Journal: International journal of systematic and evolutionary microbiology

Article Title: Reclassification of Aurantimonas altamirensis (Jurado et al. 2006), Aurantimonas ureilytica (Weon et al. 2007) and Aurantimonas frigidaquae (Kim et al. 2008) as members of a new genus, Aureimonas gen. nov., as Aureimonas altamirensis gen. nov., comb. nov., Aureimonas ureilytica comb. nov. and Aureimonas frigidaquae comb. nov., and emended descriptions of the genera Aurantimonas and Fulvimarina.

doi: 10.1099/ijs.0.027029-0

Figure Lengend Snippet: Fig. 2. Polar lipid compositions of strains Aurantimonas coralicida DSM 14790T (a), Aurantimonas ureilytica DSM 18598T (b), Aurantimonas altamirensis DSM 21988T (c), Aurantimonas frigidaquae DSM 21987T (d) and Fulvimarina pelagi DSM 15515T (e). All data were from this study. Separation was in two dimensions: first dimension, chloroform/ methanol/water (65 : 25 : 4, v/v/v); second dimension, chloroform/methanol/acetic acid/ water (80 : 12 :15 : 4, v/v/v/v). Plates were stained with 5 % molybdophosphoric acid in ethanol and heated for 15 min at 150 6C. DPG, diphosphatidylglycerol; PG, phosphatidylgly- cerol; PE, phosphatidylethanolamine; PME, phosphatidylmonomethylethanolamine; PC, phos- phatidylcholine; AL1, AL2, unidentified aminolipids; L1–L2, lipids; SQDG, sulfoqui- novosyldiacylglycerol.

Article Snippet: Aurantimonas coralicida DSM 14790T, Aurantimonas ureilytica DSM 18598T, Aurantimonas altamirensis DSM 21988T, Aurantimonas frigidaquae DSM 21987T and Fulvimarina pelagi DSM 15513T were obtained from the DSMZ.

Techniques: Staining

Journal: Malaria Journal

Article Title: Real-time measurement of Plasmodium falciparum -infected erythrocyte cytoadhesion with a quartz crystal microbalance

doi: 10.1186/s12936-016-1374-7

Figure Lengend Snippet: Quartz coating with CD36 using membranes of melanoma cells and CSA via PLL. a Overlay picture of a C32 melanoma cell stained for DNA (DAPI, blue ) and CD36 (FITC, green ) showing a homogenous spread of CD36 on the surface and the nucleus in the centre of the cell; b fluorescence picture of isolated and homogenized cell membranes of C32 melanoma cells immobilized to a PLL-coated quartz and stained with a CD36 antibody (FITC, green ); c overlay picture of a quartz coated with PLL/CSA showing an even distribution of fluorescence signal, indicating a homogenous coating of CSA to the quartz. The quartz was stained with an anti-CSA antibody (FITC, green )

Article Snippet: C32-melanoma cells expressing large amounts of CD36 were obtained from the American Type Culture Collection (ATCC) and cultured at 5 % carbon dioxide and 37 °C using DMEM supplemented with 50 μg/ml gentamycin, 10 % FCS, 2 mM l -glutamine, and 1 % MEM non-essential amino acid solution (NEAA).

Techniques: Staining, Fluorescence, Isolation

Journal: Malaria Journal

Article Title: Real-time measurement of Plasmodium falciparum -infected erythrocyte cytoadhesion with a quartz crystal microbalance

doi: 10.1186/s12936-016-1374-7

Figure Lengend Snippet: Overview of QCM experiments measuring attachment of cells to receptors by frequency shifts and subsequent microscopic count. Median, interquartile range and single measurements of frequency shifts measured by the QCM platform F id g et Type 1 ( upper panel ) and subsequent microscopic count of the number of attached cells per microscopic field ( lower panel ) for either P. falciparum iRBCs of FCR3-CSA strain (n = 5) and red blood cells (RBC, n = 3) added to CSA receptors (CSA), or for iRBCs of FCR3-CD36 strain (n = 6) and RBCs (n = 4) added to CD36 receptors. Results show that iRBCs (FCR3 parasites) bind to the respective receptor, reflected by a dampening of the frequency and a higher count data in the microscope whereas RBCs do not bind. CSA chondroitin sulfate A, PLL poly- l -lysin

Article Snippet: C32-melanoma cells expressing large amounts of CD36 were obtained from the American Type Culture Collection (ATCC) and cultured at 5 % carbon dioxide and 37 °C using DMEM supplemented with 50 μg/ml gentamycin, 10 % FCS, 2 mM l -glutamine, and 1 % MEM non-essential amino acid solution (NEAA).

Techniques: Microscopy

Journal: Malaria Journal

Article Title: Real-time measurement of Plasmodium falciparum -infected erythrocyte cytoadhesion with a quartz crystal microbalance

doi: 10.1186/s12936-016-1374-7

Figure Lengend Snippet: Example QCM signals and pictures obtained for CD36. Measurements were carried out on the two-channel sensor platform F id g et Type 1. a The samples (iRBCs and RBCs) were injected after achievement of a stable baseline followed by a short stop-flow interval. Adhesion of iRBCs (FCR3-CD36) led to a decrease of the signal. The low frequency shift obtained with RBCs shows an unspecific drift due to the alterations in the viscosity of the fluid. Frequency shifts after 2.5 h (time frame indicated by vertical dotted lines ) are −83 Hz for RBCs and −361 Hz for iRBCs indicating the attachment of iRBCs to CD36 on the quartz; b and c pictures of corresponding quartzes stained with DAPI after measurement in the QCM platform confirming the results. After injection of iRBCs it can be clearly seen in ( b ) that iRBCs bind to the attached C32 cell membranes expressing CD36 on the quartz. In contrast in ( c ), after the experiment with RBCs only very few RBCs are detected on the sensor surface and only cell membranes can be seen

Article Snippet: C32-melanoma cells expressing large amounts of CD36 were obtained from the American Type Culture Collection (ATCC) and cultured at 5 % carbon dioxide and 37 °C using DMEM supplemented with 50 μg/ml gentamycin, 10 % FCS, 2 mM l -glutamine, and 1 % MEM non-essential amino acid solution (NEAA).

Techniques: Injection, Viscosity, Staining, Expressing