Journal: Parasites & Vectors

Article Title: Comparative studies of macrophage-biased responses in mice to infection with Toxoplasma gondii ToxoDB #9 strains of different virulence isolated from China

doi: 10.1186/1756-3305-6-308

Figure Lengend Snippet: Cell surface markers in uninfected macrophages, Wh3-Mφ and Wh6-Mφ. (A) After three days postinfection, mice were euthanized and PECs were harvested. Three hours later adherent peritoneal macrophages were collected and stained for F4/80, CD80, CD86 and MHC class II. (B) As in (A) , Wh3-Mφ and Wh6-Mφ were stained for F4/80, PD-L1, PD-L2 and CD206. Flow cytometry graphs show histograms of F4/80-gated macrophages (dotted lines, the isotype control; solid lines, uninfected macrophages; heavy lines, Wh3-Mφ; tinted and hairline, Wh6-Mφ). ( C , D and E ) MFIs of F4/80 (C) , CD80, CD86 and MHC class II molecule (D) , PD-L1, PD-L2 and CD206 (E) on uninfected macrophages, Wh3-Mφ or Wh6-Mφ are displayed ( # p>0.05, * p<0.05, ** p<0.01, *** p<0.001, Wh3-Mφ or Wh6-Mφ vs uninfected Mφ). Data are representative of five independent experiments.

Article Snippet: The antibodies include FITC-conjugated anti-F4/80, PE-Cy5-conjugated anti-MHCII, PE-conjugated PD-L2 (programmed death ligand, PD-L) (eBioscience, USA), PE-conjugated PD-L1, PE-Cy5-conjugated anti-CD80, APC-conjugated anti-CD86 (BD Pharmingen, USA), Alexa Fluor 647-conjugated anti-CD206 (BioLegend, USA), as well as appropriate isotype control antibodies.

Techniques: Staining, Flow Cytometry