Journal: International Journal of Molecular Sciences

Article Title: Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform, CellSaic, in a Rat Congenital Cleft-Jaw Model

doi: 10.3390/ijms22179478

Figure Lengend Snippet: Surface markers of rBMSCs and rDPSCs. Flow cytometry analyses revealed that both cells were positive for CD90, CD73, and CD44 but negative for CD45.

Article Snippet: Using FACSVerse (BD Biosciences, Franklin Lakes, NJ, USA) to analyze the cell suspension, we evaluated the following surface antigens with antibodies: CD44 (Cat#12–0444–80, Thermo Fisher Scientific Inc., Waltham, MA, USA), CD45 (Cat#554878, BD Pharmingen, San Diego, CA, USA), CD73 (Cat#bs-4834r-pe, Bioss Antibodies Inc., Boston, MA, USA), and CD90 (Cat#202526, BioLegend, San Diego, CA, USA).

Techniques: Flow Cytometry

Journal: Medicine

Article Title: Asperosaponin VI induces osteogenic differentiation of human umbilical cord mesenchymal stem cells via the estrogen signaling pathway

doi: 10.1097/MD.0000000000032344

Figure Lengend Snippet: Flow cytometry detection of UC-MSC surface markers. (A) Anti-CD44 monoclonal antibody. (B) Anti-CD31 monoclonal antibody. (C) Anti-CD45 monoclonal antibody. (D) Anti-CD73 monoclonal antibody. UC-MSC = umbilical cord mesenchymal stem cells.

Article Snippet: Anti-CD44-PE, anti-CD31-PE, anti-CD73-PE, and anti-CD45-PE were purchased from SinoBiological (Beijing, China), and the reverse transcription kit was purchased from Axygen (San Francisco, CA).

Techniques: Flow Cytometry

Journal: Stem Cells and Development

Article Title: The Role of CCL5 in the Ability of Adipose Tissue-Derived Mesenchymal Stem Cells to Support Repair of Ischemic Regions

doi: 10.1089/scd.2013.0307

Figure Lengend Snippet: Analysis of cell surface marker expression. FACS analyses of cell surface markers on these MSC. BM- (A), DT- (B), and AT-MSC (C) were analyzed for the expression of cell surface markers [CD13, CD14, CD31, CD34, CD45, CD73, CD90, CD105, CD166, human leukocyte antigen (HLA)-ABC, HLA-DR, and SSEA4]. These MSC expressed characteristic MSC markers, including CD73, CD90, CD105, CD166, and lacked expression of hematopoietic lineage and endothelial markers.

Article Snippet: MSC were stained with various combinations of monoclonal antibodies conjugated with fluorescein isothiocyanate (FITC), phycoerythrin (PE), and allophycocyanine (APC): FITC-labeled HLA-ABC (W6/32), FITC-labeled CD90 (5E10), PE-labeled anti-CD31 (WM59), and APC-labeled anti-CD45 (HI30) (BioLegend, San Diego, CA); FITC-labeled anti-CD105 (SN6) (AbD Serotec, Oxford, United Kingdom); PE-labeled anti-CD166 (3A6), anti-CD73 (AD2), anti-CD14 (M5E2), and APC-labeled anti-CD34 (581) and anti-mouse IgG (BD Biosciences).

Techniques: Marker, Expressing

Journal: iScience

Article Title: Identification of optimal conditions for human placental explant culture and extracellular vesicle release

doi: 10.1016/j.isci.2023.108046

Figure Lengend Snippet:

Article Snippet: Human anti-CD73 PE , Cellarcus Biosciences , Cat# CBS81-PE-100T.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Proliferation Assay, LDH Cytotoxicity Assay, Software