Journal: Journal of Nanobiotechnology

Article Title: 3D printed hydrogel scaffolds combining glutathione depletion-induced ferroptosis and photothermia-augmented chemodynamic therapy for efficiently inhibiting postoperative tumor recurrence

doi: 10.1186/s12951-022-01454-1

Figure Lengend Snippet: Photothermal performance of Gel-SA-CuO hydrogel scaffolds. Temperature changing curves ( a ) and the average equilibrium temperatures ( b ) of Gel-SA-CuO scaffolds exposed to NIR laser at 0.6 W/cm 2 under dry conditions. Temperature changing curves ( c ) and the average equilibrium temperatures ( d ) of Gel-SA-CuO scaffolds exposed to NIR laser at 1.3 W/cm 2 under wet conditions. Temperature changing curves ( e ) and the average equilibrium temperatures ( f ) of Gel-SA-4CuO scaffolds under dry conditions exposed to different power density of NIR laser. Temperature changing curves ( g ) and the average equilibrium temperatures ( h ) of Gel-SA-6CuO scaffolds under wet conditions exposed to different power density of NIR laser. i The representative photothermal images of Gel-SA-6CuO scaffolds exposed to NIR laser at 1.3 W/cm 2 at indicated time points under wet conditions

Article Snippet: Then, the NIR laser (LASEVER, LSR808H-4 W-FC) was utilized to irradiate Gel-SA-CuO hydrogel scaffolds at 0.6 W/cm 2 .

Techniques:

Journal: Journal of Nanobiotechnology

Article Title: 3D printed hydrogel scaffolds combining glutathione depletion-induced ferroptosis and photothermia-augmented chemodynamic therapy for efficiently inhibiting postoperative tumor recurrence

doi: 10.1186/s12951-022-01454-1

Figure Lengend Snippet: In vitro anticancer efficacy of Gel-SA-CuO hydrogel scaffolds. Viability of H22 cells treated with indicated scaffolds with ( a ) or without ( b ) NIR irradiation. c Effect of incubation time on the viability of H22 cells treated with indicated scaffolds. d Fluorescence images of H22 cells after various treatments and stained with calcein-AM and PI. Scale bars represent 200 μm. e Detection of intracellular ROS generation of H22 cells after various treatments and stained with DCFH-DA. Scale bars represent 100 μm. f Measurement of GSH/GSSG ratio in H22 cells with indicated treatments. g GPx activity of H22 cells with various treatments. h The GPX4 expression of H22 cells with various treatments. i Detection of cell apoptosis of H22 cells under indicated treatments

Article Snippet: Then, the NIR laser (LASEVER, LSR808H-4 W-FC) was utilized to irradiate Gel-SA-CuO hydrogel scaffolds at 0.6 W/cm 2 .

Techniques: In Vitro, Irradiation, Incubation, Fluorescence, Staining, Activity Assay, Expressing

Journal: Journal of Nanobiotechnology

Article Title: 3D printed hydrogel scaffolds combining glutathione depletion-induced ferroptosis and photothermia-augmented chemodynamic therapy for efficiently inhibiting postoperative tumor recurrence

doi: 10.1186/s12951-022-01454-1

Figure Lengend Snippet: In vivo tumor therapeutic effect of Gel-SA-CuO hydrogel scaffolds. a Schematic illustration of the use of Gel-SA-CuO hydrogel scaffolds for inhibiting postsurgical recurrence. b Real-time thermal images of mice treated with Gel-SA hydrogel scaffolds and Gel-SA-6CuO hydrogel scaffolds under NIR irradiation. c Temperature curves in tumors of mice with indicated treatments over time. d Individual tumor growth curves of tumor-bearing mice after various treatments as indicated. e Relative tumor volume of mice with different treatments. f Photograph of tumors extracted from mice with different treatments on day 10. g Tumor mass of mice with indicated treatments

Article Snippet: Then, the NIR laser (LASEVER, LSR808H-4 W-FC) was utilized to irradiate Gel-SA-CuO hydrogel scaffolds at 0.6 W/cm 2 .

Techniques: In Vivo, Irradiation