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Cerus Inc
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Ortho McNeil Pharmaceutical
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Huangshi Feiyun Pharmaceutical Co Ltd
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Osel Inc
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STEMCELL Technologies Inc
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Image Search Results
Journal: bioRxiv
Article Title: Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells
doi: 10.1101/2020.08.03.235242
Figure Lengend Snippet: (A) Automated cell culture used has several characteristics and advantages as summarized in the boxed area. (B) Representative overview of pluripotent cells growing in densely packed colonies (magnification, 5x). (C) Colony with hiPSCs showing the typical morphological features of human pluripotent cells (magnification, 20x). (D) Immunocytochemistry showing that the vast majority of cells express pluripotency-associated markers OCT4 and NANOG (magnification, 5x). (E) Long-term culture of hiPSCs and maintenance of normal karyotypes. (F) Agilent Seahorse XF Glycolysis Stress Test profile shows the extracellular acidification rate (ECAR) representing key parameters of glycolytic function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (glucose, oligomycin, 2-deoxyglucose [2-DG]) were performed at indicated time points. (G) The Agilent Seahorse XF Mitochondrial Stress Test profile shows the oxygen consumption rate (OCR) representing key parameters of mitochondrial function in hESCs and hiPSCs maintained by CSTS. Serial injections of metabolic modulators (oligomycin, FCCP and Rotenone/Antimycin A (Ret/AA)) were performed at indicated time points. (H) Comparison of media change intervals during automated and manual cell culture. (I-O) The supernatant of cultures maintained either manually or robotically was analyzed by using the Vi-Cell MetaFLEX Bioanalyte Analyzer (Beckman). Box plots show the variation of fresh and spent media. See also . (P) Confluency measurement allows precise monitoring of cell growth and image-based passaging at defined timepoints. (Q) Automated cell expansion strategy showing that massive cell numbers can be produced in only 12 days. The CEPT cocktail was used at every passage for 24 h to optimize cell viability.
Article Snippet: To improve cell survival and provide cytoprotection during cell passaging of pluripotent and differentiated cells, we used the recently developed
Techniques: Cell Culture, Immunocytochemistry, Comparison, Passaging, Produced