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Image Search Results
Journal: bioRxiv
Article Title: Light inducible protein degradation in E. coli with LOVtag
doi: 10.1101/2023.02.25.530042
Figure Lengend Snippet: Incorporating light responsiveness into diverse proteins with the LOVtag. (a) Control of mCherry repression using a LacI-LOVtag fusion. (b) mCherry expression in response to light exposure for strains with LacI-LOVtag compared to IPTG induction (**p < 0.001, two tailed unpaired t-test). (c) Schematic of SoxS-based CRISPRa activation with a LOVtag appended to the MCP-SoxS activator. (d) CRISPRa control of mRFP1 expression in response to light (***p < 0.0001, two tailed unpaired t-test). (e) Schematic of the LOVtag appended to AcrB of the AcrAB-TolC efflux pump. IM, inner membrane; OM, outer membrane. (f) Minimum inhibitory concentration (MIC) curves of cells cultured in chloramphenicol. Wild type cells (BW25113) are compared to a Δ acrB strain complemented with AcrB-LOVtag and exposed to light or kept in the dark. (g) OD 600 of strains shown in (f) at 2.5 μg/ml chloramphenicol (***p < 0.0001, two tailed unpaired t-test). Error bars show standard deviation around the mean (n = 3 biological replicates).
Article Snippet: The
Techniques: Control, Expressing, Two Tailed Test, Activation Assay, Membrane, Concentration Assay, Cell Culture, Standard Deviation