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Image Search Results
Journal: Stem Cells International
Article Title: Dot1l Regulates the Spontaneous Bone Regeneration of Periosteum-Derived Stem Cells by Regulating Chac1 Expression
doi: 10.1155/sci/1508850
Figure Lengend Snippet: Mandibular defect repair and periosteal PDSCs isolation: (A) animal experimental design, (B) surgical image of a rat model of jaw defect with periosteum preserved, (C) X-ray images of the defect area in rats at day 7 and 8 weeks later, (D) morphologic photographs of periosteum-derived stem cells in sham and model rats, (E) the proportions of CD44+, CD90+, CD31+, and CD45+ cells in PDSCs isolated from the jaws of sham-operated rats were analyzed using flow cytometry, and (F) the proportion of CD44+, CD90+, CD31+, and CD45+ cells in PDSCs isolated from jaws of model rats were analyzed using flow cytometry.
Article Snippet: For the identification of PDSCs cells, the cells were washed and incubated with CD44 antibody (15675-1-AP, Proteintech),
Techniques: Isolation, Derivative Assay, Flow Cytometry
Journal: Stem Cells International
Article Title: Dot1l Regulates the Spontaneous Bone Regeneration of Periosteum-Derived Stem Cells by Regulating Chac1 Expression
doi: 10.1155/sci/1508850
Figure Lengend Snippet: Expression pattern of Dot1l during mandibular defect healing, and Dot1l is important for the osteogenesis of PDSCs. (A) The volcano plot showed the differential genes in PDSCs isolated from the sham operation group and the periosteum-preserved jaw defect model group. (B) GO functional enrichment analysis showing the top 30 significantly enriched biological functions and roles. (C) The mRNA expression level of differential genes was detected using RT-qPCR assay, data were analyzed by using one-way ANOVA, with the Tukey's post hoc test. ∗∗∗∗ p < 0.001. (D) The protein expression level of Dot1l was detected using western blotting assay. (E) The proportion of CD44, CD90, CD31, and CD45 positive expression cells in PDSCs from 4-week-old SD rats was analyzed by flow cytometry. (F) ARS staining was used to detect calcium deposition in PDSCs cells, ALP staining was used to detect ALP activity of PDSCs cells, oil red O staining was used to detect the lipogenic ability of PDSCs cells. (G) Detection of the Dot1l mRNA in PDSCs treated with Dot1l ctrl/overexpression via RT-qPCR assay, data were analyzed by t -test analysis, ∗∗∗ p < 0.001. (H) Detection of the Dot1l mRNA in PDSCs treated with Dot1l ctrl/overexpression via western blot assay. (I) Detection of the mRNA expressions of osteogenic differentiation related genes in PDSCs treated with Dot1l ctrl/overexpression via RT-qPCR assay, data were analyzed by using one-way ANOVA, with the Tukey's post hoc test. ∗∗∗∗ p < 0.001. (J) ALP staining was used to detect ALP activity of PDSCs cells treated with Dot1l ctrl/overexpression. (K) ARS staining was used to detect calcium deposition of PDSCs cells treated with Dot1l ctrl/overexpression.
Article Snippet: For the identification of PDSCs cells, the cells were washed and incubated with CD44 antibody (15675-1-AP, Proteintech),
Techniques: Expressing, Isolation, Functional Assay, Quantitative RT-PCR, Western Blot, Flow Cytometry, Staining, Activity Assay, Over Expression