Journal: Investigative Ophthalmology & Visual Science

Article Title: Human Lacrimal Gland Derived Mesenchymal Stem Cells – Isolation, Propagation, and Characterization

doi: 10.1167/iovs.64.10.12

Figure Lengend Snippet: List of Primers Used For Gene Expression Analysis

Article Snippet: The expression of designated markers, CD73 (1:200, 13160; Cell Signalling Technology, Danvers, MA, USA), CD90 (1:200, ab181469; Abcam, UK), CD105 (1:200, sc-376381; Santa Cruz Biotechnology, USA), CD45 (1:200, 13197; Cell Signalling Technology, USA), HLA-DR (1:200, ab55152; Abcam, UK) and Vimentin (1:200, sc-6260; Santa Cruz Biotechnology) was additionally confirmed by immunofluorescence.

Techniques: Gene Expression, Sequencing

Journal: Investigative Ophthalmology & Visual Science

Article Title: Human Lacrimal Gland Derived Mesenchymal Stem Cells – Isolation, Propagation, and Characterization

doi: 10.1167/iovs.64.10.12

Figure Lengend Snippet: Flow cytometric analysis of cell surface antigens was performed using BM-MSCs and LG-stromal cells in the P3 and the positive expression of CD73 ( P = 0.25), CD90 ( P = 0.42), and CD105 ( P = 0.003) was detected, whereas lacking expression of negative markers CD34 ( P = 0.95), CD45 ( P = 0.09), and HLA-DR ( P = 0.02).

Article Snippet: The expression of designated markers, CD73 (1:200, 13160; Cell Signalling Technology, Danvers, MA, USA), CD90 (1:200, ab181469; Abcam, UK), CD105 (1:200, sc-376381; Santa Cruz Biotechnology, USA), CD45 (1:200, 13197; Cell Signalling Technology, USA), HLA-DR (1:200, ab55152; Abcam, UK) and Vimentin (1:200, sc-6260; Santa Cruz Biotechnology) was additionally confirmed by immunofluorescence.

Techniques: Expressing

Journal: Investigative Ophthalmology & Visual Science

Article Title: Human Lacrimal Gland Derived Mesenchymal Stem Cells – Isolation, Propagation, and Characterization

doi: 10.1167/iovs.64.10.12

Figure Lengend Snippet: Immunophenotypic analysis of BM-MSCs and LG-stromal cells. ( A ) MSCs showed positive expression for CD73, CD90, CD105, and Vimentin, and negative for CD45 and HLA-DR with Alexa fluor 594 conjugated secondary antibody. The nucleus was counterstained with DAPI. Images were captured at 40X magnification. ( B ) Quantitative analysis of the expression of various markers of BM-MSCs and LG- stromal cells in terms of corrected total cell fluorescence intensity. Data are shown as absorbance mean ± standard deviation from three separate experiments. ( C ) The quantitative analysis of the percentage of cells expressing various markers per area. Representative data of three areas taken for quantification. * P ≤ 0.033, and ** P ≤ 0.002. ( D ) Immunofluorescence staining of LG epithelium specific markers (C-Kit, Lysozyme, Pan CK ) in human LG tissue and LG-Stromal cells. * In images indicates blood vessels in the LG tissue sections.

Article Snippet: The expression of designated markers, CD73 (1:200, 13160; Cell Signalling Technology, Danvers, MA, USA), CD90 (1:200, ab181469; Abcam, UK), CD105 (1:200, sc-376381; Santa Cruz Biotechnology, USA), CD45 (1:200, 13197; Cell Signalling Technology, USA), HLA-DR (1:200, ab55152; Abcam, UK) and Vimentin (1:200, sc-6260; Santa Cruz Biotechnology) was additionally confirmed by immunofluorescence.

Techniques: Expressing, Fluorescence, Standard Deviation, Immunofluorescence, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Human placenta-derived mesenchymal stem cells stimulate neuronal regeneration by promoting axon growth and restoring neuronal activity

doi: 10.3389/fcell.2023.1328261

Figure Lengend Snippet: Characterization of hPMSCs. (A) Morphology at passage 3. Both normoxic and hypoxic cultures show highly homogeneous spindle-like shape. Scale bar: 500 µm (B) Proliferation capacity. Cumulative population doublings of hPMSCs grown under hypoxia (square) are higher than cultures grown in normoxia (dot). (C) Flow cytometry shows specific hMSCs marker expression pattern: positive for CD105, CD44, CD90, CD73 and negative for CD14, CD19, CD34, CD45 and HLA-DR. (D) Osteogenic differentiation was confirmed by Alizarin red staining of calcium deposits. (E) Adipogenesis differentiation was followed by Oil Red O staining of lipids vacuoles (black arrows indicate lipid droplets). (F) Alcian Blue staining of proteoglycans demonstrated chondrogenesis differentiation. Scale bar: 100 μm. Two-way ANOVA and post hoc Sidak test for multiple comparisons between means (** p ≤ 0.005; * p ≤ 0.05).

Article Snippet: Antibodies against the following human antigens were used: CD105-FITC (Miltenyi Biotect, Bergisch Gladbach, Germany, cat# 130-112-327, 1:50), CD90-FITC (Miltenyi Biotec, cat# 130-114-901, 1:50), CD44-VioBlue (Miltenyi Biotec, cat# 130-113-906, 1:50), CD73-APC (Miltenyi Biotec, cat# 130-111-909, 1:50), MSC Phenotyping Cocktail-PE (CD34, CD14, CD19, CD45, Miltenyi Biotec cat# 130-125-285, dilution according to the manufacturer’s instructions).

Techniques: Flow Cytometry, Marker, Expressing, Staining