Journal: ACS Materials Au

Article Title: 3D-Printed Biohybrid PE/PCL Biphasic Osteochondral Plug for Knee Cartilage Repair

doi: 10.1021/acsmaterialsau.5c00193

Figure Lengend Snippet: CHON-001 ATCC test on PCL–RGD and PCL–HA samples compounded in PCL (Resomer C212, Evonik Industries AG). Cell proliferation assay was performed in human chondrocytes seeded onto PCL-based materials. Fold change in chondrocyte numbers was assessed in each biomaterial compared to control (PCL) by Kruskal wallis test whereby * is p value <0.05. Representative data with 4 technical replicates per group.

Article Snippet: Human chondrocytes (CHON-001) were purchased from ATCC (Cat No: CRL-2846) and maintained in complete media (Dulbecco’s Modified Eagle’s Medium supplemented with 10% Fetal Bovine Serum and 0.1 mg/mL G418) and incubated at 37 °C with 95% Air and 5% CO 2 as per ATCC recommendation.

Techniques: Proliferation Assay, Control

Journal: ACS Materials Au

Article Title: 3D-Printed Biohybrid PE/PCL Biphasic Osteochondral Plug for Knee Cartilage Repair

doi: 10.1021/acsmaterialsau.5c00193

Figure Lengend Snippet: Porcine knee joint at 24 weeks with OC plug implanted (a), extracted sample with 68% closure measured using Fiji software (b), porcine knee joint with microfracture (c). H&E staining images for corresponding knee joints where (d) cartilage with significant viable chondrocytes are observed in sample with plug embedded (white space is the OCP) and (e) Little cartilage growth in sample with microfracture.

Article Snippet: Human chondrocytes (CHON-001) were purchased from ATCC (Cat No: CRL-2846) and maintained in complete media (Dulbecco’s Modified Eagle’s Medium supplemented with 10% Fetal Bovine Serum and 0.1 mg/mL G418) and incubated at 37 °C with 95% Air and 5% CO 2 as per ATCC recommendation.

Techniques: Software, Staining

Journal: Stem Cell Research & Therapy

Article Title: Extracellular vesicles from human adipose-derived stem cells relieve pain and inflammation in a rat model of knee osteoarthritis

doi: 10.1186/s13287-026-04932-7

Figure Lengend Snippet: Anti-inflammatory effect of hASC-EVs on IL-1β stimulated human chondrocytes (HC-OA). A Confocal images of HC-OAs treated with PKH67-labeled hASC-EVs, showing intracellular uptake. Scale bar, 20 μm. B – F The mRNA expression of TRPA1, COX-2, MMP-2, MMP-3, and MMP-9 was quantified using qPCR and normalized to GAPDH. Data are presented as means ± SD ( n = 3). ns, not significant ( p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. IL-1β only group

Article Snippet: Human OA chondrocytes (HC-OA; Cell Applications, USA) were derived from human articular cartilage obtained from donors diagnosed with osteoarthritis, as defined by the vendor.

Techniques: Labeling, Expressing

Journal: Open Veterinary Journal

Article Title: Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease

doi: 10.5455/OVJ.2024.v14.i5.12

Figure Lengend Snippet: Trilineage differentiation potency. Canine LCPD-affected femoral head-derived adherent cells (A-C) and BMMSCs (D-F) differentiated into adipocytes (A and D), osteoblasts (B and E), and chondrocytes (C and F). (A and D): Sudan III staining, (B and E): Alizarin Red staining, and (C and F): Alcian blue staining. Scale bars = 100 μm or 1 mm.

Article Snippet: To induce differentiation into chondrocytes, 5 × 10 5 cells were placed in 15 ml polypropylene tubes, centrifuged to form pellets, and cultured with canine chondrocyte differentiation medium (Cell Applications, Inc.) for 3 weeks.

Techniques: Derivative Assay, Staining

Journal: Aging Cell

Article Title: Urolithin A improves mitochondrial health, reduces cartilage degeneration, and alleviates pain in osteoarthritis

doi: 10.1111/acel.13662

Figure Lengend Snippet: (a–c) Oxygen consumption rates (OCR) showing basal (a) maximal (b) and ATP‐linked (c) mitochondrial respiration in primary healthy chondrocytes (HC), treated with DMSO or Urolithin A (UA) at 6.25 μM and 12 μM for 24 h. (technical replicates for a, 15–33; for b, 5–9; for c, 5–9). * p < 0.05; **** p < 0.0001 after one‐way ANOVA. Error bars represent mean ± SEM. (d) mRNA levels of mitophagy/autophagy genes PARK2 , SQSTM1 , MAP1LC3B , and BNIP3 in HC treated with DMSO or the indicated doses of UA for 24 h ( N = 5). * p < 0.05; *** p < 0.001 after one‐way ANOVA. Error bars represent mean ± SEM. (e) Mitotracker green fluorescent signal, representing the mitochondria quantity in cells treated with DMSO, 6.25 μM UA, 100 nM bafilomycin A1 (BafA1) or cotreated with both 6.25 μM UA and 100 nM BafA1 for 24 h. ( N = 12–18). *** p < 0.001; **** p < 0.0001 after one‐way ANOVA. Error bars represent mean ± SEM. (f, g) Representative images of HC treated as in (d) for 24 h and stained for TOM20 (green) and phospho‐ubiquitin (ph‐Ub, red). White squares indicate regions corresponding to insets. Nuclei were stained in blue with DAPI (f). Corresponding quantification of the intensity of ph‐Ub over TOM20 (g). ( N = 27–43) * p < 0.05; *** p < 0.001 after one‐way ANOVA. Error bars represent mean ± SEM. (h) Western blot of HC treated DMSO or UA at 6.25 μM for 24 h and probed with antibodies against phospho‐ubiquitin (ph‐Ub), TOM20 and HSP90 mitochondrial and cellular loading controls

Article Snippet: Human chondrocytes from healthy and OA donors were seeded in 96‐well seahorse plates (Agilent Seahorse XF96/XFe96 FluxPak, 102,416–100) and treated the day after with the indicated doses of UA in chondrocyte growth medium (Cell applications, 411–500).

Techniques: Staining, Ubiquitin Proteomics, Western Blot

Journal: Aging Cell

Article Title: Urolithin A improves mitochondrial health, reduces cartilage degeneration, and alleviates pain in osteoarthritis

doi: 10.1111/acel.13662

Figure Lengend Snippet: (a, b) Oxygen consumption rates (OCR) showing basal (a) and FCCP‐induced maximal respiration (b) in human primary chondrocytes (HC) from an OA patient, treated with DMSO or UA at 6.25 μM and 12 μM for 24 h (a). ( N = 7–10). * p < 0.05; ** p < 0.01; after one‐way ANOVA. Error bars represent mean ± SEM. (c) Mitotracker green fluorescent signal, in cells treated with DMSO, 12 μM UA, 100 nM bafilomycin A1 (BafA1) or cotreated with both 12 μM UA and 100 nM BafA1 for 24 h. ( N = 22–24). * p < 0.05, after one‐way ANOVA. Error bars represent mean ± SEM. (d, e) Representative images of HC treated as above for 24 h and stained for TOM20 (green) and phospho‐ubiquitin (ph‐Ub, red). White squares indicate the regions corresponding to insets. Nuclei were stained in blue with DAPI (D). Corresponding quantification of the intensity of ph‐Ub over TOM20 (E). ( N = 35–40) * p < 0.05; *** p < 0.001 after one‐way ANOVA. Error bars represent mean ± SEM

Article Snippet: Human chondrocytes from healthy and OA donors were seeded in 96‐well seahorse plates (Agilent Seahorse XF96/XFe96 FluxPak, 102,416–100) and treated the day after with the indicated doses of UA in chondrocyte growth medium (Cell applications, 411–500).

Techniques: Staining, Ubiquitin Proteomics