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Image Search Results
Journal: International Journal of Oncology
Article Title: E7 oncoprotein from human papillomavirus 16 alters claudins expression and the sealing of epithelial tight junctions
doi: 10.3892/ijo.2020.5105
Figure Lengend Snippet: Stable expression of E7 in MDCK monolayers induces the development of a higher peak of TER and changes the expression of claudins. (A) Parental MDCK and MDCK-E7 monolayers plated in Transwell inserts were subjected to a Ca-switch and the development of TER was measured using the automated cell monitoring system, cellZscope. Data are presented the mean ± SD from three independent monolayers per condition plated on Transwell inserts. Statistical analysis was performed at the 18 h with Student's t-test. **** P<0.0001. (B) Immunofluorescence detection of Cl-1, -2, -4 and -10 at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Representative images from three independent experiments. Scale bar, 10 µ m. (C) Mean fluorescence intensity measurements of Cl-1, -2, -4 and -10 performed at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Data derived from three independent images from each condition and from three independent experiments. Statistical analysis was performed using Student's t-test. Data are presented as the mean ± standard deviation. **** P<0.0001. (D) Western blot analysis of Cl-1, -2, -4 and -10 at the peak of TER (18 h) in parental and MDCK-E7 monolayers. Representative western blots of at least three independent experiments (left panel) and the densitometry analysis (right panel). Statistical analysis was performed using Student's t-test. Data are presented as the mean ± standard deviation. * P<0.05, ** P<0.01, **** P<0.0001. Ca, calcium; Cl, claudin; TER, transepithelial electrical resistance; MDCK, Madin-Darby Canine Kidney; MDCK-E7, MDCK cells transfected with E7 from HPV; HPV, human papillomavirus.
Article Snippet: TER values were obtained using the
Techniques: Expressing, Immunofluorescence, Fluorescence, Derivative Assay, Standard Deviation, Western Blot, Transfection