Journal: Journal of Biomedical Science

Article Title: A STING signaling relay from tumor cells to macrophages mediates the improved efficacy of combination chemotherapy in pancreatic cancer

doi: 10.1186/s12929-026-01226-1

Figure Lengend Snippet: Cisplatin promotes the anti-tumor phenotype of TAMs via activation of the cGAS-STING pathway. A , B Multiplex immunofluorescence analysis of subcutaneous tumor tissues showing STING (red), TAMs (F4/80, yellow), nuclei (DAPI, blue) and co-localization (orange) (*p < 0.05); Scale bars, 400 µm; C Confocal fluorescence microscopy of BMDMs co-cultured with cisplatin-treated PDAC cells. Left: PDAC cells (GFP, green), BMDMs (F4/80, red), nuclei (DAPI, blue); Right: dsDNA (green); Scale bars, 50 µm; D qPCR analysis of phagocytosis-related gene expression ( Sirpa , Mertk , Mrc1 ) in BMDMs co-cultured with PDAC cells (*p < 0.05, ***p < 0.001); E , F Western blot analysis of cGAS-STING pathway-related proteins and iNOS in BMDMs; G Flow cytometry analysis of CD206 (M2 marker) and CD80 (M1 marker) expression in BMDMs after cisplatin treatment (*p < 0.05, **p < 0.01, ***p < 0.001); H , I RNA sequencing analysis of a panel of M1-related genes in BMDMs

Article Snippet: The antibodies used were as follows: β-actin (sc-8432, Santa Cruz), cGAS (31659, CST), STING (13647, CST), p-STING (50907, CST), IRF3 (4302, CST), p-IRF3 (29047, CST), TBK1 (3504, CST), p-TBK1 (5483, CST), PD-L1 (13684, CST), and iNOS (13120, CST).

Techniques: Activation Assay, Multiplex Assay, Immunofluorescence, Fluorescence, Microscopy, Cell Culture, Gene Expression, Western Blot, Flow Cytometry, Marker, Expressing, RNA Sequencing

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Airway Epithelial cGAS Is Critical for Induction of Experimental Allergic Airway Inflammation.

doi: 10.4049/jimmunol.1900869

Figure Lengend Snippet: FIGURE 7. Cytosolic dsDNA accumulation was partially inhibited by Mito-TEMPO and colocalized with cGAS in IL-33–treated HBE cells. (A) Representative images of immunofluorescence staining for dsDNA in HBE cells treated with IL-33 and Mito-TEMPO in vitro. The quantitative results of cytosolic dsDNA abundance of three independent experiments in HBE cells were shown (10–20 cells per experiment). (B) Relative mRNA levels of GM-CSF in HBE cells treated with IL-33 and Mito-TEMPO in vitro. Data are the mean 6 SEM of three independent experiments. (C) Representative images of immunofluorescence staining for dsDNA (staining red) and cGAS (staining green) in HBE cells treated with IL-33 in vitro. The quantitative results of cytosolic dsDNA colocalized with cGAS in HBE cells were shown. Data are mean 6 SEM of four independent experiments, with 10–20 cells per experiment. Scale bars, 10 mm. *p , 0.05, ***p , 0.001, ****p , 0.0001.

Article Snippet: Cells are stimulated by IL-33/OVA after transfection for 24 h. Immunofluorescence analysis of dsDNA and cGAS levels in HBE cells and lung tissues of asthmatic mice Sections of lung tissues of mice were immunostained with dsDNA Ab (ab27156; Abcam), CC10 Ab (ab40873; Abcam), or cGAS Ab (26416-1- AP; Proteintech) according to the manufacturer’s instructions.

Techniques: Staining, In Vitro

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Activation of Stimulator of Interferon Genes in Hepatocytes Suppresses the Replication of Hepatitis B Virus

doi: 10.1128/aac.00771-17

Figure Lengend Snippet: Figure 1. Stable expression of cGAS and STING in HepAD38 cells successfully 574

Article Snippet: Human cGAS cDNA clone was 122 purchased from OriGene and the coding region was subcloned into puromycin resistant gene 123 expressing retroviral vector pQCXIP (Clontech) between Not I and BamH I sites.

Techniques: Expressing