Journal: Cell Death & Disease

Article Title: Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment

doi: 10.1038/cddis.2013.88

Figure Lengend Snippet: Characterization of HS-181 cell line after treatment with zebularine. ( a ) RT-PCR of cardiac markers after treatment with zebularine. Myh7, Myh6, Actc, cTnI and Serca2 present more expression after treatment. ( b ) Immunostaining of treated cells to detect cardiac-specific proteins. Scale bars; 50 μ m. ( c ) Blots of Proteome Profiler Array and the resulting quantification histograms demonstrating inhibition of pluripotency marker expression and ( d ) increased levels of mesodermic proteins after zebularine treatment (black arrows)

Article Snippet: Protein expression profiles were assayed using the specific human pluripotent Stem Cell array kit ‘Proteome Profiler Array' (R&D Systems Europe, Abingdon, UK; ARY010) following the manufacturer's instructions.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Immunostaining, Inhibition, Marker

Journal: Cell Stress & Chaperones

Article Title: Hypoxia- and mechanical stress–induced upregulation of mitochondrial HSP60 is associated with phenotypic switching of pulmonary arterial smooth muscle cells

doi: 10.1016/j.cstres.2025.100089

Figure Lengend Snippet: Inflammatory cytokine levels in supernatant of PASMCs cultured under different conditions . Detection of inflammatory cytokines was performed by Proteome Profiler™ Human Cytokine Array. Representative array images (left) and semiquantitative data (right) are shown.

Article Snippet: Human cell stress proteins were subsequently detected using the Proteome Profiler Human Cell Stress Array Kit (#ARY018, R&D Systems, Minneapolis, USA), following the manufacturer's instructions.

Techniques: Cell Culture