Journal: RSC Advances

Article Title: Discovery of new Hsp90–Cdc37 protein–protein interaction inhibitors: in silico screening and optimization of anticancer activity †

doi: 10.1039/d4ra05878j

Figure Lengend Snippet: Representative natural products and celastrol derivative 41, withaferin A, FW-04-806 and rationally designed small molecules DCZ3112 and DDO-5936, which prevent the formation of the Hsp90–Cdc37 PPI. Blocking the interaction between Hsp90 (PDB ID: 8EOB ) and Cdc37 (PDB ID: 2K5B ) prevents the loading of Cdc37 clients such as Cdk4 (PDB ID: 2W96 ) to Hsp90 and thus the formation of the Hsp90-Cdk4-Cdc37 (PDB ID: 5FWP ) complex.

Article Snippet: Next, Cdc37 (AR09008PUN, OriGene Technologies, Inc., Rockville, MD, USA) was acquired for the purpose of PPI inhibition evaluation between full-length Hsp90β and Cdc37.

Techniques: Blocking Assay

Journal: iScience

Article Title: MOTS-c modulates skeletal muscle function by directly binding and activating CK2

doi: 10.1016/j.isci.2024.111212

Figure Lengend Snippet:

Article Snippet: Phospho-CDC37 (Ser13) antibody , Cell Signaling Technology , 13248; RRID: AB_2783724.

Techniques: Recombinant, RNA Sequencing, Software, Immunoprecipitation, Blocking Assay, Western Blot, Bicinchoninic Acid Protein Assay

Journal: The Journal of Biological Chemistry

Article Title: Chronic treatment with the complex I inhibitor MPP + depletes endogenous PTEN-induced kinase 1 (PINK1) via up-regulation of Bcl-2–associated athanogene 6 (BAG6)

doi: 10.1074/jbc.RA119.010474

Figure Lengend Snippet: List of antibodies used in the study

Article Snippet: Densitometry data were normalized to loading control, and the half-lives of PINK1 isoforms were estimated by fitting a one-phase exponential decay curve using Prism 8 software (GraphPad, San Diego, CA). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Species Catalog number Source Dilution PINK1 Rabbit BC100-494 Novus Biological 1:1000 TOM20 Rabbit Sc11415 Santa Cruz 1:10,000 β-ACTIN Mouse A5316 Sigma 1:2000 HSP60 Mouse 611563 BD Transduction 1:1000 BAG6 Mouse Sc365928 Santa Cruz 1:1000 BAG5 Rabbit NB100-56091 Novus Biological 1:1000 BAG2 Mouse Sc101216 Santa Cruz 1:1000 HSP90 Rabbit 4877 Cell Signaling 1:1000 CDC37 Rabbit 3604 Cell Signaling 1:1000 CHIP Rabbit Sc66830 Santa Cruz 1:1000 GAPDH Rabbit Ab37168 Abcam 1:10,000 GFP Rabbit A6455 Invitrogen 1:10,000 V5 Mouse Sc271944 Santa Cruz 1:1000 FLAG Rabbit F7425 Sigma 1:1000 β-Tubulin Rabbit Ab6046 Abcam 1:5000 Open in a separate window List of antibodies used in the study

Techniques: Transduction

Journal: Cancers

Article Title: On the Mechanism of Hyperthermia-Induced BRCA2 Protein Degradation

doi: 10.3390/cancers11010097

Figure Lengend Snippet: List of antibodies.

Article Snippet: Rabbit , CDC37 (3618S) , WB 1:1000 , Cell Signaling.

Techniques: