Journal: PLoS ONE

Article Title: Expression of Wild-Type CFTR Suppresses NF-κB-Driven Inflammatory Signalling

doi: 10.1371/journal.pone.0011598

Figure Lengend Snippet: A . NF-κB activity in H441 cells transfected with either empty vector or wt-CFTR (400 ng), each treated with 10 µM CFTR inh172 for 4 hours, n = 3 *p<0.05 (compared to empty vector control), # p<0.05 (compared to CFTR control). B . NF-κB activity in 16HBE14o − cells treated with 10 µM CFTR inh172 and/or 10 µM forskolin and 100 µM IBMX for 4 h, n = 7, *p<0.05. C . IL-8 levels in supernatants from 16HBE14o − cells measured by ELISA, n = 8, p not significant. D IL-8 levels in supernatants from primary human nasal epithelial cells treated with 10 µM CFTR inh172 for 4 hours, n = 4, *p<0.05.

Article Snippet: 24 h post transfection, cells were treated with combinations of 10 ng/ml TNFα, to stimulate NF-κB activity, 10 µM CFTR inh172 (Merck, Nottingham, U.K.), to inhibit CFTR and 10 µM Forskolin and 10 µM IBMX to stimulate PKA by elevating cAMP.

Techniques: Activity Assay, Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay

Journal: FEBS Open Bio

Article Title: PF ‐06409577 inhibits renal cyst progression by concurrently inhibiting the mTOR pathway and CFTR channel activity

doi: 10.1002/2211-5463.13459

Figure Lengend Snippet: PF‐06409577 inhibited CFTR‐dependent short‐circuit current ( I SC ) in MDCK cells. (A) Representative I SC trace in MDCK cells. (B) Quantification of the normalized CFTR‐dependent I SC in MDCK cells pre‐treated with vehicle or different concertation of PF‐06409577 (10 or 30 n m ). Data are presented as means ± SEM. n = 3, * P < 0.05, ** P < 0.01 vs. Control (CON), one‐way ANOVA followed by Tukey's analysis.

Article Snippet: CFTR Inh ‐172 (#SF9110) was from Beyotime Biotechnology (Shanghai, China).

Techniques: Control