Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells

doi: 10.3389/fbioe.2019.00321

Figure Lengend Snippet: Cell proliferation capacity in human IPFSCs after FN1-KO. FN1-KO cells were compared with copGFP in cell increase (A) , percentage of cells in the S and G 2 phases (B) , and surface markers [SSEA4 (C) , CD73 (D) , CD90 (E) , CD105 (F) , and CD146 (G) ] by flow cytometry; stemness genes ( NANOG, SOX2, KLF4, BMI1, MYC, NOV, POU5F1 , and NES ) (H) , senescent genes ( CDKN1A, CDKN2A , and TP53 ) (I) , and the mesenchymal condensation gene ( CDH2 ) (J) by qPCR. GAPDH was used as an endogenous control. Data are shown as bar charts. * indicates a significant difference compared to the corresponding copGFP group ( P < 0.05).

Article Snippet: The following primary antibodies were used: CD73-APC (cat no. 17-0739-42; eBioScience, Fisher Scientific, Waltham, MA), CD90-APC-Vio770 (cat no. 130-114-863; Miltenyi Biotec, San Diego, CA), CD105-PerCP-Vio700 (cat no. 130-112-170; Miltenyi Biotec), CD146-PE (cat no. 12-1469-42; eBioScience), and the stage-specific embryonic antigen 4-PE (SSEA4-PE; cat no. 330406; BioLegend, Dedham, MA).

Techniques: Flow Cytometry, Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells

doi: 10.3389/fbioe.2019.00321

Figure Lengend Snippet: Proliferation capacity of human IPFSCs after expansion on the dECMs deposited by FN1-KO cells. Passage 15 human IPFSCs were compared after expansion on dECMs deposited by Cas9-sgFN1a/b transduced cells (sgFN1a ECM and sgFN1b ECM, respectively) with those deposited by copGFP (copGFP ECM) and those grown on TCP (TCP) as controls in cell increase (A) , percentage of cells in the S and G 2 phases (B) , and surface markers [SSEA4 (C) , CD73 (D) , CD90 (E) , and CD105 (F) ] by flow cytometry; stemness genes ( NANOG, SOX2, KLF4, BMI1, MYC, NOV, POU5F1 , and NES ) (G) , senescence genes ( CDKN1A, CDKN2A , and TP53 ) (H) , and the mesenchymal condensation gene ( CDH2 ) (I) by qPCR. GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group ( P < 0.05).

Article Snippet: The following primary antibodies were used: CD73-APC (cat no. 17-0739-42; eBioScience, Fisher Scientific, Waltham, MA), CD90-APC-Vio770 (cat no. 130-114-863; Miltenyi Biotec, San Diego, CA), CD105-PerCP-Vio700 (cat no. 130-112-170; Miltenyi Biotec), CD146-PE (cat no. 12-1469-42; eBioScience), and the stage-specific embryonic antigen 4-PE (SSEA4-PE; cat no. 330406; BioLegend, Dedham, MA).

Techniques: Flow Cytometry, Control

Journal: Biotechnology and bioengineering

Article Title: Mesenchymal stem cells and local tacrolimus delivery synergistically enhance neurite extension

doi: 10.1002/bit.27916

Figure Lengend Snippet: Presence and verification of stem cells is visualized after seeding with mesenchymal stem cells (MSC; 2-IB, 2-IIB), treatment with 100 ng/mL tacrolimus (FK506; 2-IC, 2-IIC), or MSCs and 100 ng/mL FK506 combined (2-ID, 2-IID), and compared to control (2-IA, 2-IIA; no treatment). Neurites grew from the DRG into the nerve graft: from the circular top to bottom, respectively. Presence of the CD90 marker (green) in both autograft groups and allograft groups was evident on the nerve grafts seeded with MSCs and nerve grafts with combined treatment (respectively, groups B and D).

Article Snippet: After CD90 staining (monoclonal mouse anti rat 1:100, Bio-Rad, Hercules, CA, USA), sections are incubated at RT for 2 hours with DAPI (4’,6- Diamidino-2-Phenylindole, Dihydrochloride) (1:1000, Thermo Fisher Scientific).

Techniques: Control, Marker

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: Summary of the 15 antibodies used for the mass cytometry analysis.

Article Snippet: Anti-Human CD90 , 159Tb , Fluidigm , 3159007B , AB_2893063.

Techniques: Mass Cytometry

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: CyTOF single-cell analysis of surface antigens in PDHGG patient-derived cell lines. t-SNE maps showing the expression of 10 surface markers (CD31, CD34, CD63, CD36, CD29, CD90, CD140a, CD49c, CD56 and CD61) in each of the eight different PDHGG patient-derived cell lines analyzed through mass cytometry technique. The color gradient refers to the intensity of the expression of the considered marker, in a blue to red scale indicating low and high intensity respectively.

Article Snippet: Anti-Human CD90 , 159Tb , Fluidigm , 3159007B , AB_2893063.

Techniques: Single-cell Analysis, Derivative Assay, Expressing, Mass Cytometry, Marker

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: List of the cell antigens used together with the molecular function and reported expression.

Article Snippet: Anti-Human CD90 , 159Tb , Fluidigm , 3159007B , AB_2893063.

Techniques: Expressing, Migration, Mutagenesis