Journal: Current Research in Food Science

Article Title: A Pectic Polysaccharide from Lycium ruthenicum Murray Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice

doi: 10.1016/j.crfs.2024.100955

Figure Lengend Snippet: Fig. 4. Effects of LRWP-Ap on pathological changes and TJ protein expression and on the balance of Arg-1/iNOS in mouse colon tissues. (A&B) H&E staining of mouse colon tissue. H&E staining (A) and colonic tissue damage score (B). (C–F) Western blot analysis of TJ protein and β-actin protein expression in colon tissues. Grayscale intensity statistics for Claudin 1 (D), Occludin (E), and ZO-1 (F) protein expression. (G–I) Immunofluorescence images of Arg-1, iNOS, and DAPI in paraffin- embedded colon tissue sections from each group. Relative fluorescence intensity of Arg-1 (H) and iNOS (I). L–A: LRWP-Ap. The data are expressed as the means ± standard deviations (n = 3, randomly selected from 6). *P < 0.05 and **P < 0.01 vs. the control group. #P < 0.05 and ##P < 0.01 vs. the model group.

Article Snippet: The membrane was blocked with a Rapid Closure Solution (GeneFist, Shanghai, China) for 30 min, then incubated overnight at 4◦Cwith primary antibodies against ZO-1, Occludin, Claudin-1, and β-actin (Proteintech, Hubei, China).

Techniques: Expressing, Staining, Western Blot, Immunofluorescence, Fluorescence, Control

Journal: Frontiers in pharmacology

Article Title: Andrographolide Inhibits Cholangiocarcinoma Cell Migration by Down-Regulation of Claudin-1 via the p-38 Signaling Pathway.

doi: 10.3389/fphar.2019.00827

Figure Lengend Snippet: FIGURE 4 | Inhibitory effect of andrographolide on cell migration mediated by claudin-1. The RNA interference of claudin-1 was used to knock down the claudin-1 gene in KKU-M213 cells. KKU-M213 were treated with 0 μM (1% DMSO), 50 μM of andrographolide, scramble, and si-claudin-1 prior to harvesting RNA and cellular proteins for the detection of claudin-1. (A) The expression of claudin-1 mRNA from real-time RT-PCR; (B) The level of claudin-1 protein from Western blot analysis. Actin was used as internal standard. (C) With those treatments, KKU-M213 was scratched to make a wound and photographed at 0 and 12 h to measure the wound length for migration index analysis. (D) The results of bar graph are represented by the average of relative intensity compared with that of control. Data were derived from three independent experiments and are presented as mean ± SE, *p < 0.05, **p < 0.01.

Article Snippet: Small Interfering RNA Targeting of Claudin-1 Synthetic small interfering RNAs (siRNA) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Migration, Knockdown, Expressing, Quantitative RT-PCR, Western Blot, Control, Derivative Assay

Journal: Food Science and Biotechnology

Article Title: Promoting effect of the Maillard reaction products produced during the stir-frying process of Hordei Fructus Germinatus on the intestinal absorption of active ingredients in Hordei Fructus Germinatus

doi: 10.1007/s10068-021-00911-1

Figure Lengend Snippet: Effects of MRPs on the mRNA and protein expression levels of claudin-1 and ZO-1 in duodenal tissues. (A) The mRNA expression levels of claudin-1. (B) The mRNA expression levels of ZO-1. (C) The protein expression levels of claudin-1. (D) The protein expression levels of ZO-1. The mRNA levels of claudin-1and ZO-1 were quantified with β-actin as reference gene. The protein expression levels of claudin-1and ZO-1 were quantified with β-actin as the internal control. Values are mean ± SD (n = 6). *P < 0.05, compared with the control group; △P < 0.05, compared with the S-R-HGF group

Article Snippet: Rabbit polyclonal anti-ZO-1 antibody and Rabbit polyclonal anti-Claudin-1 antibody were provided by Beijing OriGene Technology Co., Ltd. (Beijing, China).

Techniques: Expressing