Journal: Frontiers in Immunology

Article Title: Functional variant rs12614 in CFB confers a low risk of IgA nephropathy by attenuating complement alternative pathway activation in Han Chinese

doi: 10.3389/fimmu.2022.973169

Figure Lengend Snippet: Associations of CFB gene polymorphisms with the risk of IgAN.

Article Snippet: A eukaryotic expression plasmid containing the coding sequence of CFB , either the rs12614-C allele (CS-Q0537-M35) or the rs12614-T allele (EX-Q0537-M35), was constructed (GeneCopoeia, MD, USA).

Techniques:

Journal: Frontiers in Immunology

Article Title: Functional variant rs12614 in CFB confers a low risk of IgA nephropathy by attenuating complement alternative pathway activation in Han Chinese

doi: 10.3389/fimmu.2022.973169

Figure Lengend Snippet: Associations of SNP rs12614 with clinicopathological parameters in IgAN cases.

Article Snippet: A eukaryotic expression plasmid containing the coding sequence of CFB , either the rs12614-C allele (CS-Q0537-M35) or the rs12614-T allele (EX-Q0537-M35), was constructed (GeneCopoeia, MD, USA).

Techniques:

Journal: Frontiers in Immunology

Article Title: Functional variant rs12614 in CFB confers a low risk of IgA nephropathy by attenuating complement alternative pathway activation in Han Chinese

doi: 10.3389/fimmu.2022.973169

Figure Lengend Snippet: Comparison of serum levels of CFB, CFH, and C3a among different genotypes of rs12614. The serum levels of CFB (A, B) , CFH (C, D) , and C3a (E, F) in patients with IgAN and healthy controls among different genotypes of rs12614 (CC or CT+TT). The bars represent the mean (standard deviation) or median (interquartile range). The P -value was determined by Student’s t -test or the non-parametric Mann-Whitney U -test.

Article Snippet: A eukaryotic expression plasmid containing the coding sequence of CFB , either the rs12614-C allele (CS-Q0537-M35) or the rs12614-T allele (EX-Q0537-M35), was constructed (GeneCopoeia, MD, USA).

Techniques: Comparison, Standard Deviation, MANN-WHITNEY

Journal: Frontiers in Immunology

Article Title: Functional variant rs12614 in CFB confers a low risk of IgA nephropathy by attenuating complement alternative pathway activation in Han Chinese

doi: 10.3389/fimmu.2022.973169

Figure Lengend Snippet: Effect of rs12614 (R32W mutation) on the stability of the CFB protein. (A) System flexibility analysis of CFB 32R and CFB 32W . A molecular docking search was performed followed by all-atom, explicit water MD simulations. The root mean square deviation (RMSD) of the backbone of CFB 32W (3.5107 Å) was larger than that of CFB 32R (2.7145 Å). (B, C) Final stable structures of CFB 32R (B) and CFB 32W (C) . A clustering strategy was applied to the MD trajectory. The cluster center after the equilibrium of the system was selected as the final stable complex of luteolin with CFB 32R and CFB 32W . The 10-residue-length loops containing the R32 and W32 are colored in purple.

Article Snippet: A eukaryotic expression plasmid containing the coding sequence of CFB , either the rs12614-C allele (CS-Q0537-M35) or the rs12614-T allele (EX-Q0537-M35), was constructed (GeneCopoeia, MD, USA).

Techniques: Mutagenesis, Residue

Journal: Frontiers in Immunology

Article Title: Functional variant rs12614 in CFB confers a low risk of IgA nephropathy by attenuating complement alternative pathway activation in Han Chinese

doi: 10.3389/fimmu.2022.973169

Figure Lengend Snippet: Effect of rs12614 on the expression of CFB protein. Plasmids carrying rs12614-C (CFB 32R ) or rs12614-T (CFB 32W ) were transfected into HEK293 cells. The expression levels of CFB in the cell supernatants were measured by ELISA. The data were replicated in three independent experiments. The bars represent median (interquartile range). The P -value was tested by the non-parametric Mann-Whitney U -test.

Article Snippet: A eukaryotic expression plasmid containing the coding sequence of CFB , either the rs12614-C allele (CS-Q0537-M35) or the rs12614-T allele (EX-Q0537-M35), was constructed (GeneCopoeia, MD, USA).

Techniques: Expressing, Transfection, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY