Journal: Pflugers Archiv

Article Title: A Pore Idea: the ion conduction pathway of TMEM16/ANO proteins is composed partly of lipid

doi: 10.1007/s00424-015-1777-2

Figure Lengend Snippet: The TMEM16/Anoctamin (ANO) family tree. a A phylogenetic tree generated from 1650 TMEM16 sequences in Uniprot. Non-redundant sequences were aligned by MUSCLE and columns containing >50 % gaps were removed with TrimAl . Phylogenetic trees were constructed by CLCBIO Main Workbench 6.9 using Kimura Neighbor-Joining. The fungal TMEM16 proteins afTMEM16 and nhTMEM16 are indicated. b A subset of vertebrate TMEM16 proteins identified by Uniprot were assembled and curated to remove splice variants and duplicate sequences. The sequences were truncated by deleting (~50) variable N-terminal amino acids. Trees were displayed using Dendroscope ( http://dendroscope.org/ ). Percent identity and (similarity) refer to human proteins compared to human TMEM16A. Brief description of known disease relevance follows sequence alignments

Article Snippet: Phylogenetic trees were constructed by CLCBIO Main Workbench 6.9 using Kimura Neighbor-Joining.

Techniques: Generated, Construct, Sequencing