Journal: Nature Communications

Article Title: Differential expression of tissue-restricted antigens among mTEC is associated with distinct autoreactive T cell fates

doi: 10.1038/s41467-020-17544-3

Figure Lengend Snippet: Littermate control (LMC), CRP, and INS2 Tg mice (CD45.2, Thy1.2) were irradiated and reconstituted with 1% OT-II (CD45.2, Thy1.1) and 99% WT (CD45.1, Thy1.2) bone marrow. The hematopoietic chimeras were analyzed 6-8 weeks post-irradiation. a Proportion of mature TCRβ hi CD4 + SP OT-II thymocytes among total live cells and absolute number of live CD4 + SP OT-II thymocytes. b Proportion of OT-II cells within the CD4 + T cell population in the peripheral lymph nodes. c Representative flow plots of CD25 and FoxP3 expression on OT-II T cells. d Proportion and absolute cell number of CD25 + Foxp3 + OT-II T cells in the thymus. e Proportion of CD25 + Foxp3 + OT-II T cells in the lymph nodes. (mean ± SEM, n = 17 LMC, 8 CRP, 10 INS2, from three independent experiments). All p -values indicated are calculated by Kruskal-Wallis test followed by Dunn’s post-hoc comparisons to LMC, two-sided. ** p < 0.01 relative to LMC. Source data are provided as a Source Data file.

Article Snippet: Six to twelve week-old male or female donor mice were injected i.p. with 100 μg InVivoMAb anti-mouse Thy1 (BioXcell) at day −2 and −1 prior to harvesting the bone marrow cells.

Techniques: Control, Irradiation, Expressing

Journal: Cell and Tissue Research

Article Title: Acute myocardial infarction does not affect functional characteristics of adipose-derived stem cells in rats, but reduces the number of stem cells in adipose tissue

doi: 10.1007/s00441-015-2239-z

Figure Lengend Snippet: Effect of AMI on rat SVF cells. a Cell size (μm) of the SVF cells, demonstrating no difference between the three groups. b Percentage of colony-forming cells, showing significantly fewer colonies in the 1D group (6.1 ± 1.6 %) compared to the Control (11.4 ± 1.8 %) and 7D groups (11.0 ± 0.9 %). c Number of cells, obtained 4 days after seeding of the SVF fraction, demonstrating significantly fewer cells in the 1D group (1.3 ± 0.1 million cells) compared to the Control (2.0 ± 0.1 million cells) and 7D groups (1.8 ± 0.1 million cells). d Percentage of positive cells for the markers CD31, CD34, CD45, CD73, CD90, CD105 and CD271 showing significantly fewer CD90- and CD105-positive cells in the 1D group. Data shown as mean ± SD (Control and 7D group n = 6, 1D group n = 5, * p < 0.05, ** p < 0.01)

Article Snippet: The following primary antibodies were used: phycoerythrin (PE)-conjugated mouse-α-rat antibodies against CD90 (1:20; Cedarlane Laboratories, Ontario, Canada), CD45 (1:20; BD Biosciences, San José, CA, USA), CD271 (1:25; BD Biosciences) and CD31 (1:20; AbD Serotec, Oxford, UK), PE-labeled mouse α-human antibody CD105 (1:25; Caltag, Invitrogen, USA), as well as unlabeled mouse-α-rat antibodies against CD34 (1:20; Santa Cruz Biotechnology) and CD73 (1:20; BD Biosciences).

Techniques: Control

Journal: Cell and Tissue Research

Article Title: Acute myocardial infarction does not affect functional characteristics of adipose-derived stem cells in rats, but reduces the number of stem cells in adipose tissue

doi: 10.1007/s00441-015-2239-z

Figure Lengend Snippet: Effect of AMI on cultured rat ASC. a Representative light microscopy image demonstrating the morphology of cultured rat ASC. b Cell size (μm) of ASC, demonstrating no significant differences between the three groups. c Population doubling time (in days) depicted for passages 1–3, showing no significant differences between the three groups. d Percentage of positive cells for the markers CD31, CD34, CD45, CD73, CD90, CD105 and CD271, showing no significant differences in numbers of positive cells between the groups. Data shown as mean ± SD (Control and 7D group n = 6, 1D group n = 5)

Article Snippet: The following primary antibodies were used: phycoerythrin (PE)-conjugated mouse-α-rat antibodies against CD90 (1:20; Cedarlane Laboratories, Ontario, Canada), CD45 (1:20; BD Biosciences, San José, CA, USA), CD271 (1:25; BD Biosciences) and CD31 (1:20; AbD Serotec, Oxford, UK), PE-labeled mouse α-human antibody CD105 (1:25; Caltag, Invitrogen, USA), as well as unlabeled mouse-α-rat antibodies against CD34 (1:20; Santa Cruz Biotechnology) and CD73 (1:20; BD Biosciences).

Techniques: Cell Culture, Light Microscopy, Control