 8; TRAIL: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand.

Article Snippet: Immunoblotting was performed using antibodies against cleaved caspase-3 (Cell Signaling Technology, Danvers, MA, USA), cleaved caspase-8 (BD Pharmingen, USA), Bax and p53 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), DR4, DR5, and ß-actin (Sigma-Aldrich).

Techniques: Western Blot, Expressing, Light Microscopy, Crystal Violet Assay