cfse Search Results


carboxyfluorescein diacetate succinimidyl ester cfse  (Dojindo Labs)
95
Dojindo Labs carboxyfluorescein diacetate succinimidyl ester cfse
FIGURE 5. Effects of Runx1 overexpression on IL-2 expression and cell proliferation. A, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/anti-CD28 for 24 h. The cells were stained for intracellular IL-2 and analyzed via flow cytometry. Numbers indicate the mean fluorescence intensity (MFI) of IL-2 and the percentage of IL-2-hi fractions, respectively. B, CD4 T cells derived from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice were labeled with <t>CFSE</t> and stimulated with anti- CD3/anti-CD28 antibodies. On days 0, 2, and 4, CFSE fluorescence intensities were examined via flow cytometry. Percentages of CFSE-lo/med fractions from individual mice are shown. C, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/an- ti-CD28 in the presence or absence of IL-2. The cell number was counted at 24 and 48 h and plotted. Shown are mean S.D. of three independent cultures. In each of A, B, and C, representative data from three independent but repro- ducible experiments are shown.
Carboxyfluorescein Diacetate Succinimidyl Ester Cfse, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cfda se  (MedChemExpress)
96
MedChemExpress cfda se
FIGURE 5. Effects of Runx1 overexpression on IL-2 expression and cell proliferation. A, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/anti-CD28 for 24 h. The cells were stained for intracellular IL-2 and analyzed via flow cytometry. Numbers indicate the mean fluorescence intensity (MFI) of IL-2 and the percentage of IL-2-hi fractions, respectively. B, CD4 T cells derived from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice were labeled with <t>CFSE</t> and stimulated with anti- CD3/anti-CD28 antibodies. On days 0, 2, and 4, CFSE fluorescence intensities were examined via flow cytometry. Percentages of CFSE-lo/med fractions from individual mice are shown. C, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/an- ti-CD28 in the presence or absence of IL-2. The cell number was counted at 24 and 48 h and plotted. Shown are mean S.D. of three independent cultures. In each of A, B, and C, representative data from three independent but repro- ducible experiments are shown.
Cfda Se, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cfse  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology cfse
FIGURE 5. Effects of Runx1 overexpression on IL-2 expression and cell proliferation. A, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/anti-CD28 for 24 h. The cells were stained for intracellular IL-2 and analyzed via flow cytometry. Numbers indicate the mean fluorescence intensity (MFI) of IL-2 and the percentage of IL-2-hi fractions, respectively. B, CD4 T cells derived from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice were labeled with <t>CFSE</t> and stimulated with anti- CD3/anti-CD28 antibodies. On days 0, 2, and 4, CFSE fluorescence intensities were examined via flow cytometry. Percentages of CFSE-lo/med fractions from individual mice are shown. C, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/an- ti-CD28 in the presence or absence of IL-2. The cell number was counted at 24 and 48 h and plotted. Shown are mean S.D. of three independent cultures. In each of A, B, and C, representative data from three independent but repro- ducible experiments are shown.
Cfse, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cfse  (Cytek Biosciences)
94
Cytek Biosciences cfse
FIGURE 5. Effects of Runx1 overexpression on IL-2 expression and cell proliferation. A, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/anti-CD28 for 24 h. The cells were stained for intracellular IL-2 and analyzed via flow cytometry. Numbers indicate the mean fluorescence intensity (MFI) of IL-2 and the percentage of IL-2-hi fractions, respectively. B, CD4 T cells derived from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice were labeled with <t>CFSE</t> and stimulated with anti- CD3/anti-CD28 antibodies. On days 0, 2, and 4, CFSE fluorescence intensities were examined via flow cytometry. Percentages of CFSE-lo/med fractions from individual mice are shown. C, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/an- ti-CD28 in the presence or absence of IL-2. The cell number was counted at 24 and 48 h and plotted. Shown are mean S.D. of three independent cultures. In each of A, B, and C, representative data from three independent but repro- ducible experiments are shown.
Cfse, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cfda se fluorescent probe  (Beyotime)
96
Beyotime cfda se fluorescent probe
FIGURE 5. Effects of Runx1 overexpression on IL-2 expression and cell proliferation. A, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/anti-CD28 for 24 h. The cells were stained for intracellular IL-2 and analyzed via flow cytometry. Numbers indicate the mean fluorescence intensity (MFI) of IL-2 and the percentage of IL-2-hi fractions, respectively. B, CD4 T cells derived from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice were labeled with <t>CFSE</t> and stimulated with anti- CD3/anti-CD28 antibodies. On days 0, 2, and 4, CFSE fluorescence intensities were examined via flow cytometry. Percentages of CFSE-lo/med fractions from individual mice are shown. C, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/an- ti-CD28 in the presence or absence of IL-2. The cell number was counted at 24 and 48 h and plotted. Shown are mean S.D. of three independent cultures. In each of A, B, and C, representative data from three independent but repro- ducible experiments are shown.
Cfda Se Fluorescent Probe, supplied by Beyotime, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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carboxyfluorescein diacetate succinimidyl ester  (Dojindo Labs)
93
Dojindo Labs carboxyfluorescein diacetate succinimidyl ester
FIGURE 5. Effects of Runx1 overexpression on IL-2 expression and cell proliferation. A, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/anti-CD28 for 24 h. The cells were stained for intracellular IL-2 and analyzed via flow cytometry. Numbers indicate the mean fluorescence intensity (MFI) of IL-2 and the percentage of IL-2-hi fractions, respectively. B, CD4 T cells derived from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice were labeled with <t>CFSE</t> and stimulated with anti- CD3/anti-CD28 antibodies. On days 0, 2, and 4, CFSE fluorescence intensities were examined via flow cytometry. Percentages of CFSE-lo/med fractions from individual mice are shown. C, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/an- ti-CD28 in the presence or absence of IL-2. The cell number was counted at 24 and 48 h and plotted. Shown are mean S.D. of three independent cultures. In each of A, B, and C, representative data from three independent but repro- ducible experiments are shown.
Carboxyfluorescein Diacetate Succinimidyl Ester, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cfse dye  (Elabscience Biotechnology)
94
Elabscience Biotechnology cfse dye
FIGURE 5. Effects of Runx1 overexpression on IL-2 expression and cell proliferation. A, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/anti-CD28 for 24 h. The cells were stained for intracellular IL-2 and analyzed via flow cytometry. Numbers indicate the mean fluorescence intensity (MFI) of IL-2 and the percentage of IL-2-hi fractions, respectively. B, CD4 T cells derived from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice were labeled with <t>CFSE</t> and stimulated with anti- CD3/anti-CD28 antibodies. On days 0, 2, and 4, CFSE fluorescence intensities were examined via flow cytometry. Percentages of CFSE-lo/med fractions from individual mice are shown. C, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/an- ti-CD28 in the presence or absence of IL-2. The cell number was counted at 24 and 48 h and plotted. Shown are mean S.D. of three independent cultures. In each of A, B, and C, representative data from three independent but repro- ducible experiments are shown.
Cfse Dye, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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succinimidyl ester cfda se cell proliferation assay  (Beyotime)
93
Beyotime succinimidyl ester cfda se cell proliferation assay
Biosafety and chemical stability. (A) The cytotoxicity characterized by the cell division rate that was determined using the <t>CFDA</t> SE staining of HEK293-hACE2 cells treated with Omi-X. (B) The cell cycle analysis of HEK293-hACE2 cells treated with different concentrations of Omi-X. The percentage of cells in different phases was presented as bar graphs. (C) HPLC spectrum of chemical stability of Omi-X.
Succinimidyl Ester Cfda Se Cell Proliferation Assay, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cfse  (Selleck Chemicals)
93
Selleck Chemicals cfse
Biosafety and chemical stability. (A) The cytotoxicity characterized by the cell division rate that was determined using the <t>CFDA</t> SE staining of HEK293-hACE2 cells treated with Omi-X. (B) The cell cycle analysis of HEK293-hACE2 cells treated with different concentrations of Omi-X. The percentage of cells in different phases was presented as bar graphs. (C) HPLC spectrum of chemical stability of Omi-X.
Cfse, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cfse controls  (Bio-Rad)
93
Bio-Rad cfse controls
Biosafety and chemical stability. (A) The cytotoxicity characterized by the cell division rate that was determined using the <t>CFDA</t> SE staining of HEK293-hACE2 cells treated with Omi-X. (B) The cell cycle analysis of HEK293-hACE2 cells treated with different concentrations of Omi-X. The percentage of cells in different phases was presented as bar graphs. (C) HPLC spectrum of chemical stability of Omi-X.
Cfse Controls, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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7 aad reagent  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc 7 aad reagent
Biosafety and chemical stability. (A) The cytotoxicity characterized by the cell division rate that was determined using the <t>CFDA</t> SE staining of HEK293-hACE2 cells treated with Omi-X. (B) The cell cycle analysis of HEK293-hACE2 cells treated with different concentrations of Omi-X. The percentage of cells in different phases was presented as bar graphs. (C) HPLC spectrum of chemical stability of Omi-X.
7 Aad Reagent, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cfse  (TargetMol)
93
TargetMol cfse
Biosafety and chemical stability. (A) The cytotoxicity characterized by the cell division rate that was determined using the <t>CFDA</t> SE staining of HEK293-hACE2 cells treated with Omi-X. (B) The cell cycle analysis of HEK293-hACE2 cells treated with different concentrations of Omi-X. The percentage of cells in different phases was presented as bar graphs. (C) HPLC spectrum of chemical stability of Omi-X.
Cfse, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 5. Effects of Runx1 overexpression on IL-2 expression and cell proliferation. A, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/anti-CD28 for 24 h. The cells were stained for intracellular IL-2 and analyzed via flow cytometry. Numbers indicate the mean fluorescence intensity (MFI) of IL-2 and the percentage of IL-2-hi fractions, respectively. B, CD4 T cells derived from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice were labeled with CFSE and stimulated with anti- CD3/anti-CD28 antibodies. On days 0, 2, and 4, CFSE fluorescence intensities were examined via flow cytometry. Percentages of CFSE-lo/med fractions from individual mice are shown. C, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/an- ti-CD28 in the presence or absence of IL-2. The cell number was counted at 24 and 48 h and plotted. Shown are mean S.D. of three independent cultures. In each of A, B, and C, representative data from three independent but repro- ducible experiments are shown.

Journal: Journal of Biological Chemistry

Article Title: Down-regulation of Runx1 Expression by TCR Signal Involves an Autoregulatory Mechanism and Contributes to IL-2 Production

doi: 10.1074/jbc.m110.166694

Figure Lengend Snippet: FIGURE 5. Effects of Runx1 overexpression on IL-2 expression and cell proliferation. A, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/anti-CD28 for 24 h. The cells were stained for intracellular IL-2 and analyzed via flow cytometry. Numbers indicate the mean fluorescence intensity (MFI) of IL-2 and the percentage of IL-2-hi fractions, respectively. B, CD4 T cells derived from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice were labeled with CFSE and stimulated with anti- CD3/anti-CD28 antibodies. On days 0, 2, and 4, CFSE fluorescence intensities were examined via flow cytometry. Percentages of CFSE-lo/med fractions from individual mice are shown. C, CD4 T cells were prepared from dRunx1-tg and dRunx1-tg; CD4-Cre-tg mice and stimulated with anti-CD3/an- ti-CD28 in the presence or absence of IL-2. The cell number was counted at 24 and 48 h and plotted. Shown are mean S.D. of three independent cultures. In each of A, B, and C, representative data from three independent but repro- ducible experiments are shown.

Article Snippet: In some cases, the isolated CD4 T cells were first labeled with 10 M carboxyfluorescein diacetate succinimidyl ester (CFSE) (Dojindo, Kumamoto, Japan) in PBS containing 0.1% (w/v) BSA at 37 °C for 10 min before stimulation.

Techniques: Over Expression, Expressing, Staining, Flow Cytometry, Fluorescence, Derivative Assay, Labeling

Biosafety and chemical stability. (A) The cytotoxicity characterized by the cell division rate that was determined using the CFDA SE staining of HEK293-hACE2 cells treated with Omi-X. (B) The cell cycle analysis of HEK293-hACE2 cells treated with different concentrations of Omi-X. The percentage of cells in different phases was presented as bar graphs. (C) HPLC spectrum of chemical stability of Omi-X.

Journal: Chemical & Biomedical Imaging

Article Title: Natural Selection-Guided ACE2-Targeted Molecular Imaging: A New Paradigm for PET Tracer Development

doi: 10.1021/cbmi.5c00053

Figure Lengend Snippet: Biosafety and chemical stability. (A) The cytotoxicity characterized by the cell division rate that was determined using the CFDA SE staining of HEK293-hACE2 cells treated with Omi-X. (B) The cell cycle analysis of HEK293-hACE2 cells treated with different concentrations of Omi-X. The percentage of cells in different phases was presented as bar graphs. (C) HPLC spectrum of chemical stability of Omi-X.

Article Snippet: The cell-division rate was assessed using Carboxyfluorescein diacetate, succinimidyl ester (CFDA SE) Cell Proliferation Assay and Tracking Kit (Beyotime Biotechnology) according to the manufacturer’s protocol.

Techniques: Staining, Cell Cycle Assay