Journal: International Journal of Oral Science

Article Title: Nociceptive adenosine A 2A receptor on trigeminal nerves orchestrates CGRP release to regulate the progression of oral squamous cell carcinoma

doi: 10.1038/s41368-024-00308-w

Figure Lengend Snippet: Presence of nociceptive nerves in adenosine-concentrated OSCC. a The upper panel presents the broad view of immunostaining of TRPV1 in the patient cohort collected by West China Hospital of Stomatology (WCHS). Scale bar: 2 mm. The lower panel denotes representative samples with and without TRPV1 neuronal infiltration (‘TRPV1 positive’ and ‘TRPV1 negative’). Scale bar: 50 µm. b Kaplan–Meier plot delineating survival probability for WCHS-OSCC patients stratified against TRPV1 neuron infiltration in their tumors, n = 111 patients. c Comparison of adenosine concentration in normal epithelium and HSC3 xenograft of mice, represented as net relative fluorescence unit (RFU). n = 3 mice. d Immunostaining of CD73 in human normal epithelium and human OSCC cells. Scale bars, 50 μm. e Kaplan–Meier plot delineating survival probability for TCGA-HNSC patients stratified against NT5E expression in their tumors. NT5E -high and NT5E -low groups are defined as above or below the median of NT5E expression. The TCGA-HNSC cohort database analyzed was updated to March 29th, 2023, n = 518 patients. Statistical analysis was conducted using unpaired Student’s t -test ( c ) and Log-rank test ( b , e )

Article Snippet: For non-fluorescent staining, antigen was retrieved either through 1X Tris-EDTA (for CD73, p-ERK and YAP) or 10 mM citric acid (for TRPV1and Ki67) before incubated with corresponding primary antibody TRPV1 (Alomone labs ACC-030), CD73(CST 13160 S), p-ERK (CST 4370 S), Ki67 (HUABIO HA721115) and YAP (CST 14074) in blocking solution, followed by reaction enhancer solution and horseradish peroxidase (HRP)-conjugated secondary antibody (ORIGENE PV-9001).

Techniques: Immunostaining, Comparison, Concentration Assay, Fluorescence, Expressing

Journal: iScience

Article Title: GRHL2 suppression of NT5E/CD73 in breast cancer cells modulates CD73-mediated adenosine production and T cell recruitment

doi: 10.1016/j.isci.2024.109738

Figure Lengend Snippet:

Article Snippet: Anti-5′-Nucleotidase Clone 4G6E3 , Novus Bio , Cat# NBP2-37271.

Techniques: Recombinant, Blocking Assay, Western Blot, Cell Isolation, RNA Sequencing Assay, CRISPR, Plasmid Preparation, Software

Journal: Journal of Tissue Engineering

Article Title: Therapeutic potential of epiphyseal growth plate cells for bone regeneration in an osteoporosis model

doi: 10.1177/20417314221116754

Figure Lengend Snippet: Cellular characterization of EGPCs and BM-MSCs: (a) observation of cell morphology and measurement of cell shape (length to width ratio), (b) the proliferation rates of EGPCs and BM-MSCs were measured from day 1 to day 7 by the CCK-8, (c) cell-cycle analysis of BM-MSCs and EGPCs by using propidium-iodide staining. Quantitation of the cells at each-cycle phase, and (d) Flow-cytometric analyses for the surface markers on BM-MSCs and EGPCs. Representative flow-cytometry profiles are depicted for CD73, CD44, CD90, and CD200. Black and red plots represent the negative control and experimental samples, respectively.

Article Snippet: Antibodies against CD73 (NBP2-25235; 5′-Nucleotidase/CD73 Antibody, NOVUS BIOLOGICALS), CD44 (MCA643GA; MOUSE ANTI RAT CD44, Bio-Rad, UK), CD90 (sc-53456; Thy-1/CD90 antibody (aTHy-1A1), Santa Cruz Biotechnology), and CD200 (MA1-70035; CD200 Monoclonal Antibody (OX-2), ThermoFisher Scientific) were used as the primary antibodies (1:100 diluted in the FACS buffer).

Techniques: CCK-8 Assay, Cell Cycle Assay, Staining, Quantitation Assay, Flow Cytometry, Negative Control