Journal: Cell Reports

Article Title: Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals

doi: 10.1016/j.celrep.2022.110610

Figure Lengend Snippet:

Article Snippet: pLVX-GFP-Centrin-1 , Gift from Manuel Thery , Addgene #73331.

Techniques: Recombinant, Software

Journal: Biology Open

Article Title: The snRNA-processing complex, Integrator, is required for ciliogenesis and dynein recruitment to the nuclear envelope via distinct mechanisms

doi: 10.1242/bio.20136981

Figure Lengend Snippet: RPE cells were transfected with siRNA, serum-starved, fixed, and stained for acetylated tubulin, γ-tubulin, and DNA. (A–J) Representative images show decreased PC formation after knockdown of most INT subunits tested. Scale bars, 10 (A,B) or 5 (C–J) µm. (K) Quantification of PC formation (normalized to NT-siRNA) in INT-depleted cells. Gray, P <0.0001; black, not significant (both relative to CETN2-siRNA, red). (L) Comparison of INT subunit requirements in snRNA processing , dynein localization, and ciliogenesis (presented herein). (+), required; (−), not required; (N.D.), not determined.

Article Snippet: Primary antibodies were used as follows: acetylated tubulin (6-11B-1, 1:500, Sigma-Aldrich), γ-Tubulin (ab16504, 1:500, Abcam, Cambridge, MA), CEP164 (NBP-77006, 1:100, Novus Biologicals, Littleton, CO), CEP89 (HPA040056, 1:100, Sigma), FBF-1 (HPA023677; 1:100, Sigma), CETN2 (N-17-R, 1:200, Santa Cruz Biotechnology, Dallas, TX), PCTN (ab4448, 1:2000, Abcam), Ninein (ab4447, 1:500, Abcam), dynein intermediate chain (74.1, 1:500, Abcam), and PH3 (Mitosis Marker, 1:1000, Millipore, Billerica, MA).

Techniques: Transfection, Staining, Knockdown, Comparison