Journal: Nature Immunology

Article Title: Antigen specificity of clonally enriched CD8 + T cells in multiple sclerosis

doi: 10.1038/s41590-025-02412-3

Figure Lengend Snippet: a , Summary of EBV DNA ddPCR results from CSF supernatant in which EBER2 was normalized to a housekeeping gene (MS/CIS, n = 13; HC/OND, n = 5). b , EBV cDNA for each of the indicated genes were measured by ddPCR and normalized to a housekeeping gene. Each sample was run in duplicate and each dot represents the average result from each study participant. Data are the mean ± s.e.m. MS/CIS and HC/OND samples were compared using an unpaired two-tailed Student’s t -test with Welch’s correction; NS, not significant; n = 13 for MS/CIS for all genes except EBER2 where n = 12 due to lack of sufficient sample for MS27 and n = 5 in HC/OND for all genes except BamHI-W where n = 4 due to a lack of sufficient sample for OND4.

Article Snippet: Complementary DNA was synthesized using a ProtoScript first strand cDNA synthesis kit (NEB) using 6 μl RNA per 20 μl reaction volume according to the manufacturer’s instructions.

Techniques: Two Tailed Test