cell separation kit Search Results


cs 600 1 kit  (Cytiva Europe)
93
Cytiva Europe cs 600 1 kit
Cs 600 1 Kit, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/CS-600%2E1+Sepax+cell+separation+kit/us12378521-816-36-38
Average 93 stars, based on 1 article reviews
cs 600 1 kit - by Bioz Stars, 2026-10
93/100 stars
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g 100  (GE Healthcare)
93
GE Healthcare g 100
Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, <t>G-100,</t> or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.
G 100, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/CS-430%2E1+Sepax+cell+separation+kit/pmc07432554-231-34-12
Average 93 stars, based on 1 article reviews
g 100 - by Bioz Stars, 2026-10
93/100 stars
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excretory b  (Danaher Inc)
90
Danaher Inc excretory b
Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, <t>G-100,</t> or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.
Excretory B, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/CS-540%2E4b+Sepax+cell+separation+kit/pmc08882435-85-59-84
Average 90 stars, based on 1 article reviews
excretory b - by Bioz Stars, 2026-10
90/100 stars
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ti tr te 5 850 1000 8 3  (Danaher Inc)
90
Danaher Inc ti tr te 5 850 1000 8 3
Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, <t>G-100,</t> or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.
Ti Tr Te 5 850 1000 8 3, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/CS-900%2E2+Sepax+cell+separation+kit/pm11590641-45-20-13
Average 90 stars, based on 1 article reviews
ti tr te 5 850 1000 8 3 - by Bioz Stars, 2026-10
90/100 stars
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peripheral blood mononuclear cell separation kit  (Beijing Solarbio Science)
93
Beijing Solarbio Science peripheral blood mononuclear cell separation kit
Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, <t>G-100,</t> or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.
Peripheral Blood Mononuclear Cell Separation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/Fish+Peripheral+Blood+Mononuclear+Cell+Separation+Solution+Kit/pmc08481836-57-8-16
Average 93 stars, based on 1 article reviews
peripheral blood mononuclear cell separation kit - by Bioz Stars, 2026-10
93/100 stars
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ash 4 50 b 5 49  (Danaher Inc)
90
Danaher Inc ash 4 50 b 5 49
Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, <t>G-100,</t> or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.
Ash 4 50 B 5 49, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/CS-490%2E1+Sepax+cell+separation+kit/10__1080_slash_1828051x__2020__1712266-123-47-18
Average 90 stars, based on 1 article reviews
ash 4 50 b 5 49 - by Bioz Stars, 2026-10
90/100 stars
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anion exchange column  (Danaher Inc)
93
Danaher Inc anion exchange column
Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, <t>G-100,</t> or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.
Anion Exchange Column, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/CS-530%2E1+Sepax+cell+separation+kit/pm25287704-210-13-9
Average 93 stars, based on 1 article reviews
anion exchange column - by Bioz Stars, 2026-10
93/100 stars
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protein g sepharose beads  (Danaher Inc)
95
Danaher Inc protein g sepharose beads
Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, <t>G-100,</t> or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.
Protein G Sepharose Beads, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/CS-540%2E4+Sepax+cell+separation+kit/pmc03079418-122-10-13
Average 95 stars, based on 1 article reviews
protein g sepharose beads - by Bioz Stars, 2026-10
95/100 stars
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membrane cutoff  (Danaher Inc)
94
Danaher Inc membrane cutoff
Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, <t>G-100,</t> or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.
Membrane Cutoff, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/CS-570%2E4+Sepax+cell+separation+kit/pmc05800800-201-22-30
Average 94 stars, based on 1 article reviews
membrane cutoff - by Bioz Stars, 2026-10
94/100 stars
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aa 24 100  (Danaher Inc)
92
Danaher Inc aa 24 100
Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, <t>G-100,</t> or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.
Aa 24 100, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/CS-530%2E4+Sepax+cell+separation+kit/pmc02648312-199-16-41
Average 92 stars, based on 1 article reviews
aa 24 100 - by Bioz Stars, 2026-10
92/100 stars
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δt w l se  (Danaher Inc)
90
Danaher Inc δt w l se
Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, <t>G-100,</t> or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.
δt W L Se, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/CS-530%2E4b+Sepax+cell+separation+kit/10__2147_slash_ijn__s202049-160-7-29
Average 90 stars, based on 1 article reviews
δt w l se - by Bioz Stars, 2026-10
90/100 stars
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vpsp  (Danaher Inc)
86
Danaher Inc vpsp
Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, <t>G-100,</t> or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.
Vpsp, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, G-100, or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.

Journal: International Journal of Molecular Sciences

Article Title: Development of Novel High-Resolution Size-Guided Turbidimetry-Enabled Particle Purification Liquid Chromatography (PPLC): Extracellular Vesicles and Membraneless Condensates in Focus

doi: 10.3390/ijms21155361

Figure Lengend Snippet: Columns packed with gradient size exclusion chromatography (gSEC) beads achieve higher resolution as compared to monosize bead columns. ( a , b ) Schematic describing that monosize bead separation ( a ) would end up with only two peaks, whereas separation with gSEC beads ( b ) may achieve a multi-peak resolution. ( c ) Schematic of the workflow for the clarification of crude extracellular vesicle (EV) mixtures from body fluids and cell culture supernatants by differential centrifugation before column separation. ( d ) Seminal plasma from 6 healthy men were pooled and clarified, and equal volumes (1 mL) were run on 50 cm × 0.5 cm G-10, G-100, or gSEC Sephadex bead-packed columns. Elution was carried out with phosphate-buffered saline (PBS), fractions were collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. The profiles shown correspond to the 280-nm wavelength. ( e ) Gradient separation profiles of seminal plasma on columns of different lengths. Aliquots (1.5 mL) from the same pool of clarified seminal plasma (healthy, n = 6) were loaded on different-length Econo ® columns (20, 50, and 100 cm) packed with multi-size beads, with the elution and collection carried out under the same conditions. ( f – j ) Samples were purified on a 100 × 1 cm gSEC column. Fractions were eluted with PBS, collected in 96-well plates, and the UV-Vis absorbance measurements were recorded using a plate reader. ( f ) Seminal plasma separation profiles (1.5 mL) from 4 individual healthy donors. ( g ) Blood plasma and serum separation profiles. Blood was collected from 4 donors in different collection tubes, and clarified plasmas and serums were pooled by tube type. Three-hundred microliters of each pool were used for separation. ( h ) Forty milliliters of a first-void clean-catch urine sample was collected from a healthy male, clarified, and concentrated 10 times to 4 mL (Amicon™ ultra centrifugal filter unit, 3000 Da)—of which, 1.5 mL was used for purification. ( i ) Commercial cow milk separation profiles. Whole, 2% fat, and fat-free cow milk were purchased from Walmart, clarified by centrifugation at 10,000× g for 30 min, and 1 mL of each clarified sample was used. ( j ) U1, 293T, and MDA-MB-231 cells were cultured in 150 × 20 mm dishes in media supplemented with 10% exosome-depleted fetal bovine serum (FBS) for 3 days, and 10 mL of supernatants were clarified by differential centrifugation and concentrated (Amicon™ ultra-centrifugal filter unit, 3000 Da) to 1 mL before purification. All profiles shown correspond to the 280-nm wavelength. RT: room temperature.

Article Snippet: All available size-exclusion dextran-based Sephadex TM beads were purchased from Cytiva (formely GE Healthcare, Marlborough, MA, USA) and were as follows: G-10 (17-0010-01), G-15 (170020-01), G-25 fine (17-0032-01), G-50 fine (17-0042-01), G-75 (17-0050-01), and G-100 (17-0060-01). gSEC column was prepared by layering the beads from the smallest to the largest in different Econo-Columns ® (Bio-Rad, Hercules, CA, USA) at room temperature by gravity.

Techniques: Size-exclusion Chromatography, Clarification Assay, Cell Culture, Centrifugation, Purification