Journal: CNS Neuroscience & Therapeutics

Article Title: A nerve conduit filled with Wnt5a‐loaded fibrin hydrogels promotes peripheral nerve regeneration

doi: 10.1111/cns.13752

Figure Lengend Snippet: Characterization of the Wnt5a‐loaded fibrin hydrogel. (A–B) Gross view of the saline solution (left) and fibrin hydrogel (right) at upright and upside‐down position. (C) A representative photograph of the nerve conduit filled with the Wnt5a‐loaded fibrin hydrogel. (D) Representative SEM images. (E) The absorbance of Wnt5a when the mass ratio (w/w) of Wnt5a and fibrin hydrogel was 1.2, 1.4, 1.6, and 1.8, respectively. (F) The loading capacity (w/w%) of Wnt5a‐loaded fibrin hydrogel in each group. (G) The sustained‐release profile of Wnt5a‐loaded fibrin hydrogel

Article Snippet: We mixed 100 UI/mL thrombin (T4648, Sigma‐Aldrich) and 0.8% fibrinogen (f8630, Sigma‐Aldrich, T4648, Sigma‐Aldrich) solutions at a ratio of 1:4 to form the fibrin hydrogel., We created a Wnt5a‐loaded fibrin hydrogel by mixing the Wnt5a solution (7815‐NG, R&D Systems) in thrombin and fibrinogen solutions, respectively, before the fibrin hydrogel was synthesized.

Techniques: Saline

Journal: CNS Neuroscience & Therapeutics

Article Title: A nerve conduit filled with Wnt5a‐loaded fibrin hydrogels promotes peripheral nerve regeneration

doi: 10.1111/cns.13752

Figure Lengend Snippet: Wnt5a regulates SC proliferation and secretion. (A–C) qRT‐PCR results showed relative expression levels of VEGF, NGF, and CNTF mRNA. (D–F) ELISA results indicated the concentrations of VEGF, NGF, and CNTF. (G) CCK‐8 results showed the effect of Wnt5a on SC proliferation after incubation for 24 h and 48 h.* p < 0.05, ** p < 0.01, *** p < 0.001. Data are expressed as the mean ±SD. SCs, Schwann cells; VEGF, vascular endothelial growth factor; NGF, nerve growth factor; CNTF, cholinergic neurotrophic factor. qRT‐PCR, quantitative real‐time polymerase chain reaction

Article Snippet: We mixed 100 UI/mL thrombin (T4648, Sigma‐Aldrich) and 0.8% fibrinogen (f8630, Sigma‐Aldrich, T4648, Sigma‐Aldrich) solutions at a ratio of 1:4 to form the fibrin hydrogel., We created a Wnt5a‐loaded fibrin hydrogel by mixing the Wnt5a solution (7815‐NG, R&D Systems) in thrombin and fibrinogen solutions, respectively, before the fibrin hydrogel was synthesized.

Techniques: Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Incubation, Real-time Polymerase Chain Reaction