Journal: Drug and chemical toxicology

Article Title: Cyclosporine-A induced cytotoxicity within HepG2 cells by inhibiting PXR mediated CYP3A4/CYP3A5/MRP2 pathway.

doi: 10.1080/01480545.2023.2276084

Figure Lengend Snippet: Figure 4. Effect of CsA on mRNA (A–D) and protein (E–I) level of PXR, CYP3A4, CYP3A5 and MRP2 (n = 3). Bar graphs show the average signal strength. Results represent the mean ± SD of three separate measurements and are expressed relative to the control. (*p < 0.05 versus 0 μM CsA, **p < 0.01 versus 0 μM CsA; Student’s t test).

Article Snippet: Penicillin/streptomycin (#sc0002), ccK-8 kits (#c0038), total superoxide dismutase (sOD) assay kit with Wst-8 (# s0101) and lipid peroxidation malondialdehyde (MDa) assay kit (#s0131) was purchased from Beyotime (shanghai, china). aspartate transaminase (ast) detection kits (#105-000443-00) and lactate dehydrogenase (lDh) detection kits (#105-000446- 00) were purchased from shenzhen Ruimai Biological technology (shenzhen, Guangdong, china). the primary antibodies against PXR (#ab118336), cYP3a4 (#ab124921), cYP3a5 (#ab108624), and MRP2 (#172630) were purchased from abcam (cambridge, UK). the primary antibodies against β-actin (# BM0627) the hRP-conjugated goat anti-mouse (#Ba1051) and the goat anti-rabbit (#Ba1054) secondary antibodies were purchased from Boster Biological technology (Wuhan, hubei, china).

Techniques: Control

Journal: Drug and chemical toxicology

Article Title: Cyclosporine-A induced cytotoxicity within HepG2 cells by inhibiting PXR mediated CYP3A4/CYP3A5/MRP2 pathway.

doi: 10.1080/01480545.2023.2276084

Figure Lengend Snippet: Figure 6. Effect of altering PXR expression on the level of CYP3A4, CYP3A5 and MRP2. (A–C) Effect of Rif (10 μM) and KTZ (10 μM) on the mRNA level of CYP3A4, CYP3A5 and MRP2 in HepG2 cells treated with CsA (n = 5). (E, F) Effect of overexpressing and knocking-down of PXR on the mRNA level of CYP3A4, CYP3A5, and MRP2 in HepG2 cells treated with CsA (n = 5). (G) Effect of Rif (10 μM) and KTZ (10 μM) on the expression of CYP3A4, CYP3A5, and MRP2 in HepG2 cells treated with CsA (n = 5). (H) Effect of overexpressing and knocking-down of PXR on the expression of CYP3A4, CYP3A5, and MRP2 in HepG2 cells treated with CsA (n = 5) (*p < 0.05, **p < 0.01; Student’s t test).

Article Snippet: Penicillin/streptomycin (#sc0002), ccK-8 kits (#c0038), total superoxide dismutase (sOD) assay kit with Wst-8 (# s0101) and lipid peroxidation malondialdehyde (MDa) assay kit (#s0131) was purchased from Beyotime (shanghai, china). aspartate transaminase (ast) detection kits (#105-000443-00) and lactate dehydrogenase (lDh) detection kits (#105-000446- 00) were purchased from shenzhen Ruimai Biological technology (shenzhen, Guangdong, china). the primary antibodies against PXR (#ab118336), cYP3a4 (#ab124921), cYP3a5 (#ab108624), and MRP2 (#172630) were purchased from abcam (cambridge, UK). the primary antibodies against β-actin (# BM0627) the hRP-conjugated goat anti-mouse (#Ba1051) and the goat anti-rabbit (#Ba1054) secondary antibodies were purchased from Boster Biological technology (Wuhan, hubei, china).

Techniques: Expressing

Journal: Drug and chemical toxicology

Article Title: Cyclosporine-A induced cytotoxicity within HepG2 cells by inhibiting PXR mediated CYP3A4/CYP3A5/MRP2 pathway.

doi: 10.1080/01480545.2023.2276084

Figure Lengend Snippet: Figure 7. Effect of altering CYP3A4, CYP3A5, and MRP2 expression on the role of CsA. (A-B) Effect of knocking-down of CYP3A4/5 and overexpressing of CYP3A4 on AST (A) and LDH (B) level in HepG2 cells treated with CsA (n = 5). (C, D) Effect of knocking-down of MRP2 on AST (C) and LDH (D) level in HepG2 cells treated with CsA (n = 5). (**p < 0.01; Student’s t test).

Article Snippet: Penicillin/streptomycin (#sc0002), ccK-8 kits (#c0038), total superoxide dismutase (sOD) assay kit with Wst-8 (# s0101) and lipid peroxidation malondialdehyde (MDa) assay kit (#s0131) was purchased from Beyotime (shanghai, china). aspartate transaminase (ast) detection kits (#105-000443-00) and lactate dehydrogenase (lDh) detection kits (#105-000446- 00) were purchased from shenzhen Ruimai Biological technology (shenzhen, Guangdong, china). the primary antibodies against PXR (#ab118336), cYP3a4 (#ab124921), cYP3a5 (#ab108624), and MRP2 (#172630) were purchased from abcam (cambridge, UK). the primary antibodies against β-actin (# BM0627) the hRP-conjugated goat anti-mouse (#Ba1051) and the goat anti-rabbit (#Ba1054) secondary antibodies were purchased from Boster Biological technology (Wuhan, hubei, china).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Relevance of control diet choice in metabolic studies: impact in glucose homeostasis and vascular function

doi: 10.1038/s41598-020-59674-0

Figure Lengend Snippet: Composition and energetic profile of standard chow (Chow) and low-fat (LF) diet.

Article Snippet: Glucose (glucose Trinder Method, Roche Applied Science, Barcelona, Spain), triglycerides (glycerol phosphate oxidase method, Biolabo, Maizy, France), non-esterified fatty acids (Acyl-CoA oxidase method, Wako, Bioproducts, Germany) and cholesterol (cholesterol oxidase - peroxidase method, Spinreact, Barcelona, Spain) were measured by colorimetric methods.

Techniques: Fat, Maltodextrin

Journal: Scientific Reports

Article Title: Relevance of control diet choice in metabolic studies: impact in glucose homeostasis and vascular function

doi: 10.1038/s41598-020-59674-0

Figure Lengend Snippet: Effect of dietary treatment on body weight, adiposity and biochemical parameters after 12 h fasting.

Article Snippet: Glucose (glucose Trinder Method, Roche Applied Science, Barcelona, Spain), triglycerides (glycerol phosphate oxidase method, Biolabo, Maizy, France), non-esterified fatty acids (Acyl-CoA oxidase method, Wako, Bioproducts, Germany) and cholesterol (cholesterol oxidase - peroxidase method, Spinreact, Barcelona, Spain) were measured by colorimetric methods.

Techniques: Clinical Proteomics

Journal: Animal Reproduction

Article Title: Chlorogenic acid improves the quality of boar semen processed in Percoll

doi: 10.21451/1984-3143-AR2019-0021

Figure Lengend Snippet: Cholesterol concentration in seminal plasma of cooled boar semen after the addition of chlorogenic acid (CA) (4.5 mg/ml) or vitamin E (VE) (400 µg/ml) to the extender, with or without the use of the Percoll sperm selection technique. Means followed by different letters differ by Tukey’s test (P<0.05).

Article Snippet: For measurement of total cholesterol in the seminal plasma, the commercial kit Cholesterol Oxidase (Labtest Diagnosis S.A., Lagoa Santa, Brazil) was used following the manufacturer's instructions ( ).

Techniques: Concentration Assay, Clinical Proteomics, Selection