Journal: Neurotherapeutics

Article Title: Neuron-glioma synaptic transmission amplified by free 19S proteasome-mediated AMPAR deubiquitination promotes tumor progression

doi: 10.1016/j.neurot.2026.e00847

Figure Lengend Snippet: Free 19S Proteasomes Modulate Neuron-Glioma Synaptic Signaling. (A) Flow cytometric analysis of free 19S proteasomes (PSMD6 + PSMA7 - ) in EGFP + GL261 glioma cells under indicated conditions (n = 3). (B) Flow cytometric analysis of free 20S proteasomes (PSMA7 + PSMD6 - ) in EGFP + GL261 glioma cells under indicated conditions (n = 3). (C) Representative fluorescence microscopy images of CM-DiI-labeled GL261 cells (red) stained with membrane potential probe M09 (green) (n = 3). Scale bars: 100 μm. (D) Quantitative analysis of M09 MFI relative to control. Data normalized to monoculture group (set as 1.0) (n = 3). (E) Flow cytometric analysis of M09 signal in CM-DiI + GL261 cells under indicated conditions. (F) Percentage of M09-positive cells in CM-DiI + GL261 populations across treatment groups (n = 3). Data are means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: GL261 glioma cells were pre-labeled with CellTrackerTM CM-DiI (5 μM; MedChemExpress, HY-D1627) for 1 h at 37 °C to enable cellular identification prior to co-culture with HT22 neuronal cells for NGS establishment.

Techniques: Fluorescence, Microscopy, Labeling, Staining, Membrane, Control

Journal: bioRxiv

Article Title: Macrophage-specific NF-κB activation dynamics can segregate inflammatory bowel disease patients

doi: 10.1101/535096

Figure Lengend Snippet: Time-lapse Confocal imaging of NF-κB nuclear translocation in Lipid A-stimulated human PBMDMs. (A) PBMC-derived macrophages from human subjects were transduced with a fluorescent lentiviral p65AmCyan construct and were used to image NF-κB p65 subunit cellular dynamics in real-time. (B) CellTracker was used to generate profiles of nuclear translocation of transduced cells post stimulation with 200ng/mL Lipid A, over 3h: Asterisks (*) indicate nuclear localization of p65 at 66 and 93 min (‘). (C-E) Graphs of individual nuclear translocation profiles of responding cells in the healthy controls, Crohn’s disease (CD) and ulcerative colitis (UC) groups; mean ± SD for all responsive cells shown in blue, red and green colour lines for each group, respectively. (F) The proportion of responding cells was calculated by pooling all tracked cells from each disease group; *p<0.05; two-sided Fisher’s exact test (by summation). Peak identification and analysis revealed in each population the peak time (G), peak amplitude (H) and peak width (I): *p<0.05, ***p<0.001; statistical differences assessed by Kruskal Wallis test with pairwise comparison of all three groups.

Article Snippet: Cells were imaged using a Zeiss LSM880 confocal microscope system equipped with a cell incubation unit maintained at 37 ° C, in a humidified atmosphere of 5% CO 2 . p65-AmCyan nuclear fluorescence was detected (excitation λ 458nm, emission λ 489nm) and quantified using CellTracker software ( ).

Techniques: Imaging, Translocation Assay, Derivative Assay, Transduction, Construct, Comparison