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Image Search Results
Journal: Scientific Reports
Article Title: Caldesmon-1–mediated actin dynamics is essential for osteogenic differentiation of aortic valve interstitial cells
doi: 10.1038/s41598-026-39938-x
Figure Lengend Snippet: Localisation of caldesmon-1 (CALD1) and α-smooth muscle actin (αSMA) proteins in normal and AS aortic valves. ( a ) Immunohistochemical staining with anti-CALD1 and anti-αSMA antibodies and histological analyses by haematoxylin and eosin (H&E) and Massons’ trichrome in normal and aortic valve stenosis (AS) valve leaflets. Scale bar = 100 μm. ( b ) Quantification of CALD1- and αSMA-positive areas in normal and AS valves ( n = 7). Statistical significance was determined using a Mann–Whitney U test (* P < 0.05). ( c ) Enlarged image of the CALD1 IHC shown in Fig. 1a. Calcified areas identified histologically are highlighted in red ( Left panel ). Immunofluorescence images of CALD1- (green) and αSMA- (red) double-positive VICs in AS valve (Non-calcified area and Calcified area) ( Right panel ). Scale bar = 50 μm.
Article Snippet: Membranes were incubated with primary antibodies, including
Techniques: Immunohistochemical staining, Staining, MANN-WHITNEY, Immunofluorescence