Journal: Autophagy

Article Title: Non-muscle MYH10/myosin IIB recruits ESCRT-III to participate in autophagosome closure to maintain neuronal homeostasis

doi: 10.1080/15548627.2023.2169309

Figure Lengend Snippet: Reagents and resources used in this study.

Article Snippet: pmRFP C1-CHMP4C , Sino Biological , HG14241-ANR.

Techniques: Protease Inhibitor, Electron Microscopy, Silver Staining, Plasmid Preparation, Negative Control, Subcloning, Recombinant, Expressing, shRNA

Journal: Frontiers in Pharmacology

Article Title: Establishment of a prognostic risk model for osteosarcoma and mechanistic investigation

doi: 10.3389/fphar.2024.1399625

Figure Lengend Snippet: Primer sequences.

Article Snippet: Subsequently, the sections were incubated with antibodies against β-catenin (A19657, ABclonal Technology, China), CHMP4C (bs-7744R, Bioss Biotechnology Co., Ltd., China), GSK3β (A2081, ABclonal Technology, China), and p-GSK3β (bs-3161R, Bioss Biotechnology Co., Ltd., China) overnight at 4°C.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Establishment of a prognostic risk model for osteosarcoma and mechanistic investigation

doi: 10.3389/fphar.2024.1399625

Figure Lengend Snippet: Risk model and survival analysis for prognostic evaluation of OS Patients. (A,B) LASSO selection analysis plots of OS-related genes within the greenyellow module. (C) The ROC curve. (D) Kaplan-Meier survival analysis results, showing gene survival analysis curves significantly associated with the prognosis of OS patients (including CHMP4C, FAM222B, PROSER2, SNORA12, ZNF200).

Article Snippet: Subsequently, the sections were incubated with antibodies against β-catenin (A19657, ABclonal Technology, China), CHMP4C (bs-7744R, Bioss Biotechnology Co., Ltd., China), GSK3β (A2081, ABclonal Technology, China), and p-GSK3β (bs-3161R, Bioss Biotechnology Co., Ltd., China) overnight at 4°C.

Techniques: Selection

Journal: Frontiers in Pharmacology

Article Title: Establishment of a prognostic risk model for osteosarcoma and mechanistic investigation

doi: 10.3389/fphar.2024.1399625

Figure Lengend Snippet: Expression of CHMP4C and β-catenin in hFOB1.19, U2OS, HOS, and MG63 cells. (A) RT-qPCR analysis of CHMP4C and β-catenin mRNA expression. (B–D) : Immunofluorescence staining to detect the expression of CHMP4C, p-GSK3β, and β-catenin proteins. The Merge image is on the left side, DAPI (blue) is above on the right side, and FITC (green) is below on the right side. * p < 0.05, *** p < 0.001, **** p < 0.0001 compared with hFOB1..19 group. ## p < 0.01, ### p < 0.001 compared with U2OS group. ^ p < 0.05, ^^^ p < 0.001 compared with HOS group.

Article Snippet: Subsequently, the sections were incubated with antibodies against β-catenin (A19657, ABclonal Technology, China), CHMP4C (bs-7744R, Bioss Biotechnology Co., Ltd., China), GSK3β (A2081, ABclonal Technology, China), and p-GSK3β (bs-3161R, Bioss Biotechnology Co., Ltd., China) overnight at 4°C.

Techniques: Expressing, Quantitative RT-PCR, Immunofluorescence, Staining

Journal: Frontiers in Pharmacology

Article Title: Establishment of a prognostic risk model for osteosarcoma and mechanistic investigation

doi: 10.3389/fphar.2024.1399625

Figure Lengend Snippet: Impacts of overexpression and knockdown of CHMP4C on the expression of GSK3β, p-GSK3β, and β-catenin in MG63 cells. (A) RT-qPCR analysis of CHMP4C, GSK3β, and β-catenin mRNA expression in MG63 cells. (B–E) : Immunofluorescence staining to assess the expression of CHMP4C, GSK3β, p-GSK3β, and β-catenin proteins in MG63 cells. The Merge image is on the left side, DAPI (blue) is above on the right side, and FITC (green) is below on the right side. ** p < 0.01, *** p < 0.001 compared with OE-NC / IN-NC group.

Article Snippet: Subsequently, the sections were incubated with antibodies against β-catenin (A19657, ABclonal Technology, China), CHMP4C (bs-7744R, Bioss Biotechnology Co., Ltd., China), GSK3β (A2081, ABclonal Technology, China), and p-GSK3β (bs-3161R, Bioss Biotechnology Co., Ltd., China) overnight at 4°C.

Techniques: Over Expression, Knockdown, Expressing, Quantitative RT-PCR, Immunofluorescence, Staining

Journal: Frontiers in Pharmacology

Article Title: Establishment of a prognostic risk model for osteosarcoma and mechanistic investigation

doi: 10.3389/fphar.2024.1399625

Figure Lengend Snippet: Assessment of the impacts of CHMP4C overexpression and knockdown on the proliferation and migration of OS Cells. (A) CCK-8 assay for MG63 cell proliferation. (B) Colony formation assay for quantifying the number and efficiency of MG63 cell colonies. (C) Transwell assay for assessing the migration of MG63 cells. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with OE-NC / IN-NC group.

Article Snippet: Subsequently, the sections were incubated with antibodies against β-catenin (A19657, ABclonal Technology, China), CHMP4C (bs-7744R, Bioss Biotechnology Co., Ltd., China), GSK3β (A2081, ABclonal Technology, China), and p-GSK3β (bs-3161R, Bioss Biotechnology Co., Ltd., China) overnight at 4°C.

Techniques: Over Expression, Knockdown, Migration, CCK-8 Assay, Colony Assay, Transwell Assay

Journal: Frontiers in Pharmacology

Article Title: Establishment of a prognostic risk model for osteosarcoma and mechanistic investigation

doi: 10.3389/fphar.2024.1399625

Figure Lengend Snippet: Impacts of CHMP4C overexpression and knockdown on the growth of nude mouse OS and the expression of downstream pathway genes. (A,B) : Effects of CHMP4C overexpression and knockdown on the weight and volume of nude mouse OS. (C) RT-qPCR analysis of mRNA expression levels of CHMP4C, GSK3β, and β-catenin in tumor tissues. (D) Immunofluorescence staining analysis of protein expression levels of CHMP4C, GSK3β, p-GSK3β, and β-catenin in tumor tissues. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared with OE-NC / IN-NC group.

Article Snippet: Subsequently, the sections were incubated with antibodies against β-catenin (A19657, ABclonal Technology, China), CHMP4C (bs-7744R, Bioss Biotechnology Co., Ltd., China), GSK3β (A2081, ABclonal Technology, China), and p-GSK3β (bs-3161R, Bioss Biotechnology Co., Ltd., China) overnight at 4°C.

Techniques: Over Expression, Knockdown, Expressing, Quantitative RT-PCR, Immunofluorescence, Staining

Journal: Frontiers in Pharmacology

Article Title: Establishment of a prognostic risk model for osteosarcoma and mechanistic investigation

doi: 10.3389/fphar.2024.1399625

Figure Lengend Snippet: Diagram of the Molecular Mechanisms underlying CHMP4C CHMP4C within OS cells. CHMP4C inhibits the expression of GSK3β in OS cells, while upregulating the expression of pGSK3β and β-catenin. The phosphorylation of GSK3β further promotes β-catenin signaling transduction, activating the pGSK3β/β-catenin signaling pathway and promoting the proliferation and migration of OS cells.

Article Snippet: Subsequently, the sections were incubated with antibodies against β-catenin (A19657, ABclonal Technology, China), CHMP4C (bs-7744R, Bioss Biotechnology Co., Ltd., China), GSK3β (A2081, ABclonal Technology, China), and p-GSK3β (bs-3161R, Bioss Biotechnology Co., Ltd., China) overnight at 4°C.

Techniques: Expressing, Transduction, Migration

Journal: Autophagy

Article Title: Non-muscle MYH10/myosin IIB recruits ESCRT-III to participate in autophagosome closure to maintain neuronal homeostasis

doi: 10.1080/15548627.2023.2169309

Figure Lengend Snippet: Reagents and resources used in this study.

Article Snippet: pEGFP C1-CHMP4C , Sino Biological , HG14241-ANG.

Techniques: Protease Inhibitor, Electron Microscopy, Silver Staining, Plasmid Preparation, Negative Control, Subcloning, Recombinant, Expressing, shRNA