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Helmholtz Zentrum fur Infektionsforschung GmbH
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Biomatik
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SAS institute
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Vivogen Biotechnology Inc
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NanoCarrier Co
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A thiazoloquinolone derivative that stimulates TLR8 in human peripheral blood mononuclear cells
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Image Search Results
Journal: The Journal of Allergy and Clinical Immunology
Article Title: Toll-like receptor 8 agonist nanoparticles mimic immunomodulating effects of the live BCG vaccine and enhance neonatal innate and adaptive immune responses
doi: 10.1016/j.jaci.2016.12.985
Figure Lengend Snippet: CL075:Ag85Bp25-PS and BCG induce comparable Ag85B-specific CD4 + effector T cells in the spleens of neonatal huTLR8Tg mice. A, Experimental design: 7-day-old huTLR8Tg and matched littermate WT mice were immunized subcutaneously and killed 14 days later before harvesting spleens and staining splenocytes with a control versus Ag85B 280–294 MHCII tetramer and for surface marker expression. B, Representative flow cytometric plots showing Tet + T cells as CD44 + Neg Tet + or CD44 + Ag85B Tet + cells gated on live CD4 + splenic lymphocytes of BCG- or CL075-Ag85Bp25-PS–vaccinated mice 2 weeks after immunization. C and D, Similar to BCG, CL075-Ag85Bp25-PS induced a significant increase in CD44 + Ag85B Tet + cells compared with CD44 + Neg Tet + cells. Results represent means ± SEMs (n = 14-15), with significance relative to unspecific control tetramer. E, Neonatal humanized TLR8 mice have greater numbers of splenic Ag85B-specific CD4 + T cells versus their WT littermates. The unpaired Mann-Whitney test was applied, and statistical significance was denoted as follows: ** P < .01 and *** P < .001. NS , Not significant.
Article Snippet: Mice were immunized subcutaneously at the scruff of the neck with either 0.2 × 10 6 colony-forming units of BCG (Organon Teknika/Merck, Durham, NC) or CL075:Ag85Bp25-PS containing approximately 8 to 10 μg of
Techniques: Staining, Marker, Expressing, MANN-WHITNEY