Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: Adipocyte

Article Title: Optimization of in vitro cultivation strategies for human adipocyte derived stem cells

doi: 10.4161/21623945.2014.987580

Figure Lengend Snippet: (A) Cells cultured in BM 2 (right) show a higher proliferation rate during a 24-h cultivation period than cells cultivated in BM 1 (left). Each value represents the mean ± SD of triplicate values from 4 different ADSC-Preparations. Absorbance values obtained with medium alone (<0.02) were taken as the control and subsequently subtracted from all measurements. (B) Microscopic examination of ADSC cultured in 2 different culture media. 4 representative phase-contrast images are shown. left: ADSC were seeded at a density of 10.000 in DMEM/F12 medium (BM1) in 10 cm2 round tissue culture dishes and photographed 24 and 48 hours after seeding. Right: ADSC were seeded at a density of 10.000 in ECGM medium (BM2) in 10 cm2 round tissue culture dishes and photographed 24 and 48 hours after seeding.

Article Snippet: The isolated preadipocytes were cultured both in BM 1 as described above, and in a specialized endothelial cell growth medium (ECGM), BM2, which was supplied by Promocell and whose exact content will remain undisclosed because of the patent rights of the manufacturer.

Techniques: Cell Culture

Journal: Adipocyte

Article Title: Optimization of in vitro cultivation strategies for human adipocyte derived stem cells

doi: 10.4161/21623945.2014.987580

Figure Lengend Snippet: Quantification of Oil-red O positive surface areas representing the amount of mature Adipocytes. One representative example of ADCS submitted to either BM1 (A) or BM2 medium (B) simultaneously and subsequently differentiated using induction and differentiation regimen is shown. BM1 shows a coverage of 0.9 % in comparison to 12.1% with BM 2.

Article Snippet: The isolated preadipocytes were cultured both in BM 1 as described above, and in a specialized endothelial cell growth medium (ECGM), BM2, which was supplied by Promocell and whose exact content will remain undisclosed because of the patent rights of the manufacturer.

Techniques: