Journal: Physiological Reports

Article Title: Autologous minced muscle grafts improve endogenous fracture healing and muscle strength after musculoskeletal trauma

doi: 10.14814/phy2.13362

Figure Lengend Snippet: Flow cyotometry antibodies

Article Snippet: CD68 , ED1 , Novus Biologicals , NB600‐985V , 0.001 mg/mL.

Techniques: Concentration Assay

Journal: Cell reports

Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice

doi: 10.1016/j.celrep.2025.116841

Figure Lengend Snippet: Representative images and percent area densities of (A) CD68, (B) Iba1, (C) Gfap, (D) Clec7a, and (E) Tmem119 in the hippocampus and entorhinal/piriform cortical area (EC/PC area) of 9.5-month-old male PCfD mice treated with OIL or TAM at 3 months of age ( n = 16–20 per group). Scale bar: 500 μm. Mann-Whitney tests were used to determine statistical significance (CD68 hippocampus, p = 0.0222). Data are represented as the mean ± SEM. * p < 0.05.

Article Snippet: The primary antibodies were diluted as follows: rabbit- anti -Iba1 (FUJIFILM Wako, 019–19741, 1:500), rat- anti -CD68 (BioRad, MCA1957, 1:500), and guinea pig anti-Plin2 (Progen, GP40, 1:200).

Techniques: MANN-WHITNEY

Journal: Cell reports

Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice

doi: 10.1016/j.celrep.2025.116841

Figure Lengend Snippet: (A) Left: illustration showing approximate locations of the z stacks acquired for the neutral lipid imaging experiments. This image was generated using BioRender. Right: representative 3D full-thickness Imaris renderings of Iba1, CD68, and BODIPY co-stains of PCfD TAM and OIL hippocampi. All channels were masked outside of Iba1 surfaces to promote visualization of cells throughout the entire stack. Scale bar: 25 μm. Inset scale bar: 10 μm. (B) Quantification of Iba1-CD68-BODIPY colocalized volumes per total Iba1 volume. Significance was determined with a Mann-Whitney test ( n = 17–20 per group, p = 0.015). Data are represented as the mean ± SEM. (C) Volcano plot of lipid species altered between PCfD TAM and OIL groups. Statistically significantly altered species are colored ( n = 10 per group). (D) Heatmap of lipid species extracted from bulk posterior cortices of 9.5-month-old male mice via liquid chromatography-mass spectrometry (LC-MS). * p < 0.05.

Article Snippet: The primary antibodies were diluted as follows: rabbit- anti -Iba1 (FUJIFILM Wako, 019–19741, 1:500), rat- anti -CD68 (BioRad, MCA1957, 1:500), and guinea pig anti-Plin2 (Progen, GP40, 1:200).

Techniques: Imaging, Generated, MANN-WHITNEY, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

Journal: Cell reports

Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice

doi: 10.1016/j.celrep.2025.116841

Figure Lengend Snippet: (A) Volcano plot of lipid species altered between CD11b high /CD45 int brain myeloid cells from male and female 9.5-month-old WT and PS19 mice (without floxed DGAT alleles) previously gavaged with tamoxifen at 3 months of age. Statistically significantly altered species are colored ( n = 13–14 per group, sexes combined). (B) Representative high-resolution (100×) image of Iba1, BODIPY, CD68, and Plin2 immunofluorescent stains in the hippocampus of a 9-month-old male standard PS19 mouse without floxed DGAT alleles or any treatments. Scale bar: 10 μm. We observed this general staining pattern at 63× in dozens of PS19 and WT mice of multiple strain backgrounds, including PCfD mice treated with TAM and OIL. (C) Representative TEM images of brain myeloid cells from dentate gyrus granule cell layers of 10-month-old male WT and PS19 mice. Myeloid cells were identified by the presence of heterochromatin around the edges of irregularly shaped nuclei. Left: low-magnification images (3,000×, scale bar: 2 μm). Right: high-magnification views (15,000×, scale bar: 400 nm) of lipid-laden lysosomes highlighted in the dashed squares. The red arrows indicate single lysosomal membranes, and the white arrows indicate double lysosomal membranes associated with lipid accumulation. Images are representative of what we have observed from reviewing 6–8 images per sample from 3 PS19 and 2 WT male mice.

Article Snippet: The primary antibodies were diluted as follows: rabbit- anti -Iba1 (FUJIFILM Wako, 019–19741, 1:500), rat- anti -CD68 (BioRad, MCA1957, 1:500), and guinea pig anti-Plin2 (Progen, GP40, 1:200).

Techniques: Staining

Journal: PLOS One

Article Title: Photobiomodulation therapy increases neural stem cell pool in aged 3xTg-AD mice

doi: 10.1371/journal.pone.0321668

Figure Lengend Snippet: A. Representative confocal images of Aβ plaques (6E10), microglia (Iba1) and CD68 showing diffuse microglial phagocytosis away from plaques in sham compared to localized phagocytosis around plaques in PBM-treated animals. B. Quantitative analysis of total and activated microglia show no overall differences in the neuroinflammatory environment surrounding Aβ plaques. (Unpaired t-test). C. Analysis of the distribution of CD68+ microglia shows a trend of higher concentration of phagocytic cells in contact with plaques, and decreasing phagocytosis away from plaques. (Two-way ANOVA).

Article Snippet: Up to six sections representing different regions of the hippocampus were immunostained with the following primary antibodies: Sox2 (Abcam), doublecortin (DCX, Santa Cruz), calretinin (CR, SWANT), β-amyloid (6E10, Biolegend), phospho-tau (pTau, AT8, Invitrogen), βIII-tubulin (Abcam), Iba1 (Synaptic Systems), and CD68 (Rockland).

Techniques: Concentration Assay