Journal: BMC Infectious Diseases

Article Title: Innate immunity glycoprotein gp-340 variants may modulate human susceptibility to dental caries

doi: 10.1186/1471-2334-7-57

Figure Lengend Snippet: Association of gp-340 I with increased S. mutans adhesion . (A-C) Bacterial adhesion to purified gp-340 I toIII proteins coated on hydroxyapatite surfaces (2 ug/ml for fixed amounts of protein). Data are presented as mean ± SE from double measurements of repeated experimental runs. (A) Adhesion of S. mutans Ingbritt to gp-340 I vs II or III (* p < 0.001, respectively). Similar results were obtained irrespective of culturing in BHI or Jordan broth. (B) Adhesion of S. mutans NG8 and isogenic mutant 834, disrupted in the pac (AgI/II polypeptide) gene, to gp-340 I protein (* p < 0.001). (C) Adhesion of L. lactis expressing SpaP (from S. mutans ) or vector control (wild-type) L. lactis MG1363 to serial dilutions of gp-340 I vs II or III (* p < 0.001, respectively). Similar results were obtained when L. lactis expressing PAc was bound to serial dilutions of gp-340 I to III.

Article Snippet: Briefly, after hydration of the hydroxyapatite beads (5 mg/well, Macro-Prep ceramic hydroxyapatite Type II, 80 μm, Bio-Rad) in buffered KCl (1 mM KH 2 PO 4 -K 2 HPO 4 buffer, pH 6.5, containing 50 mM KCl, 1 mM CaCl 2 and 0.1 mM MgCl 2 ) overnight at 4°C, the beads were coated with gp-340 protein (1–6 or 2 μg/ml in buffered KCl) for 60 minutes at room temperature.

Techniques: Purification, Mutagenesis, Expressing, Plasmid Preparation, Control