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Image Search Results
Journal: PLoS Pathogens
Article Title: RNA-seq Brings New Insights to the Intra-Macrophage Transcriptome of Salmonella Typhimurium
doi: 10.1371/journal.ppat.1005262
Figure Lengend Snippet: S . Typhimurium strain 4/74 was grown within macrophages for 8 hours using the gentamicin protection assay, and bacterial RNA was isolated using TRIzol (Materials and Methods). The cDNA generated from total RNA was sequenced either directly for gene/sRNA expression analysis (RNA-seq) or after enrichment of primary transcripts (dRNA-seq), and compared with data from 4/74 grown to ESP . The graphs show representations of sequence reads mapped uniquely against the 4/74 genome in different conditions. Transcript per Million (TPM) analysis was used to calculate gene expression values from the number of sequence reads mapped against the 4/74 genome. The promoter usage value (PUV) indicates the TPM value of the first 10 nucleotides from the transcription start sites (TSS) in the direction of transcription, and represents promoter strength. Each curved arrow indicates location of TSS upstream of the respective gene; the width and height of each curved arrow is proportional to TSS expression, based on relative PUV, macrophage versus ESP.
Article Snippet: The
Techniques: Isolation, Generated, Expressing, RNA Sequencing, Sequencing, Gene Expression