Journal: Molecules

Article Title: Antifungal Properties of Essential Oils and Their Compounds for Application in Skin Fungal Infections: Conventional and Nonconventional Approaches

doi: 10.3390/molecules26041093

Figure Lengend Snippet: Potential of EOs as antifungal agents in human skin disease.

Article Snippet: 24. , In vitro—Conventional technology , [ ] , Skin diseases ( Fusarium oxysporum , T. rubrum and T. mentagrophytes ) , Seventeen EOs , EOs analysis by GC-MS and GC-FID. Antifungal activity by M38-A protocol. Cytotoxicity assay using MTT technique. , Strong activity was demonstrated in 70% to 80% of samples against dermatophytes and from 20% against F. oxysporum . The lowest MIC values were obtained with citral chemotype Lippia alba oil (BC2) at concentrations of 31.25 and 125 μg/mL on T. rubrum and T. mentagrophytes , respectively, but not against F. oxysporum. Oil from Minthostachys mollis (Kunth) Griseb showed strong activity against all fungi evaluated. Active samples against dermatophytes and F. oxysporum were not cytotoxic on vero cells ATCC CCL-81; excluding Lippia origanoides Kunth (5E), carvone chemotype Lippia alba (TS) and M. mollis oil (MEO2) oil. The EOs with the highest selectivity index (SI) values were Aloysia triphylla and L. alba oil (BC2) on dermatophytes. The main components of most active L. alba oils were characterized as carvone (TS, CC1) and citral (BC2). To L. origanoides oils, carvacrol (1A, 5E) and thymol (6F) were found as the main components. Pulegone and cis-piperitone epoxide were the main constituents of M. mollis MEO1 and MEO2 oils, respectively , The main components in these EOs may be responsible for their antifungal activity. This finding is very important, because it confirms the potential of these EOs as a source of new antidermatophytes..

Techniques: Gas Chromatography-Mass Spectrometry, Single Cell Gel Electrophoresis, Activity Assay, In Vitro, Chromatography, Microdilution Assay, Infection, Isolation, Diffusion-based Assay, Control, MTT Assay, Dilution Assay, In Vivo, Inhibition, Ointment, Concentration Assay, Drug discovery, Cytotoxicity Assay, Viscosity, Bacteria, Formulation, Modification, Sterility, Transformation Assay, Spectroscopy, Histopathology, Functional Assay, Positive Control, Serial Dilution, Emulsion, Solvent, Evaporation, Injection, Biomarker Discovery, Clinical Proteomics, Adhesive

Journal: Molecules

Article Title: Antifungal Properties of Essential Oils and Their Compounds for Application in Skin Fungal Infections: Conventional and Nonconventional Approaches

doi: 10.3390/molecules26041093

Figure Lengend Snippet: Potential of EOs as antifungal agents in human skin disease.

Article Snippet: 24. , In vitro—Conventional technology , [ ] , Skin diseases ( Fusarium oxysporum , T. rubrum and T. mentagrophytes ) , Seventeen EOs , EOs analysis by GC-MS and GC-FID. Antifungal activity by M38-A protocol. Cytotoxicity assay using MTT technique. , Strong activity was demonstrated in 70% to 80% of samples against dermatophytes and from 20% against F. oxysporum . The lowest MIC values were obtained with citral chemotype Lippia alba oil (BC2) at concentrations of 31.25 and 125 μg/mL on T. rubrum and T. mentagrophytes , respectively, but not against F. oxysporum. Oil from Minthostachys mollis (Kunth) Griseb showed strong activity against all fungi evaluated. Active samples against dermatophytes and F. oxysporum were not cytotoxic on vero cells ATCC CCL-81; excluding Lippia origanoides Kunth (5E), carvone chemotype Lippia alba (TS) and M. mollis oil (MEO2) oil. The EOs with the highest selectivity index (SI) values were Aloysia triphylla and L. alba oil (BC2) on dermatophytes. The main components of most active L. alba oils were characterized as carvone (TS, CC1) and citral (BC2). To L. origanoides oils, carvacrol (1A, 5E) and thymol (6F) were found as the main components. Pulegone and cis-piperitone epoxide were the main constituents of M. mollis MEO1 and MEO2 oils, respectively , The main components in these EOs may be responsible for their antifungal activity. This finding is very important, because it confirms the potential of these EOs as a source of new antidermatophytes..

Techniques: Gas Chromatography-Mass Spectrometry, Single Cell Gel Electrophoresis, Activity Assay, In Vitro, Chromatography, Microdilution Assay, Infection, Isolation, Diffusion-based Assay, Control, MTT Assay, Dilution Assay, In Vivo, Inhibition, Ointment, Concentration Assay, Drug discovery, Cytotoxicity Assay, Viscosity, Bacteria, Formulation, Modification, Sterility, Transformation Assay, Spectroscopy, Histopathology, Functional Assay, Positive Control, Serial Dilution, Emulsion, Solvent, Evaporation, Injection, Biomarker Discovery, Clinical Proteomics, Adhesive