Journal: Scientific Reports

Article Title: Design and synthesis of thiazole-based hydroxamate histone deacetylase inhibitors with potent antitumor efficacy by inducing apoptosis, pyroptosis and cell cycle arrest

doi: 10.1038/s41598-025-08474-5

Figure Lengend Snippet: 15a and 15d mediated HepG2 cell apoptosis. ( A ) Apoptosis of HepG2 cells induced by 15a , 15d , and SAHA treatment for 48 h at the indicated concentrations were examined by using Annexin V-FITC/PI double-staining analysis. ( B ) The statistical result of ( A ). ( C ) HepG2 cells were treated with 15a , 15d , and SAHA for 24 h at the indicated concentrations. The Cl-PARP and Cl-caspase 3 protein levels were examined by Western blot analysis, and β-actin was detected as the endogenous loading control, accordingly. ( D ) The statistical result of ( C ). The data are expressed as means ± SD of three separate experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 versus control.

Article Snippet: The following primary antibodies were utilized for immunoblotting, with their respective sources and dilution factors indicated: Acetylated histone H3 (Ac-H3, Beyotime, catalog #AF5620, diluted 1:1000); Acetylated histone H4 (Ac-H4, Beyotime, catalog #AF5629, diluted 1:1000); Cyclin D1 (CST, catalog #2978, diluted 1:1000); Cyclin-dependent kinase 4 (CDK4, CST, catalog #12790, diluted 1:1000); Cleaved poly (ADP-ribose) polymerase (cl-PARP, CST, catalog #5625, diluted 1:1000); Caspase 3 (CST, catalog #9662, diluted 1:1000); Cleaved caspase 3 (cl-caspase 3, CST, catalog #9661, diluted 1:1000); Anti-DFNA5/GSDME (Abcam, catalog #ab215191, diluted 1:1000); γ-H2AX (CST, catalog #9718, diluted 1:1000); β-actin (CST, catalog #3700, diluted 1:10,000); Anti-rabbit IgG Fab2 (Sigma, catalog #A0545, diluted 1:10,000); Anti-mouse IgG Fab2 (Sigma, catalog #A4416, diluted 1:10,000); Goat anti-Rabbit IgG (Invitrogen, Waltham, MA, USA, diluted 1:500).

Techniques: Double Staining, Western Blot, Control

Journal: Iranian Journal of Parasitology

Article Title: Characterization of Specific RAPD Markers of Virulence in Trichomonas vaginalis Isolates

doi:

Figure Lengend Snippet: A) RAPD banding patterns obtained using primers Tv-5, OPA-6, and OPA-11. In square the RAPD marker used for sequencing. The arrows indicate the RAPD marker position. B) Agarose gel electrophoresis 2 % of PCR for the detection of the LRR family protein gene in T. vaginalis. The clinical presentation, RAPD cluster group and TVV infection of each isolates are shown on top. The isolate names are shown on top of each gel. MM 1 : Molecular weight marker 1 kb (Promega, USA). MM 2 : Molecular weight marker Gene Ruler™ 100 bp Plus DNA ladder (Fermentas). CP: positive control (strain ATCC 30001), NC: negative control

Article Snippet: Also the sequences were compared with the complete genome of T. vaginalis (G3 strain American Type Culture Collection PARA-98, Human, 1973, New England) using the T. vaginalis genome database (TrichoDB, http://trichodb.org/trichodb) Sequence alignments were considered biologically significant when presenting E-values below 10 - 10 and 10 -2 for nucleotide and protein sequence, respectively ( ).

Techniques: Marker, Sequencing, Agarose Gel Electrophoresis, Infection, Molecular Weight, Positive Control, Negative Control

Journal: Iranian Journal of Parasitology

Article Title: Characterization of Specific RAPD Markers of Virulence in Trichomonas vaginalis Isolates

doi:

Figure Lengend Snippet: Sequences to produce a similarity using the BLASTn program for each virulence RAPD markers

Article Snippet: Also the sequences were compared with the complete genome of T. vaginalis (G3 strain American Type Culture Collection PARA-98, Human, 1973, New England) using the T. vaginalis genome database (TrichoDB, http://trichodb.org/trichodb) Sequence alignments were considered biologically significant when presenting E-values below 10 - 10 and 10 -2 for nucleotide and protein sequence, respectively ( ).

Techniques: Marker, Virus, Sequencing, Variant Assay

Journal: Frontiers in oncology

Article Title: Psorachromene Suppresses Oral Squamous Cell Carcinoma Progression by Inhibiting Long Non-coding RNA GAS5 Mediated Epithelial-Mesenchymal Transition.

doi: 10.3389/fonc.2019.01168

Figure Lengend Snippet: FIGURE 2 | Inhibition of cell cycle progression and promotion of apoptosis in OSCC cells by psorachromene. (A,B) Effect of psorachromene on cell cycle progression in SAS and OECM1 cells. The cells were treated with vehicles or different concentrations of psorachromene for 24 h. Cell cycle distribution was analyzed by flow cytometry. (C) SAS cells were treated with or without psorachromene, and cell apoptosis was determined using a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Green punctate staining (white arrows) represents TUNEL-positive cells. Apoptotic cells were identified as DAPI and TUNEL double-stained cells. Magnification: 100×. Quantitative results seen in (D). (E) Western blot analysis showing the effect on caspase 9 and PARP activity in OSCC cells after 48 h of psorachromene treatment. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Antibodies against vimentin, E-cadherin, slug, cleaved-PARP (cl-PARP, Asp214), and caspase 9 were obtained from Cell Signaling (Temecula, CA, USA).

Techniques: Inhibition, Cytometry, TUNEL Assay, Staining, Western Blot, Activity Assay