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Millipore
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WuXi AppTec
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Cytokeratin 12 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with
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Cytokeratin 12 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with
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Buy from Supplier |
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Cytokeratin 12 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with
|
Buy from Supplier |
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Cytokeratin 12 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with
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Buy from Supplier |
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Cytokeratin 12 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with
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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: Pigment epithelium‐derived factor peptide promotes limbal stem cell proliferation through hedgehog pathway
doi: 10.1111/jcmm.14364
Figure Lengend Snippet: In vivo inhibition of Sonic Hedgehog (SHh) signalling suppressed the 44‐mer effect on limbal epithelial stem cells. (A) Partial limbal surgery. Left panel indicated schematic drawing of the destroyed limbus. Right panel indicated the representative Haematoxylin and eosin section of damaged limbus. The dotted line indicates the destroyed limbal area. (B) Immunostaining of consecutive sections for CK19 and CK12 in mouse normal eyes. (C) Representative micrograph of immunostaining of consecutive sections showed expressions of ∆Np63α and Lrig1 in damaged limbus 2 weeks after treated with the 44‐mer. Representative images are from six eyes of different mice per group. (D) An inhibitor for SHh (HPI4) in addition to 44‐mer was introduced via subconjunctival route. Histogram of immune‐positive cells in limbal area is presented as percentage of the total limbal basal cells. Representative images are from three eyes of different mice per group * P < 0.05 vs 44‐mer‐treated eyes. (E) Mice body weight before and after treatment
Article Snippet: Antibodies used in this study were △Np63α (Biolegend, San Diego, CA.), Lrig‐1 (ab36707, Abcam, Cambridge, MA), CK19 (ab15463; Abcam, Cambridge, MA) and
Techniques: In Vivo, Inhibition, Immunostaining
Journal: Journal of Cellular and Molecular Medicine
Article Title: Pigment epithelium‐derived factor peptide promotes limbal stem cell proliferation through hedgehog pathway
doi: 10.1111/jcmm.14364
Figure Lengend Snippet: The 44‐mer induces Gli1 and Gli3 expressions via ATGL signalling (A) BrdU‐positive cells were detected by immunofluorescence microscopy as described in Figure B. * P < 0.05 vs solvent or adding an inhibitor for ATGL (Atglistatin). (B) Atglistatin in addition to 44‐mer was introduced via subconjunctival route. At 2 weeks, eyeballs were harvested for immunofluorescence staining of consecutive sections for anti‐CK12, anti‐∆Np63α and anti‐Lrig1. Histogram of immune‐positive cells in limbal area is presented as percentage of the total limbal basal cells. * P < 0.05 vs 44‐mer‐treated eyes. Representative images are from three eyes of three mice per group. (C) LSC were treated with the 10 µmol/L 44‐mer for 6 h or pretreated with 10 µmol/L Atglistatin for 1 h and then incubated with the 44‐mer for an additional 6 h. Proteins were detected by Western blot analysis. * P < 0.05 vs 44‐mer‐treated cells
Article Snippet: Antibodies used in this study were △Np63α (Biolegend, San Diego, CA.), Lrig‐1 (ab36707, Abcam, Cambridge, MA), CK19 (ab15463; Abcam, Cambridge, MA) and
Techniques: Immunofluorescence, Microscopy, Staining, Incubation, Western Blot