cellbrite550 Search Results


cellbrite550  (Biotium)
94
Biotium cellbrite550
Ophiobolin A displays cancer cell-selective cytotoxicity via a lytic mechanism. ( A ) Cell viability of indicated cell lines treated with OpA at 500 nM for 24 h. The values presented are relative to the viability of vehicle-treated cells and normalized to 100%. Letters indicate statistically significant groupings via one-way ANOVA with Tukey’s correction for multiple hypothesis testing. ( B ) MDA-MB-231 cells were injected into the mammary fat pad of Scid/bg mice. Upon reaching 100 mm 3 , mice were treated with 5 mg/kg OpA or 5 mg/kg docetaxel i.p. weekly. Tumor growth curve assessed by caliper measurement. Tumor size data compared by two-way ANOVA with Sidak’s correction for multiple hypothesis testing. ( C – H ) Images and quantifications showing plasma membrane stain <t>(Cellbrite550)</t> and DNA stain (SYTOX green) in OpA (500 nM)-treated versus control cells for 6 h in indicated cell lines. Original magnification 20×; scale bar 10 µm. Cell size measured as area using ImageJ software (v1.54) and compared by Student’s t -test. ( I ) Confocal images of MDA-MB-231 cells treated with OpA (500 nM) showing membrane rupture and cell swelling compared to DMSO control. Original magnification 20×; scale bar 5 µm. All data are presented as the mean ± SD from at least three independent experiments. ** p < 0.01, *** p < 0.001, ns = not significant vs. control.
Cellbrite550, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellbrite550/product/Biotium
Average 94 stars, based on 1 article reviews
cellbrite550 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier

Image Search Results


Ophiobolin A displays cancer cell-selective cytotoxicity via a lytic mechanism. ( A ) Cell viability of indicated cell lines treated with OpA at 500 nM for 24 h. The values presented are relative to the viability of vehicle-treated cells and normalized to 100%. Letters indicate statistically significant groupings via one-way ANOVA with Tukey’s correction for multiple hypothesis testing. ( B ) MDA-MB-231 cells were injected into the mammary fat pad of Scid/bg mice. Upon reaching 100 mm 3 , mice were treated with 5 mg/kg OpA or 5 mg/kg docetaxel i.p. weekly. Tumor growth curve assessed by caliper measurement. Tumor size data compared by two-way ANOVA with Sidak’s correction for multiple hypothesis testing. ( C – H ) Images and quantifications showing plasma membrane stain (Cellbrite550) and DNA stain (SYTOX green) in OpA (500 nM)-treated versus control cells for 6 h in indicated cell lines. Original magnification 20×; scale bar 10 µm. Cell size measured as area using ImageJ software (v1.54) and compared by Student’s t -test. ( I ) Confocal images of MDA-MB-231 cells treated with OpA (500 nM) showing membrane rupture and cell swelling compared to DMSO control. Original magnification 20×; scale bar 5 µm. All data are presented as the mean ± SD from at least three independent experiments. ** p < 0.01, *** p < 0.001, ns = not significant vs. control.

Journal: International Journal of Molecular Sciences

Article Title: Gasdermin D Cleavage and Cytokine Release, Indicative of Pyroptotic Cell Death, Induced by Ophiobolin A in Breast Cancer Cell Lines

doi: 10.3390/ijms27020618

Figure Lengend Snippet: Ophiobolin A displays cancer cell-selective cytotoxicity via a lytic mechanism. ( A ) Cell viability of indicated cell lines treated with OpA at 500 nM for 24 h. The values presented are relative to the viability of vehicle-treated cells and normalized to 100%. Letters indicate statistically significant groupings via one-way ANOVA with Tukey’s correction for multiple hypothesis testing. ( B ) MDA-MB-231 cells were injected into the mammary fat pad of Scid/bg mice. Upon reaching 100 mm 3 , mice were treated with 5 mg/kg OpA or 5 mg/kg docetaxel i.p. weekly. Tumor growth curve assessed by caliper measurement. Tumor size data compared by two-way ANOVA with Sidak’s correction for multiple hypothesis testing. ( C – H ) Images and quantifications showing plasma membrane stain (Cellbrite550) and DNA stain (SYTOX green) in OpA (500 nM)-treated versus control cells for 6 h in indicated cell lines. Original magnification 20×; scale bar 10 µm. Cell size measured as area using ImageJ software (v1.54) and compared by Student’s t -test. ( I ) Confocal images of MDA-MB-231 cells treated with OpA (500 nM) showing membrane rupture and cell swelling compared to DMSO control. Original magnification 20×; scale bar 5 µm. All data are presented as the mean ± SD from at least three independent experiments. ** p < 0.01, *** p < 0.001, ns = not significant vs. control.

Article Snippet: To interrogate the formation of necrosomes, RIPK3-overexpressing cells were seeded in 96-well glass-bottom plates (Ref4580, Corning, Mediatech Inc., Manassas, VA, USA), treated with DMSO or OpA or TNF-α (YD370435, Thermo Scientific) and zVAD along with doxycycline (Cat# BP26535, Fisher Scientific) for 24 h. To examine the integrity of the plasma membrane, the cells were treated with or without OpA for 4 h and stained with Cellbrite550 (Cat#30105A, Biotium, Fremont, CA, USA) and SYTOX green (S7020, Fisher Scientific) in respective media for 30 min at 37 °C.

Techniques: Injection, Clinical Proteomics, Membrane, Staining, Control, Software