cept Search Results


93
MedChemExpress cept cocktail
(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
Cept Cocktail, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Tocris 1x cept chemical cocktail
(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
1x Cept Chemical Cocktail, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Micronor Inc ortho-cept
(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
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(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
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Cerus Inc inter-cept blood system
(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
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90
Ortho McNeil Pharmaceutical ortho-cept
(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
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90
Maglev Inc coaxial cept structure
(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
Coaxial Cept Structure, supplied by Maglev Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Huangshi Feiyun Pharmaceutical Co Ltd chemically enhanced primary treatment (cept)
(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
Chemically Enhanced Primary Treatment (Cept), supplied by Huangshi Feiyun Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Veolia Water cept-mbbr
(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
Cept Mbbr, supplied by Veolia Water, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Osel Inc muco-cept
(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
Muco Cept, supplied by Osel Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
STEMCELL Technologies Inc e8 medium + 10% dmso + cept
(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
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FUJIFILM culturesuretm cept cocktail
(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and <t>image-based</t> <t>passaging</t> at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The <t>CEPT</t> cocktail was used at every passage for 24 h to optimize cell viability.
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Image Search Results


(A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and image-based passaging at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The CEPT cocktail was used at every passage for 24 h to optimize cell viability.

Journal: bioRxiv

Article Title: Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells

doi: 10.1101/2020.08.03.235242

Figure Lengend Snippet: (A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and image-based passaging at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The CEPT cocktail was used at every passage for 24 h to optimize cell viability.

Article Snippet: To improve cell survival and provide cytoprotection during cell passaging of pluripotent and differentiated cells, we used the recently developed CEPT cocktail consisting of 50 nM Chroman 1 (#HY-15392; MedChem Express), 5 μM Emricasan (#S7775; Selleckchem), Polyamine supplement (#P8483, 1:1000 dilution; Sigma-Aldrich), and 0.7 μM Trans-ISRIB (#5284; Tocris).

Techniques: Cell Culture, Immunocytochemistry, Comparison, Passaging, Produced