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Image Search Results
Journal: Nature Communications
Article Title: Tripartite motif containing 26 prevents steatohepatitis progression by suppressing C/EBPδ signalling activation
doi: 10.1038/s41467-023-42040-9
Figure Lengend Snippet: a Schematic of the experimental procedure showing the genes expression in L02 cells after adenovirus-mediated TRIM26 overactivation (Ad TRIM26 ). b – g Volcano plot; average protein expression ratio of 3 replicates (log 2 transformed) between 0.5 mM palmitate+1.0 mM oleic acid (PAOA)-induced AdGFP- or Ad TRIM26 -transfected L02 cells was plotted against p -value by Student’ s t test (−log 10 transformed). Cutoff of P < 0.05 and 1.2-fold change were marked by black dotted lines, respectively. The figure shows the total number of proteins identified as well as the number of up- and downregulated proteins ( b ), principal component analysis (PCA) analysis ( c ), heatmap ( d ), KEGG pathway & wiki-pathway analysis ( e ), Sankey diagram enrichment ( f ) and GO biological process analysis; data was plotted against p -value by Student’ s t test (−log 10 transformed) with cutoff of P < 0.05 and 1.2-fold change ( g ) in AdGFP-transfected or Ad TRIM26 -transfected L02 cells with PAOA co-treatment showing that TRIM26 affects lipid metabolism and inflammatory responses. h Circle diagram analysed by GeneMANIA further showing TRIM26-CEBPD protein interaction network in Homo sapiens and Mus musculus .
Article Snippet: Homo sapiens full-length TRIM26 sequences and designed short hairpin RNA (shRNA) targeting human TRIM26 (sh TRIM26 ) (shRNA sequences RNA-1: GATGGATATGACGACTGGGAA; RNA-2: GATGGATATGACGACTGGGAA), adenovirus expressing shRNA for silencing of Mus musculus Trim26 (Cat. No: shADV-274978, Vector Biolabs, Malvern, PA), human WT TRIM26 sequences with AXXA and AXXXXA motifs of RING domain mutation, Homo sapiens full-length CEBPD sequences, and adenovirus expressing shRNA for silencing of
Techniques: Expressing, Transformation Assay, Transfection
Journal: PLoS ONE
Article Title: Liver-Derived IGF-I Regulates Mean Life Span in Mice
doi: 10.1371/journal.pone.0022640
Figure Lengend Snippet: Genes regulated in liver, skeletal muscle, heart, fat (retroperitoneal fat), and bone (vertebrae) in 7-month-old female control (n = 6) and LI-IGF-I -/- (n = 7) mice as determined by DNA microarray analyses.
Article Snippet: We used FAM-labeled probes specific for heat shock protein 1A (Mm01159846_s1) and 1B (Mm03038954_s1), connective tissue growth factor (Mm01192931_g1), CCAAT/enhancer protein delta (
Techniques: Control, Microarray
Journal: Cell reports
Article Title: Mapping Distinct Bone Marrow Niche Populations and Their Differentiation Paths
doi: 10.1016/j.celrep.2019.06.031
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Recombinant, shRNA, Construct, Plasmid Preparation, Sequencing, Software