Journal: Journal of endocrinological investigation

Article Title: Quantitative ultrasound techniques and biochemical markers to assess liver steatosis and fibrosis in newly diagnosed acromegaly.

doi: 10.1007/s40618-024-02384-5

Figure Lengend Snippet: Fig. 1 The results of serum CK18, IGFBP7 and liver quantitative ultrasound

Article Snippet: Elabscience Human CK18 ELISA kit (detection range 6.25–400 mIU/mL, sensitivity < 3.75 mIU/ml, coefficient of variation (CV) < 10%) and Elabscience Human IGFBP7 ELISA (detection range 0.94–60 ng/ml, sensitivity < 0.56 ng/ml, CV < 10%) were used.

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Comparative evaluation of the therapeutic efficacy between human amniotic epithelial cells and human umbilical cord mesenchymal stem cells in premature ovarian insufficiency

doi: 10.1186/s13287-025-04881-7

Figure Lengend Snippet: Characterization of hAECs and hUC-MSCs. A Schematic illustration of the experimental design for comparing the effects of hAECs and hUC-MSCs on ovarian function. B , C Morphological feature and flow cytometric analysis of hAECs and hUC-MSCs. D , E Representative images of double immunofluorescence staining for OCT4 and CK18 or N-cadherin in hAECs and hUC-MSCs. F Multilineage differentiation potential of hUC-MSCs into osteoblasts, adipocytes, and chondrocytes, as assessed by Alizarin Red, Oil Red O, and Alcian Blue staining, respectively. Scale bars represent 25, 50, 100 and 200 μm

Article Snippet: After permeabilization with 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), cell were incubated with the following primary antibodies at 4 °C for overnight: OCT4 (1:200, Boster), CK18 (1:200, Boster) and N-cadherin (1:200, Boster).

Techniques: Double Immunofluorescence Staining, Staining

Journal: Frontiers in Immunology

Article Title: A novel oral formulation of the melanocortin-1 receptor agonist PL8177 resolves inflammation in preclinical studies of inflammatory bowel disease and is gut restricted in rats, dogs, and humans

doi: 10.3389/fimmu.2023.1083333

Figure Lengend Snippet: Representative colon immunohistochemistry for K18 sections of DSS colitis-induced rats. Sham (A) is no challenge and no treatment. Placebo/vehicle control (B) is no treatment but DSS challenge. (C) is DSS challenge and treatment with PL8177 (100 µg). (D) is DSS challenge and treatment with mesalazine. (E) Quantification of positive K18 cells in colon crypts versus total crypt cells. Notes: material was limited for sham staining resulting in 2 colon tissues. Bar chart shows mean, SEM, and data points. DSS, dextran sulfate sodium; K18, cytokeratin 18.

Article Snippet: For immunohistochemistry, colon sections were processed for antigen retrieval and immunostained with primary antibody: keratin 18 (K18) (1:400, Bioss, bs-5405R) and with a rabbit-specific biotin-conjugated secondary antibody from HistoWiz (HistoWiz, Inc. Brooklyn, NY).

Techniques: Immunohistochemistry, Control, Staining