Journal: Acta Neuropathologica Communications

Article Title: Humanized tau antibodies promote tau uptake by human microglia without any increase of inflammation

doi: 10.1186/s40478-020-00948-z

Figure Lengend Snippet: Mouse neonatal primary microglia express microglia-specific markers in culture. a Representative fluorescent photomicrographs showing cell morphology of cultured mouse neonatal primary microglia cells. Green, ionizing calcium-binding adaptor molecule 1 (Iba-1), and blue, DAPI nuclear staining. Scale bars, 50 μm. b Flow cytometry analysis confirmed purity of primary microglia culture. Histogram shows the binding for anti-CD11b compared to the binding for nonspecific IgG. Quantification of four independent microglia cultures confirmed that around 95% of cells were positive for microglia marker CD11b (mean +/− SEM). c Analysis of FcγRs confirmed that almost all microglia express FcγI receptor (CD64), FcγII and FcγIII receptor (CD16/32) (mean +/− SEM)

Article Snippet: Anti-human antibodies CD11b-PE-Vio770, CD64-Vio615, CD32-PE, CD16-VioBright and controls: REA control-Vio770, REA control-Vio615, REA control –PE, REA control-VioBright (MACS Miltenyi Biotec), anti-Iba1 (WAKO) were used for human microglia.

Techniques: Cell Culture, Binding Assay, Staining, Flow Cytometry, Marker

Journal: Acta Neuropathologica Communications

Article Title: Humanized tau antibodies promote tau uptake by human microglia without any increase of inflammation

doi: 10.1186/s40478-020-00948-z

Figure Lengend Snippet: Human adult primary microglia express specific microglial markers. a Representative fluorescent photomicrographs of human primary microglia culture demonstrating cell morphology and purity of culture; Iba-1 (green), DAPI nuclear staining (blue). Scale bar represents 50 μm. b Flow cytometry analysis for CD11b, CD16, CD32 and CD64 confirmed purity of human microglia cultures. For each marker representative histograms are shown from one human microglia culture and bar graphs with percentage of positive cells from four independent microglia cultures (mean +/− SEM). Histograms and bar graphs show binding and cell positivity for anti-CD11b, −CD16, −CD32 and -CD64, respectively, compared to binding and cell positivity for nonspecific IgG

Article Snippet: Anti-human antibodies CD11b-PE-Vio770, CD64-Vio615, CD32-PE, CD16-VioBright and controls: REA control-Vio770, REA control-Vio615, REA control –PE, REA control-VioBright (MACS Miltenyi Biotec), anti-Iba1 (WAKO) were used for human microglia.

Techniques: Staining, Flow Cytometry, Marker, Binding Assay

Journal: Frontiers in Nutrition

Article Title: Butyric Acid Ameliorates Myocardial Fibrosis by Regulating M1/M2 Polarization of Macrophages and Promoting Recovery of Mitochondrial Function

doi: 10.3389/fnut.2022.875473

Figure Lengend Snippet: Influences of butyrate on M1/M2 polarization of rat macrophages RMa-bm. (A–D) . Flow cytometry was performed to detect CD86 (+), CD80 (+), CD64 (+), CD16/32 (+), CD163 (+), CD206 (+), TNF-α (+), and IL-10 (+) positive cell rates in different treatment groups, respectively. ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Antibodies CD86 (11-0862-820, ebioscience), CD80 (12-0800-82, ebioscience), CD64 (orb124535, biorbyt), CD16/32 (12-0161-82, ebioscience), CD206 (12-2061-82, ebioscience), and CD163 (11-1631-82, ebioscience) were added to each tube, and stained for 30 min at room temperature without light.

Techniques: Flow Cytometry