cd86 pe 65165 Search Results


cd86 antibody  (Proteintech)
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Proteintech cd86 antibody
Effect of TAMs phenotype on immunologic efficacy in EC patients and proliferation and migration for Ishikawa cells. A Immunohistochemistry (IHC) staining of M1-like TAMs <t>(CD86</t> + ) and M2-like TAMs (CD206 + ) in tumor tissues from EC patients. The bar graphs on the right show the IHC staining scores (*** P ≤ 0.001). B Flow cytometry analysis of M1-like TAMs (CD86 + ) and M2-like TAMs (CD206 + ) in PBMCs. The left panel shows representative flow plots, and the bar graphs on the right quantify the percentages (*** P ≤ 0.001). C Experimental design for TAMs polarization and co-culture system. D CCK-8 assay measuring cell viability of Ishikawa cells co-cultured with M1 and M2-like TAMs for 24, 48 and 72 h. The bar graphs show the percentage of viable cells (* P < 0.05, ** P ≤ 0.01). E Transwell migration assay of Ishikawa cells co-cultured with M1 and M2-like TAMs. The bar graphs on the right quantify the number of migrated cells (* P < 0.05, ** P ≤ 0.01)
Cd86 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd86+pe+65165/PE+Anti-human+CD86/pmc12181619-113-19-22
Average 93 stars, based on 1 article reviews
cd86 antibody - by Bioz Stars, 2026-09
93/100 stars
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Effect of TAMs phenotype on immunologic efficacy in EC patients and proliferation and migration for Ishikawa cells. A Immunohistochemistry (IHC) staining of M1-like TAMs (CD86 + ) and M2-like TAMs (CD206 + ) in tumor tissues from EC patients. The bar graphs on the right show the IHC staining scores (*** P ≤ 0.001). B Flow cytometry analysis of M1-like TAMs (CD86 + ) and M2-like TAMs (CD206 + ) in PBMCs. The left panel shows representative flow plots, and the bar graphs on the right quantify the percentages (*** P ≤ 0.001). C Experimental design for TAMs polarization and co-culture system. D CCK-8 assay measuring cell viability of Ishikawa cells co-cultured with M1 and M2-like TAMs for 24, 48 and 72 h. The bar graphs show the percentage of viable cells (* P < 0.05, ** P ≤ 0.01). E Transwell migration assay of Ishikawa cells co-cultured with M1 and M2-like TAMs. The bar graphs on the right quantify the number of migrated cells (* P < 0.05, ** P ≤ 0.01)

Journal: Discover Oncology

Article Title: Integrating multi-omics data to optimize immunotherapy in endometrial cancer: a comprehensive study

doi: 10.1007/s12672-025-02978-2

Figure Lengend Snippet: Effect of TAMs phenotype on immunologic efficacy in EC patients and proliferation and migration for Ishikawa cells. A Immunohistochemistry (IHC) staining of M1-like TAMs (CD86 + ) and M2-like TAMs (CD206 + ) in tumor tissues from EC patients. The bar graphs on the right show the IHC staining scores (*** P ≤ 0.001). B Flow cytometry analysis of M1-like TAMs (CD86 + ) and M2-like TAMs (CD206 + ) in PBMCs. The left panel shows representative flow plots, and the bar graphs on the right quantify the percentages (*** P ≤ 0.001). C Experimental design for TAMs polarization and co-culture system. D CCK-8 assay measuring cell viability of Ishikawa cells co-cultured with M1 and M2-like TAMs for 24, 48 and 72 h. The bar graphs show the percentage of viable cells (* P < 0.05, ** P ≤ 0.01). E Transwell migration assay of Ishikawa cells co-cultured with M1 and M2-like TAMs. The bar graphs on the right quantify the number of migrated cells (* P < 0.05, ** P ≤ 0.01)

Article Snippet: The cells were then stained with 4 μL of CD206 antibody (FITC-65155, Proteintech, Hunan, China) and 5 μL of CD86 antibody (PE-65165, Proteintech, Hunan, China), followed by 40 min of incubation in the dark at 4 °C.

Techniques: Migration, Immunohistochemistry, Flow Cytometry, Co-Culture Assay, CCK-8 Assay, Cell Culture, Transwell Migration Assay